FISHing for RNAs: classical to single molecule approaches

Time (Europe/Berlin)	Speaker	Location
13:30 – 15:00	Familiarise with EMBL eCampus platform, and prime on pre-course work, exercises and learning materials Get access to pre-course material Pre-course material: – Pre-recorded lecture 1 by Emerald Perlas on Histology overview and applications – Pre-recorded lecture 2 by Florence Besse on Subcellular localization of mRNAs in neuronal memory circuits – Pre-recorded lecture 3 by Imre Gaspar on RNA detection methods Video and study material related to practical sessions: – Practical 1: Conventional ISH: 2,5 hours – Practical 2: Probe design: 3 hours – Practical 3: smFISH and HCR-ISH: 2,5 hours – Practical 5: Image analysis: 3 hours	Online on EMBL eCampus

Time (Europe/Berlin)	Session	Location
13:00 – 13:30	Welcome coffee	ISG Hotel
13:30 – 14:00	Welcome, house notes and course overview	ISG Hotel
14:00 – 15:30	Ice breaker: Flash talks and activity in groups	ISG Hotel
15:30 – 16:15	Keynote lecture: SmFISH in low and high throughput unravels gene expression mechanisms Edouard Bertrand – Institute of Human Genetics, France	ISG Hotel
16:15 – 17:00	Discussion Session: Pre-recorded talk on Histology overview and applications Emerald Perlas – EMBL Rome, Italy	ISG Hotel
17:00 – 17:30	Coffee break	ISG Hotel
17:30 – 18:15	Discussion session: Pre-recorded talk on Subcellular localization of mRNAs in neuronal memory circuits  Florence Besse – Institut de Biologie Valrose, France	ISG Hotel
18:15 – 19:00	Discussion session: Pre-recorded talk on RNA detection methods Imre Gaspar – Turbine.ai, Hungary	ISG Hotel
19:00 – 20:30	Dinner	ISG Hotel

Time (Europe/Berlin)	Session	Location
08:40	Bus from hotel to EMBL ATC Entrance	ISG Hotel bus stop
09:00 – 09:10	Overview of the day Imre Gaspar – Turbine.ai, Hungary	Courtyard Room A & B
09:10 – 09:30	Lab safety instructions	Courtyard Room A & B
09:30 – 10:00	Practical: Probe design and production Theory and demo of probe design Imre Gaspar – Turbine.ai, Hungary	Training Lab A & B
10:00 – 10:45	Practical: Theory and demo of smFISH and HCR-ISH Imre Gaspar – Turbine.ai, Hungary Nadia Formicola – CBI Toulouse, France	Training Lab A & B
10:45 – 11:00	Coffee break	Training Lab A & B
11:00 – 11:45	Practical: Probe design and production (continued) Probe preparation (ddUTP + dye conjugation) Imre Gaspar – Turbine.ai, Hungary	Training Lab A & B
11:45 – 12:30	Keynote lecture: RNA localization, spatial transcriptomics Andreas Moor – ETH Zürich, Switzerland	Courtyard Room A & B
12:30 – 13:30	Lunch break	EMBL canteen
13:30 – 14:15	Practical: Conventional ISH Introduction and theory of conventional ISH Emerald Perlas – EMBL Rome, Italy Chiara Capponi – University of Rome, La Sapienza, Italy Sara Di Persio – Münster University Hospital, Germany	Training Lab A & B
14:15 – 16:15	Practical: Conventional ISH (continued) (section pre-treatment, Proteinase K digest, acetylation, hybridization set-up) Emerald Perlas – EMBL Rome, Italy Chiara Capponi – University of Rome, La Sapienza, Italy Sara Di Persio – Münster University Hospital, Germany with flexible coffee break	Training Lab A & B
16:15 – 16:45	Practical: smFISH + HCR-ISH 3a: sm FISH: Probe labeling set-up Imre Gaspar – Turbine.ai, Hungary Florence Besse – Institut de Biologie Valrose, France Nadia Formicola – CBI Toulouse, France	Training Lab A & B
16:45 – 17:00	Coffee break	ATC Rooftop lounge
17:00 – 18:30	Student presentations	ATC Rooftop lounge
18:30 – 20:00	Dinner with discussion on career topics	ATC Rooftop lounge
20:00	Bus to Hotel	EMBL bus stop

Time (Europe/Berlin)	Session	Location
08:40	Bus from hotel to EMBL ATC Entrance	ISG Hotel bus stop
08:50 – 09:00	Overview of the day Emerald Perlas – EMBL Rome, Italy	Courtyard Room A & B
09:00 – 10:00	Practical: Conventional ISH (continued) Post-hybridisation washes Emerald Perlas – EMBL Rome, Italy Chiara Capponi – University of Rome, La Sapienza, Italy Sara Di Persio – Münster University Hospital, Germany	Training Lab A & B
10:00 – 10:45	Keynote lecture: in vivo imaging of RNA biogenesis Franka Voigt – Universität Zürich, Switzerland	Courtyard Room A & B
10:45 – 11:00	Coffee break	Courtyard Room A & B
11:00 – 12:00	Practical: Conventional ISH (continued) Staining set-up Emerald Perlas – EMBL Rome, Italy Chiara Capponi – University of Rome, La Sapienza, Italy Sara Di Persio – Münster University Hospital, Germany	Training Lab A & B
12:00 – 13:00	Lunch with discussion on next career steps	EMBL canteen
13:00 – 15:15	Practical: smFISH + HCR-ISH (continued) 3a and b: sm FISH: probe purification and QC and HCR_ISH specimen pre-work and hybridization setup Imre Gaspar – Turbine.ai, Hungary Florence Besse – Institut de Biologie Valrose, France Nadia Formicola – CBI Toulouse, France	Training Lab A & B
15:15 – 16:15	Practical: Conventional ISH (continued) Washing and mounting of specimen Emerald Perlas – EMBL Rome, Italy Chiara Capponi – University of Rome, La Sapienza, Italy Sara Di Persio – Münster University Hospital, Germany	Training Lab A & B
16:15 – 17:00	Keynote lecture: GE regulation in microbes Evelina Tutucci – Vrije Universiteit Amsterdam, The Netherlands	Courtyard Room A & B
17:00 – 17:30	Coffee break	ATC Rooftop lounge
17:30 – 19:00	Student presentations	ATC Rooftop lounge
19:00 – 20:00	Dinner	ATC Rooftop lounge
20:00	Bus to Hotel	EMBL bus stop

Time (Europe/Berlin)	Session	Location
08:40	Bus from hotel to EMBL ATC Entrance	ISG Hotel bus stop
08:50 – 09:00	Overview of the day Florence Besse – Institut de Biologie Valrose, France	Courtyard Room A & B
09:00 – 11:15	Practical: smFISH + HCR-ISH (continued) 3a and 3b: HCR-ISH: washes smFISH pre-hybridisation set-up for incubation HCR-ISH: Amplification set-up for reaction smFISH hybridisation Imre Gaspar – Turbine.ai, Hungary Florence Besse – Institut de Biologie Valrose, France Nadia Formicolar – CBI Toulouse, France with concurrent coffee break	Training Lab A & B
11:15 – 12:00	Keynote lecture: Data presentation, data integrity Helena Jambor – University of Applied Sciences of the Grisons, Switzerland	Courtyard Room A & B
12:00 – 12:10	Group photo	Courtyard Room A & B
12:10 – 13:00	Lunch break	EMBL canteen
13:00 – 15:30	Practical: Conventional ISH (continued) Conventional ISH imaging Emerald Perlas – EMBL Rome, Italy Chiara Capponi – University of Rome, La Sapienza, Italy Sara Di Persio – Münster University Hospital, Germany With concurrent coffee break	Training Lab A & B
15:30 – 15:45	Coffee break	Courtyard Room A & B
15:45 – 16:30	Keynote lecture: 3D Insights into Octopus Arm Development via Hybridization Chain Reaction Eve Seuntjens – KU Leuven, Belgium	Courtyard Room A & B
16:30 – 18:00	Practical: smFISH + HCR-ISH (continued) 3a and 3b: smFISH & HCR-ISH: washes and specimen mounting Imre Gaspar – Turbine.ai, Hungary Florence Besse – Institut de Biologie Valrose, France Nadia Formicolar – CBI Toulouse, France With concurrent coffee break	Training Lab A & B
18:00 – 19:00	Dinner	EMBL canteen
19:00 – 20:00	Discussion on responsible conduct of research: Experimental design and Standards and controls in the ISH field Moderator: Helena Jambor – University of Applied Sciences of the Grisons, Switzerland	Courtyard Room A & B
20:00	Bus to Hotel	EMBL bus stop

Time (Europe/Berlin)	Session	Location
08:40	Bus from hotel to EMBL ATC Entrance	ISG Hotel bus stop
08:50 – 09:00	Overview of the day	Courtyard Room A & B
09:00 – 10:30	Practical: Imaging in groups (smFISH and HCR-ISH) Imre Gaspar – Turbine.ai, Hungary Florence Besse – Institut de Biologie Valrose, France Nadia Formicolar – CBI Toulouse, France Discussion: Meet the speaker in groups Speakers onsite	ALMF and Courtyard Room A & B
10:30 – 10:45	Coffee break	Courtyard Room A & B
10:45 – 12:15	Practical: Imaging in groups (smFISH and HCR-ISH) continued Imre Gaspar – Turbine.ai, Hungary Florence Besse – Institut de Biologie Valrose, France Nadia Formicolar – CBI Toulouse, France Discussion: Meet the speaker in groups Speakers onsite	ALMF and Courtyard Room A & B
12:15 – 13:15	Lunch	EMBL canteen
13:15 – 14:00	Keynote lecture: RNA/protein phase condensates Mainak Bose – Indian Institute of Technology Kharagpur, India	Courtyard Room A & B
14:00 – 16:00	Practical: ISH image analysis Basics ISH image processing and analysis, part 1 Imre Gaspar – Turbine.ai, Hungary	Computer Training Lab
16:00 – 17:30	Discussion session: Trouble shooting	Courtyard Room A & B
17:30 – 18:00	Bus to downtown Heidelberg	EMBL bus stop
18:00 – 19:30	Heidelberg Guided City Tour	Heidelberg old town
19:30 – 22:00	Downtown Dinner	Oskar Restaurant and Vinotheque

Time (Europe/Berlin)	Session	Location
08:40 – 08:50	Bus from hotel to EMBL ATC Entrance	ISG Hotel
08:50 – 09:00	Overview of the day Elisabeth Zielonka – EMBL Heidelberg, Germany	Computer Training Lab
09:00 – 10:15	Practical: ISH image analysis Basics ISH image processing and analysis, part 2 Imre Gaspar – Turbine.ai, Hungary	Computer Training Lab
10:15 – 10:30	Coffee break	Computer Training Lab
10:30 – 12:00	Practical: ISH image analysis Basics ISH image processing and analysis, part 3 Imre Gaspar – Turbine.ai, Hungary	Computer Training Lab
12:00 – 13:00	Lunch	EMBL canteen
13:00 – 15:30	Group work: Participants preparing group presentations supported by trainers with concurrent coffee break	Courtyard Room A & B
15:30 – 16:30	Discussion: Group Data Presentations incl. Discussions with concurrent coffee break	Courtyard Room A & B
16:30 – 17:00	Wrap up Florence Besse – Institut de Biologie Valrose, France	Courtyard Room A & B
17:00 – 18:00	Bus to ISG hotel and Heidelberg train station	EMBL bus stop
19:00 – 20:00	Dinner	ISG Hotel

The course is limited to 16 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, meals and coffee breaks. This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue

Academia	€500
PhD Student	€500
Industry	€1100

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

• The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
• If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
• If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants and childcare grants. 

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

– up to €500 for any participant travelling to an EMBO Practical Course.

– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.

*Registration fees are only covered for EMBO Practical Courses

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grants

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.

This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.

Selection

The scientific organisers will select the recipients of registration fee waivers during the participant selection process for courses. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Further details

Check out this list of external funding opportunities.

For further information about financial assistance please refer to the FAQ page.

Hotel

Accommodation in twin rooms, including breakfast is included in the registration fee. Accommodation will be booked for the selected participants at the ISG Hotel for the default dates of Sunday 31 August until Saturday 6 September (6 nights).

In case you would like to have a single room, please let the hotel know. You will be required to pay an additional cost of 22.50€ per night (availability of single rooms may be limited). This should be paid by you directly to the ISG Hotel during your stay.

We recommend that all course participants stay at the ISG Hotel for the course.

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk..

EMBL Merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire …

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

• Map to walk from ISG Hotel to EMBL

Additional software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Zoom will be used for the live talks and Q&A sessions. More information will be provided closer to the start of the course.

Other information

While using the software during the course, please make sure you have nothing else using your bandwidth. We recommend using a wired connection.

Please find additional information including FAQs and terms and conditions on our Information for Participants page.