Overview
Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.
Registration is not yet open for this event. If you are interested in receiving more information please register your interest.
Course overview
The mammary epithelium is a unique organ in that the majority of its development occurs postnatally. This, combined with ease of experimental access and a stereotypical growth pattern, make it an ideal tissue for studying cellular homeostasis, branching morphogenesis, hormonal effects, stem cell biology and breast cancer. This theoretical and practical course will present techniques for the dissection and dissociation of different subpopulations of both mouse and human mammary, including stem/progenitor, cells. It will also include flow cytometry to separate single cell populations for the consequent in vitro (mammosphere and 3D cultures) and in vivo (mouse mammary fat-pad clearing and cell transplantation) analysis to assess stem cell activity and gene function.
Audience
This course targets PhD students, post-docs and other scientists entering the field of mammary gland biology and breast cancer or those who wish to complement their knowledge using mouse or human methods.
Modules/resources
This theoretical and practical course will present techniques for the dissection and dissociation of different subpopulation of both mouse and human mammary cells, including stem/progenitor cells. It will also include flow cytometry to separate single cell populations for the consequent in vitro (mammosphere and 3D cultures) and in vivo (mouse mammary fat pad clearing and cell transplantation) analysis to assess stem cell activity and gene function. Finally, single cells and cell structures will be analysed by cell imaging, which takes advantage of the extraordinary facilities and expertise at EMBL-HD and constitutes a strong component of the course.
Learning outcomes
The participants will learn about the complete workflow for mammary gland studies and gain an understanding about possibilities and limitations of working with mouse and human systems.n in that course.
Sponsors
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
Warning
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
- EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
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