Humanized mice: immunotherapy and regenerative medicine

Time (Europe/Berlin)	Session	Location
11:45 – 12:00	Bus from hotel to EMBL ATC Entrance	ISG Hotel
12:00 – 13:00	Arrival & registrations with light lunch	Flex Lab A & B
13:00 – 13:30	Welcome & introductions	Flex Lab A & B
13:30 – 15:00	Flash Talks	Flex Lab A & B
15:00 – 16:00	Lecture: Humanized mice past, present and future and Q&A Leonard Shultz – The Jackson Laboratory, USA	Flex Lab A & B
16:00 – 16:20	Bus to city centre	ATC Entrance
16:20 – 18:00	Free time	Heidelberg City Centre
18:00 – 19:00	Guided tour	Heidelberg City Centre
19:00	Bus to hotel and dinner	ISG Hotel

Time (Europe/Berlin)	Session	Location
08:40 – 08:55	Bus from hotel to EMBL ATC Entrance	ISG Hotel
08:55 – 09:00	Overview of the day	Flex Lab A & B
09:00 – 09:45	Lecture: Development of human T and NK cells in HSC-humanized mice Michael Brehm – UMass Chan Medical School, USA	Flex Lab A & B
09:45 – 10:30	Lecure: HSC-humanized mouse models and reconstitution of human innate cells and lymphatic tissues James DiSanto, Pasteur Institute, France	Flex Lab A & B
10:30 – 11:00	Coffee break	Flex Lab A & B
11:00 – 11:45	Lecture: Human T cell development in immune deficient mice Bart Vandekerckhove, University of Ghent, Belgium	Flex Lab A & B
11:45 – 12:30	Lecture: Human B cell development and tolerance in humanized mice Roberta Pelanda, University of Colorado Anschutz Medical Campus, USA	Flex Lab A & B
12:30 – 13:30	Lunch	EMBL Canteen
13:30 – 13:45	Live Q&A to pre-recorded practical introduction: Refresher course mouse handling and tissue collections Ernesto de la Cueva – EMBL Heidelberg, Germany	Flex Lab A & B
13:45 – 14:00	Live Q&A to pre-recorded practical introduction: Refresher course flow cytometry analyses Daniel Gimenes – EMBL Heidelberg, Germany	Flex Lab A & B
14:00 – 14:15	Live Q&A to pre-recorded practical introduction and protocol: Standardization of multicolor flow cytometry panels, staining techniques and minimal information for standardizations Sebastian Theobald– University Hospital Cologne, Germany	Flex Lab A & B
14:15 – 18:00	Practical session 1: Animal handling, Immune Reconstitution, Flow Cytometry Groups 1 and 2: Blood Group 3 and 4: Spleen Group 5 and 6: Bone Marrow Discussion of results Trainers: Ernesto de la Cueva, Dr. Daniel Gimenes, Dr. Sebastian Theobald, Dr. Li-Chin Yao, Dr. Ali Roghanian Helpers: Dr. Milita Darguzyte, Dr. Sandra Petrus, Dr. Lisa Rieble	Training Lab B
18:00 – 19:30	Meet the speaker – round table discussion (Chairs Michael Brehm and Julie Lang) Vegetarian pizza dinner	ATC Rooftop Lounge
19:30 – 21:30	Poster session 1 (odd numbers)	TBC
21:30	Bus to hotel	ATC Entrance

Time (Europe/Berlin)	Session	Location
08:40 – 08:55	Bus from hotel to EMBL ATC Entrance	ISG Hotel
08:55 – 09:00	Overview of the day	Flex Lab A & B
09:00 – 09:45	Lecture: Acute leukemia models, genomics and tumor microenvironment Jan Jakob Schuringa – University of Groningen, The Netherlands	Flex Lab A & B
09:45 – 10:30	Lecture: PBMC-humanized mouse models assessing safety profiles and cytokine release syndrome James Keck, The Jackson Laboratory, Sacramento, USA	Flex Lab A & B
10:30 – 11:00	Coffee break	Flex Lab A & B
11:00 – 11:45	Lecture: PDX Models of Glioblastoma and oncolytic viral therapies Marta Alonso – University of Navarra, Spain	Flex Lab A & B
11:45 – 12:30	Lecture: Defining tumors for pharmacologic developments Alessandra Piersigilli, Takeda, USA	Flex Lab A & B
12:30 – 13:30	Lunch	EMBL Canteen
13:30 – 13:45	Live Q&A to pre-recorded practical introduction: Implantation, collection and processing of tumors Li-Chin Yao, The Jackson Laboratory, USA	Flex Lab A & B
13:45 – 14:00	Live Q&A to pre-recorded practical introduction: Advanced histopathology: Analytical t-SNE (t-distributed Stochastic Neighbor Embedding), immune cell imaging in the tumor environment, location, organization, interaction, quantitation) Daniel Bachurski, University Hospital Cologne, Germany	Flex Lab A & B
14:00 – 18:00	Practical session 2: Principles and deeper look on xenograft tumor models (tumor Implantation and biopsies) and histopathology Trainers: Dr. Christine Bruss, Dr. Daniel Bachurski, Dr. Li-Chin Yao, Dr. Anja Wege, Dr. Ali Roghanian Helpers: Dr. Milita Darguzyte, Dr. Sandra Petrus, Dr. Lisa Rieble	Training Lab B
18:00 – 19:30	Round table discussion (Chairs J.J. Schuringa and Marta Alonso) Tapas dinner	ATC Rooftop Lounge
19:30 – 21:30	Poster session 2 (even numbers) 5 minutes presentation and 5 minutes questions per person	TBC
21:30	Bus to hotel	ATC Entrance

Time (Europe/Berlin)	Session	Location
08:40 – 08:55	Bus from hotel to EMBL ATC Entrance	ISG Hotel
08:55 – 09:00	Overview of the day	Flex Lab A & B
09:00 – 09:45	Lecture: Applications of Humanized Platforms in Immuno-Oncology Brian Soper – The Jackson Laboratory, USA	Flex Lab A & B
09:45 – 10:15	Lecture: Breast cancer models for immuno-oncology research Anja Wege – University Hospital Regensburg, Germany	Flex Lab A & B
10:15 – 11:00	Coffee break and group photo	TBC
11:00 – 11:45	Lecture: Application of humanized mouse models in drug discovery Johannes Sam – Roche Zürich, Switzerland	Flex Lab A & B
11:45 – 12:30	Lecture: Neonatal tissue-based models for biosimilar research, CRS (Virtual) Kristina Howard – Center for Drug Evaluation and Research, USA	Flex Lab A & B
12:30 – 13:30	Lunch	EMBL Canteen
13:30 – 14:15	Lecture: Development of human B cells and immuno-oncology aspects Julie Lang, University of Colorado, USA	Flex Lab A & B
14:15 – 14:30	Live Q&A to pre-recorded practical introduction: Testing monoclonal antibodies: schedules and dose finding Ali Roghanian, Univ. Southampton, UK	Flex Lab A & B
14:30 – 18:30	Practical session 3: Combined humanization (including neonate models) and xenograft tumor models – Immuno-Oncology studies by flow cytometry and histopathology Analyses Trainers: Dr. Christine Bruss, Dr. Daniel Bachurski, Dr. Li-Chin Yao, Dr. Anja Wege, Dr. Sebastian Theobald Helpers: Dr. Milita Darguzyte, Dr. Sandra Petrus, Dr. Lisa Rieble	Training Lab B
18:30 – 19:30	Bus to hotel and dinner	ISG Hotel
19:30 – 21:30	Meet the speaker – round table	ISG Hotel

Time (Europe/Berlin)	Session	Location
08:40 – 08:55	Bus from hotel to EMBL ATC Entrance	ISG Hotel
08:55 – 09:00	Overview of the day	Flex Lab A & B
09:00 – 09:45	Lecture: Regenerative Medicine: Gene-modification of hematopoietic cells with lentiviral vectors and gene editing against cancer and pathologies Renata Stripecke, University Hospital of Cologne, Germany	Flex Lab A & B
09:45 – 10:30	Lecture: Gene Editing of hematopoietic stem cells and T cells for clinical uses Toni Cathomen, University of Freiburg, Germany	Flex Lab A & B
10:30 – 11:00	Coffee break	Flex Lab A & B
11:00 – 11:15	Live Discussion and Q&A to pre-recorded lecture: Development of human organoids and use in humanized mouse models Sandra Petrus, University of Cambridge, UK	Flex Lab A & B
11:15 – 12:00	Lecture: Human liver chimeric mouse models – the chances & challenges Karl-Dimiter Bissig, Duke University Medical Center, USA	Flex Lab A & B
12:00 – 12:15	Live Q&A to pre-recorded practical introduction: Gene editing molecular tools and methods Jamal Alzubi, University of Freiburg, Germany	Flex Lab A & B
12:15 – 12:30	Live Q&A to pre-recorded practical introduction: Gene editing standard operating procedures for practical class Renata Stripecke, University Hospital of Cologne, Germany	Flex Lab A & B
12:00 – 13:00	Lunch	EMBL Canteen
13:00 – 17:00	Practical 4: Engineering the grafts via non-viral CRISPR/Cas gene editing knock-outs and knock-ins Groups 1 and 2: Gene editing hematopoietic stem cells Groups 3 and 4: Gene editing T cells Groups 5 and 6: Gene editing tumor cells Flow cytometry analyses of pre-prepared samples Trainers: Dr. Jamal Azubi, Dr. Renata Stripecke, Dr. Ali Roghanian Helpers: Dr. Milita Darguzyte, Dr. Sandra Petrus, Dr. Lisa Rieble	Flex Lab A & B
17:00 – 18:00	Bus to Downtown (stop at ISG Hotel)	ATC Entrance
18:00 – 22:00	Downtown dinner	TBC

Time (Europe/Berlin)	Session	Location
08:40 – 08:55	Bus from hotel to EMBL ATC Entrance	ISG Hotel
08:55 – 09:00	Overview of the day	Flex Lab A & B
09:00 – 09:45	Lecture: Infections of immunocompromised hosts, EBV and KSV Christian Münz, University of Zürich, Switzerland	Flex Lab A & B
09:45 – 10:30	Lecture: Humanized mice to study emerging viral infections Estefania Rodriguez – Bernhard Nocht Institute for Tropical Medicine, Germany	Flex Lab A & B
10:30 – 11:00	Coffee break	Flex Lab A & B
11:00 – 11:45	Lecture: Infection of humanized mice with HIV and development of new therapies Bruce Torbett, University of Washington, USA	Flex Lab A & B
11:45 – 12:30	Lecture to be confirmed	Flex Lab A & B
12:30 – 14:30	Packed Lunch Final discussions, Feedback & Closing remarks	Flex Lab A & B
14:30	Bust to Hotel and Heidelberg train station	ATC Entrance

The course is limited to 24 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, meals and coffee breaks. This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue.

Academia	€500
PhD Student	€500
Industry	€1000

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

• The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
• If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
• If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants and childcare grants. 

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €500 for any participant travelling to an EMBO Practical Course.

– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.

– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.

*Registration fees are only covered for EMBO Practical Courses

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Application

You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of registration fee waivers and travel grants during the motivation letter or abstract selection process. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.

Hotel

Accommodation in twin rooms, including breakfast, is included in the registration fee. Accommodation has been booked for you at the ISG Hotel for the default dates of 16 to 21 November (6 nights). In case you would like to have a single room, please let the hotel know and note that you will be required to pay directly an additional cost of €24 per night (availability of single rooms may be limited). Any additional nights need to be booked and paid for by you. We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL Merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire …

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

• Map to walk from ISG Hotel to EMBL

Additional information

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

• ATC orientation map

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

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Technical requirements

Below you will find the software and technical requirements for this course.

• Participants need to bring their own laptops, which will be used to remotely access EMBL virtual machines with pre-installed software.
• You will need admin rights to install the software required to access the virtual machines. If you do not have admin rights, please contact your technical support team in advance to make sure the software can be installed.
• If you have a non-standard HDMI port (other than type A), you need to bring an adaptor.
• Keyboard and PC mouse are available for use with a standard USB port. If you have a different port type, such as USB-C, you will need to bring your own.

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.