University of California, San Diego
This conference will take place at EMBL Heidelberg, with the option to attend virtually. Please see EMBL’s COVID-19 safety policy for testing and other requirements if attending the on-site event.
There is an increasing awareness that mechanistic structural information on molecular machines is ideally generated within the context of intact cells in order to fully grasp how they function in a complex native environment. Recent technological advances have nurtured the development of such in situ structural biology approaches in several disciplines, including cryo-EM, mass spectrometry, super-resolution microscopy, NMR, computational modelling and others.
This conference will bring together experts and early stage researchers from the separate fields
“The conference was fantastic, with an amazing list of speakers. As an early career researcher I found these events very inspirational and broadening for research opportunities later.” Kamila Kamuda, University College London, UK
“I immensely benefited from the workshop organized by EMBL through talks of so many brilliant scientists who had explained the fast-evolving technique to capture the whole cell structure at the near-atomic resolution.” – Samar Ballabha Mohapatra, Indian Institute of Technology, India
University of California, San Diego
Max Planck Institute of Biochemistry
Research Institute of Molecular Pathology (IMP)
SLAC National Accelerator Laboratory
University of Basel
University of Cologne
University of California, San Diego
University of Utah
University of Pennsylvania
Leibniz-Forschungsinstitut for Molecular Pharmacology (FMP)
UMass Chan Medical School
University of California, Berkeley
Niels Bohr Institute
Max Delbrück Center for Molecular Medicine
Allen Institute for Cell Science
The Hebrew University of Jerusalem
Weizmann Institute of Science
HHMI Janelia Research
University of Oxford
Max Planck Institute of Biophysics
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|11:30 – 13:00||Registration and light refreshments|
|13:00 – 13:15||Opening remarks by scientific organisers|
|13:15 – 14:00||Keynote|
Rommie Amaro – University of California, USA
Introduced by Jan Kosiski
|14:00 – 17:00||Session 1: in situ cryo-EM|
Session chair: Julia Mahamid
|14:00 – 14:25||In situ multi-scale structural analysis of virus infection by cellular cryo-tomography|
Peijun Zhang – University of Oxford, UK
**Live stream only**
|14:25 – 14:40||Cryo-electron tomography and deep learning denoising reveal native chromatin landscapes of interphase nuclei|
Mikhail Elfsov – IGBMC, France
|14:40 – 14:55||Plate-like guanine biocrystals form via templated nucleation of crystal leaflets on preassembled scaffolds|
Zohar Eyal – Weizmann Institute of Science, Israel
|14:55 – 15:40||Coffee Break and Meet the speakers|
|15:40 – 16:05||Visualizing ribosome biogenesis in cells with 2DTM|
Bronwyn Lucas – University of Massachusetts, USA
|16:05 – 16:20||Enabling discovery with in-cell cryo-ET & alphaFold: identification of a novel translocation complex|
Rasmus Jensen – EMBL Heidelberg, Germany
|16:20 – 16:35||Structural insight into the Ebola virus nucleocapsid assembly cascade by in-cell cryo-correlative light and electron microscopy|
Melina Vallbracht – Heidelberg University Hospital, Germany
|16:35 – 17:00||Exploring photosynthesis and carbon fixation across scales with cryo-electron tomography|
Ben Engel – University of Basel, Switzerland
|17:00 – 17:40||Flash talks|
#46 – Gülberk Bayraktar, #48 – Tiana Behr, #52 – Daksh Daksh, # 60 – Chinenye Ekemezie, #74 – Andreja Kezar, # 77 – Matthew Lycas, #82 – Evangelia Nathanail, #84 – Alexander Rau, #85 – Karolina Roganowicz, #89 – Simon Schaefer, #94 – Eva Maria Soriano Jerez, #95 – Marie-Christin Spindler, #97 – Reiya Taniguchi, #102 – Renjing Wang, #103 – Helena Watson, #106 – Davide Zabeo, #110 – Michael Zimmermann,
|17:40 – 19:00||Dinner in Canteen|
|19:00 – 20:30||Welcome reception and tour of Imaging Centre|
|08:15 – 09:15||Arrival|
|09:15 – 12:00||Session 2: Advances in super-resolution light microscopy|
Session chair: Suliana Manley
|09:15 – 09:40||Visualizing the inner life of cells with super-resolution microscopy|
Melike Lakadamyali – University of Pennsylvania, USA
|09:40 – 09:55||4Pi-STORM visualization of nuclear pore complex architecture|
Mark Bates – Institute for Nanophotonics, Germany
**Live stream only**
|09:55 – 10:10||Visualization of loop extrusion by nanoscale 3D DNA tracing in single human cells|
Kai Beckwith – EMBL Heidelberg, Germany
|10:10 – 10:40||Coffee Break and Meet the speakers|
|10:40 – 11:05||Accessing nanoscale structure and dynamics with light|
Francisco Balzarotti – Research Institute of Molecular Pathology, Austria
**Live stream only**
|11:05 – 11:20||Toward correlative cryogenic super-resolution light and electron microscopy|
Hisham Mazal – Max Planck Institute, Germany
|11:20 – 11:35||Superresolution microscopy reveals partial preassembly and subsequent bending of the clathrin coat during endocytosis|
Aline Tschanz – EMBL Heidelberg, Germany
|11:35 – 12:00||Bringing light to mitochondrial apoptosis|
Ana Garcia-Saez – University of Cologne, Germany
**Live stream only**
|12:00 – 13:30||Lunch, including job fair.|
|13:30-16:30||Session 3: Modeling across scales|
Session chair: Rommie Amaro
|13:30 – 13:55||Integrative structure modeling of protein complexes in the age of deep learning |
Dina Schneidman-Duhovny – The Hebrew University of Jerusalem, Israel
|13:55 – 14:10||Towards visual proteomics: In-situ detection of macromolecular complexes by high-resolution template matching|
Sergio Cuz-Leon – Max Planck Institute of Biophysics, Germany
|14:10 – 14:25||Combining computation and microscopy to reveal mechanisms in bacterial transport machines|
Angela Kirykowicz – University of Cambridge, UK
|14:25 – 15:10||Coffee break and meet the speakers|
|15:10 – 15:35||Computational microscopy of biomembrane shapes|
Weria Pezeshkian – Niels Bohr Institute, Denmark
|15:35 – 15:50||Predicting lipid-protein interactions in the age of integrative structural biology|
Stefano Vanni – University of Fribourg, Switzerland
|15:50 – 16:05||Validation of integrative structures in PDB-Dev based on SAXS, CX-MS, and 3DEM data|
Arthur Zalevsky – University of California, USA
|16:05 – 16:30||Animating Data-Rich Molecular Mechanisms |
Janet Iwasa – University of Utah, USA
|16:30 – 17:05||Flash talks|
#54 – Sofia De Felice, #55 – Takahiro Deguchi, #67 – Jan Philipp Kreysing, #69 – Nazia Hussain, #79 – Johannes Rudolph, #87 – Jenny Sachweh, #101 – Christian Tueting, #109 – Liv Zimmermann, #47 – Paul Becker, #49 – Harald Bernhard, #51 – Cristina Capitanio, #93 – Deniz Daviran, #57 – Joe Dobbs, #61 – Bronwen Foster, #73 – Mukthi Iyer
|17:00 – 18:30||Poster Session 1 (odd) with beer and snacks|
The poster listing can be found here
|18:30 – 20:00||Dinner in Canteen|
|20:00 – 22:00||Pub Quiz and social drinks|
|08:15 – 09:15||Arrival|
|09:15 – 12:00||Session 4: Emerging technologies|
Session chair: Martin Beck
|09:15 – 09:40||What in-cell NMR can teach us about intracellular protein structures and dynamics.|
Phil Selenko – Weizmann Institute of Science, Israel
|09:40 – 09:55||Live-cell imaging and integrative modeling to explore the functionality of protein assemblies|
Altair Chinchilla Hernandez – Pompeu Fabra University, Spain
|09:55 – 10:10||Cryo-EM snapshots of eukaryotic membrane proteins in native lipid-bilayer nanodiscs|
Kevin Janson – Martin Luther University Halle-Wittenberg, Germany
|10:10 – 10:40||Coffee Break and meet the speakers|
|10:40 – 11:05||Integrated intracellular organization and its variations in human iPS cells |
Susanne Rafelski – Allen Institute for Cell Science
|11:05 – 11:20||Linking cellular ultrastructure to gene expression with correlative cryo-ET/scRNA-seq|
Andre Schwarz – Max Planck Institute for Brain Research, Germany
|11:20 – 11:35||Exploiting OMVs for the in-situ characterization of bacterial envelope proteins|
Johannes Thoma – University of Gothenburg, Sweden
**live stream only**
|11:35 – 12:00||Protein structures in context with proteome-wide biophysics|
Ilaria Piazza – Max Delbrück Center for Molecular Medicine, Germany
**Live stream only**
|12:00 – 13:30||Lunch|
|13:30-16:45||Session 5: Instrumentation|
Session chair: Jurgen Plitzko
|13:30 – 14:15||Keynote |
The long and winding road to in situ structural biology with cryo-ET
Juergen Plitzko – Max Planck Institute of Biochemistry, Germany
Introduced by: Julia Mahamid
|14:15 – 14:30||Plasma FIB milling for the determination of structures in situ|
Casper Berger – Rosalind Franklin Institute, UK
|14:30 – 14:45||Cryo-FIB milling and cryo-ET of vertically trapped, dividing E. coli bacteria|
Victoria Hale – MRC Laboratory of Molecular Biology, UK
|14:45 – 15:30||Coffee Break and Meet the speakers|
|15:30 – 15:55||Dynamics of molecules at the nanoscale with photo-switching |
Ilaria Testa – SciLifeLab, Sweden
|15:55 – 16:20||Super-resolved cryogenic correlative light and electron microscopy: Specific labelling with cellular context|
Peter Dahlberg – Stanford, USA
|16:20 – 16:45||Laser phase plate: Advancing beyond proof-of-concept|
Holger Müller – University of California, Berkeley, USA
|16:45 – 17:20||Flash talks|
#44 – Milan Aleksic #56 – Natalie Diepenhorst, #58 – Wioleta Dudka, #62 – Herman Fung, #66 – Miguel Hernandez Gonzalez, #76 – Sven Klumpe, #68 – Neil Mcdonald, #83 – Alessandro Passera, #86 – Max Ruwolt, #88 – Veijo Salo, #92 – Ioannis Skalidis, #96 – Frosina Stojanovska, #100 – Zuzana Trebichalská, #104 – Yu-Le Wu, #108 – Christian Zimmerli
|17:20 – 18:45||Poster Session 2 (even) with beer and snacks|
The poster listing can be found here
|18:45 – 20:15||CONFERENCE DINNER in the canteen|
|20:15 – 22:45||Live music|
|08:15 – 09:15||Arrival|
|09:15 – 12:30||Session 6: Across scales|
Session chair: Philipp Selenko
|09:15 – 09:40||Developing structural interactomics and its application in cell biology|
Fan Liu – Leibniz-Forschungsinstitut for Molecular Pharmacology, Germany
|09:40 – 09:55||Integrative structural studies of thyroid homeostasis: a first look into cellular and organoids organisation|
Francesca Coscia – Human Technopole, Italy
|09:55 – 10:20||Towards automated segmentation in volume electron microscopy|
Aubrey Weigel – Institute of Genetics and Molecular and Cellular Biology, USA
|10:20 – 10:50||Coffee Break and Meet the speakers|
|10:50 – 11:05||Bacterial nano-compartments, their function in metal homeostasis and role in the cellular response mechanisms against stress|
Célia Maria Romão – Universidade Nova de Lisboa, Portugal
|11:05 – 11:20||How influenza A virus solves the nuclear escape room|
Daniel Ziemianowicz – EMBL Hamburg, Germany
|11:20 – 11:45||Building a mind for cancer|
Trey Ideker – University of California, USA
|11:45 – 12:30||Round table discussion|
|12:30 – 12:45||Closing remarks and poster prize|
On-site registration fees include admission, conference materials, COVID-19 safety measures, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.
Virtual registration fees include access to all of the talks (livestreamed and on demand) and facility to submit questions.
|On-site PhD Student||€350|
|Virtual PhD Student||€125|
NO visa support letters will be issued until payment of the registration fee is confirmed.
Accredited journalists may be eligible to register for a reduced press rate or in some cases for complimentary registration. Registrants may be required to provide accreditation or equivalent proof of press membership after registration. Please contact Christopher Stocks for more information. Please note that we do not offer complimentary registrations for editors of scientific journals.
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As further changes in our events are possible due to COVID-19, you should book flights, trains and hotels with flexible options and favourable cancellation conditions.
|Hotel Anlage||Hotel ibis Heidelberg||Premier Inn Heidelberg City|
|Hotel Bayrischer Hof||ISG Hotel||Staycity Aparthotel Heidelberg|
|B&B Hotel||Leonardo Hotel Heidelberg City Centre||Steffi’s Hostel Heidelberg|
|Exzellenz Hotel||Meininger Heidelberg||Hotel Vier Jahreszeiten Heidelberg|
|Hotel Holländer Hof||Hotel Monpti|
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Download the conference bus schedule here. If you miss the bus you can call a taxi at +49 6221 302030
Address: EMBL, Meyerhofstraße 1, 69117 Heidelberg, Germany. For further information on getting to EMBL Heidelberg visit Public Transportation to the Venue. For information about accommodation and local transportation please refer to the FAQ page.
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|Good morning||Guten Morgen|
|Good afternoon||Guten Tag|
|Good evening||Guten Abend|
|Good night||Gute Nacht|
|I’m sorry||Tut mir leid|
|Excuse me…||Entschuldigen Sie|
|How are you?||Wie gehts?|
|I’m fine thanks. And you?||Mir geht es gut , danke, und dir/Ihnen?|
|What is your name||Wie heisst du? Wie heissen Sie?|
|My name is||Ich heisse|
|Do you speak English||Sprechen Sie Englisch?|
|I don’t understand||Ich verstehe nicht|
|Please speak more slowly||Können Sie bitte langsamer sprechen|
|Where is the toilet?||Wo ist die Toilette?|
|Please call me a taxi||Bitte rufen Sie mir ein Taxi|
|How do I get to….?||Wie komme ich zum/zur…..?|
|A beer/two beers please||Ein Bier/zwei Bier bitte|
|A glass of red/white wine please||Ein Glas Rot/Weisswein bitte|
|The menu, please||Die Speisekarte, bitte|
|Is there a local speciality?||Gibt es eine Spezialität aus dieser Gegend?|
|I’m Vegetarian||Ich bin Vegetarier|
|It was delicious||Es war hervorragend|
|The bill, please||Die Rechnung, bitte|
|I have a headache||Ich habe Kopfschmerzen|
|I have a sore throat||Ich habe Halsschmerzen|
|My stomach hurts||Ich habe Magenschmerzen|
|I’m allergic to||Ich bin allergisch gegen|
|I need a doctor who speaks English||Gibt es einen Arzt, der Englisch spricht?|
Please note that only on-site participants are able to submit abstracts and participate in the poster sessions.
We are using an event platform for this conference. More information about the platform will be shared ahead of the conference.
Additional information can be found in our Code of Conduct.
It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in the conference platform for you to enjoy during the event.
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Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
EMBO reports, an EMBO Press journal
International Union of Biochemistry and Molecular Biology
Open Biology, a Royal Society journal
Journal of Structural Biology, an Elsevier journal
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Date: 8 - 11 Feb 2023
Location: EMBL Heidelberg and Virtual
Venue: EMBL Advanced Training Centre
Abstract submission: Closed
Registration (On-site): Closed
Registration (Virtual): Closed
Contact: Chris Stocks
EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme