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Light microscopy is the most widely used form of microscopy for life science research. The correct handling of the microscope and optimising image quality (e.g. contrast, resolution) as well as experimental design according to different applications are crucial to obtain the best results.
This course will provide the participants with an overview of the basics of microscopy (light and optics, contrast techniques and imaging). The confocal part will include multicolour, 3D, and time-lapse imaging as well as spectral imaging, photobleaching (FRAP, FLIP, FLAP) and photoactivation techniques. Basic image analysis tools including measurement functions, colocalisation, and spectral unmixing as well as 3D/4D image processing will be covered, both in theory and practice. The course will combine lectures with intense daily hands-on practices followed by discussions.
This course is directed towards PhD students and faculty searching for instruction on light microscopy and basic image processing.
Participants will get an overview of the basics of microscopy.
Please see EMBL’s COVID-19 safety recommendations for testing and other requirements to attend this on-site course.
This course is organised in cooperation with Evident-Olympus.
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