Edit
Seeing is Believing: Imaging the Molecular Processes of Life – Course and Conference Office

EMBO | EMBL Symposium

Seeing is Believing: Imaging the Molecular Processes of Life

Overview

EMBO and EMBL are committed to sharing research advances and sustaining scientific interaction throughout the coronavirus pandemic. We are delighted to announce that this conference is going virtual and invite you to join us online. The virtual conference includes talks from invited speakers, short talk presenters, digital poster sessions, online group discussions and networking opportunities.

For late registrations please contact Iva Gavran (iva.gavran@embl.de).

Symposium Overview

Got something to say? Tweet it! #EESImaging

The molecular processes of life are naturally dynamic in space and time from the atomic to the organismal level. The rapid development of imaging methods across the full scale of biological organisation is revolutionising our ability to visualise the inner workings of macromolecular complexes, organelles, cells, tissues, organs and whole organisms. Being able to see biological processes unfold in real time allows us to understand the mechanisms of life as well as disease.

The symposium will bring together the leading developers of imaging methods with cutting-edge applications that illustrate how imaging can answer biological questions. We will emphasize methods that can capture the dynamics of life, spanning the whole range from molecular resolution to imaging of whole organisms.

From its beginning in 2011, Seeing is Believing has embraced novel imaging technologies that open new windows for biological discovery, including single-molecule and super-resolution, light sheet and correlative light electron microscopy.

In this spirit, in 2021, we will include a session on in situ structural biology, prominently featuring 3D cryo-electron microscopy. Recent exciting examples show that these technologies have the potential to resolve the structures of molecular machines in action in their natural cellular and even organismal context, seamlessly linking it to other scales of imaging in the symposium. Everybody interested in the latest imaging technologies and their applications in the life sciences should attend.

The symposium provides many opportunities for presentations, discussions, and interactions between students, postdocs, junior, and senior investigators.

Session Topics

  • In situ structural biology
  • Super-resolved imaging
  • Probes & biosensors
  • Organismal imaging
  • Correlative imaging
  • Image analysis
  • Discoveries by bioimaging

What Past Participants Say about the Symposium

A great event to immerse yourself in the newest ideas and approaches in bio-imaging.” Andreas Gahlmann, University of Virginia, USA

It’s a fantastic opportunity to find great scientists sharing new insights on technological and biological advancement in a vibrant environment.” Simone Bonaccorsi, UNSW Sydney, Australia

This remains my favourite conference, with the blend of technology and applied sciences, I challenge any scientist not to come out of this meeting feeling exhilarated and full of ideas.” Dr. Ola Sabet, University of Zürich, Switzerland

I learned an exceptional amount about the state of the art in super resolution microscopy and its applications by attending this conference, which would have otherwise taken months to sift through the literature to fine the relevant papers. I’m definitely planning on coming back for the next edition.” Greg McMahon, Principal Research Scientist, National Physical Laboratory Teddington, UK

Seeing is Believing is a fantastic meeting that brings together the exciting, rapidly developing fields of cutting-edge microscopy development and cell biology. Please please please keep organising these meetings, they are very inspirational for young scientists and exceptionally well-received.” Kobus van Unen, Cell Biology Institute, University of Bern, Switzerland

Speakers

Martin Beck

Max Planck Institute of Biophysics

Germany

Florian Jug

Max Planck Institute of Molecular Cell Biology and Genetics

Germany

Dong Li

Chinese Academy of Sciences

China

Prisca Liberali

Friedrich Miescher Institute for Biomedical Research

Switzerland

Suliana Manley

École Polytechnique Fédérale de Lausanne

Switzerland

Mackenzie Mathis

École Polytechnique Fédérale de Lausanne

Switzerland

Gaia Pigino

Max Planck Institute of Molecular Cell Biology and Genetics

Germany

Hari Shroff

National Institute of Biomedical Imaging and Bioengineering

USA

Scientific Organisers

Conference Organisers

Iva Gavran

EMBL Heidelberg

Germany

Elisabeth Wintersteller

EMBL Heidelberg

Germany

Programme

  • The virtual conference includes live-streamed invited speakers and short talk speakers with Q&A sessions after each talk.
  • Information on the live stream and access to the discussion platform and digital posters will be provided shortly before the start of the event.
  • Access to the recorded talks will be available for 2 weeks after the start of the event.

All times in the programme below are shown as the time in Europe/Berlin.

To find out the equivalent time zone in your location, enter Berlin, the programme time and your city into the Time Zone Converter.

Day 0 – Monday, 04 October 2021
Time (Europe/Berlin)Speaker
14:00 – 14:50Pre-symposium webinars:

  • Delmic: Powerful workflows by Delmic
  • CrestOptics: DeepSIM: seamless evolution from Confocal to Super-Resolution
  • PCO: Do pixel size and MTF influence your microscopy, and if so, how?
  • Zeiss: Lattice Lightsheet 7: the new standard for exploring subcellular dynamics
You can read abstracts in Pre-symposium webinars tab
15:00 – 15:50Pre-symposium webinars:

  • Olympus: The Power to See More: VS200 Research Slide Scanner with X Line Objectives
  • Leica: Capture Life as it Happens – Live imaging across scales in space and time
You can read abstracts in Pre-symposium webinars tab
16:00 – 16:50Pre-symposium webinars:

  • Nikon: The axonal cytoskeleton at the nanoscale
  • Omicron
  • Thermo Fisher Scientific: Explore large multi-channel and time-series data with Amira Software and the new Xplore5D extension
You can read abstracts in Pre-symposium webinars tab
17:00 – 17:50Pre-symposium webinars:

  • Bruker: Multiparametric AFM, SMLM and SPIM highspeed Correlative Microscopy Solutions from Bruker Nano Surfaces
  • Dotphoton: Jetraw image compression – Taming the “data beast”
You can read abstracts in Pre-symposium webinars tab
Day 1 – Tuesday, 05 October 2021
Time (Europe/Berlin)Speaker
14:00 – 14:10Opening remarks
14:10 – 16:20Virtual session 1: Super-resolved imaging
14:10 – 14:15Session intro
Chair: Suliana Manley
14:15 – 14:30Invited lecture: Adaptive temporal sampling with an intelligent iSIM
Suliana Manley – École Polytechnique Fédérale de Lausanne, Switzerland
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:30 – 14:45Invited lecture: Live cell compatible super resolution fluorescence microscopy for 3D imaging
Ilaria Testa – SciLifeLab, Sweden
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:45 – 15:00Invited lecture: Accessing the nano-scale with MINFLUX
Francisco Balzarotti – Research Institute of Molecular Pathology, Austria
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:00 – 15:15Invited lecture: All-optical super-resolution imaging of molecules in their nanoscale cellular context
Joerg Bewersdorf – Yale University, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:20 – 15:30Short break
15:30 – 15:45Molecular organization and mechanics of vimentin single filaments revealed by SMLM
Cecile Leduc – Institute Jacques Monod, France
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:45 – 16:00Podosome cluster dynamics unveiled by radial spatiotemporal image correlation spectroscopy and super-resolution imaging
Paul Wiseman – McGill University, Canada
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
16:00 – 16:15Visualizing the native cellular organization by coupling cryo-fixation with expansion microscopy (Cryo-ExM)
Marine Laporte – University of Geneva, Switzerland
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
16:20 – 16:50Meet the speakers of Session 1
Suliana Manley, Ilaria Testa, Francisco Balzarotti, Joerg Bewersdorf, Cecile Leduc, Paul Wiseman, Marine Laporte
16:50 – 18:05Virtual poster session 1
Topic 1: Super-resolved imaging
Topic 2: Correlative imaging
Topic 7: Discoveries by bioimaging (presenters with last name A-F)
18:05 – 20:15Virtual session 2: Correlative imaging
18:05 – 18:10Session intro
Chair: Harald Hess
18:10 – 18:25Invited lecture: 3D Electron Microscopy and Cryo-CLEM
Harald Hess – HHMI-Janelia Research Campus, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
18:25 – 18:40Invited lecture: Multimodality Structured illumination microscopy for super-resolution live-cell imaging
Dong Li – Chinese Academy of Sciences, China
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
18:40 – 18:55Invited lecture: Visualising the architecture of organelle contact sites
Wanda Kukulski – University of Bern, Switzerland
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
18:55 – 19:10Invited lecture: Revealing mitotic chromosome assembly by light- and electron microscopy
Daniel Gerlich – Institute of Molecular Biotechnology, Austria
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:15 – 19:25Short break
19:25 – 19:40Super-resolution and correlative microscopy reveal atypical centrosome organization of the malaria-causing parasite Plasmodium falciparum
Caroline Simon – Heidelberg University Hospital, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:40 – 19:55Measuring conformational changes in clathrin light chain at single sites of endocytosis with FLIM-FRET-CLEM
Kazuki Obashi – NHLBI, NIH, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:55 – 20:10Correlative light and electron microscopy suggests that mutant huntingtin dysregulates the endolysosomal pathway in presymptomatic Huntington’s disease
Ya Zhou – University College London, UK
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
20:15 – 20:45Meet the speakers of Session 2
Harald Hess, Dong Li, Wanda Kukulski, Daniel Gerlich, Caroline Simon, Kazuki Obashi, Ya Zhou
20:45 – 20:50Transition to next session / Break
20:50 – 21:35Virtual speed networking

Day 2 – Wednesday, 06 October 2021
Time (Europe/Berlin)Speaker
13:15 – 13:45Industry session
14:00 – 16:10Virtual session 3: In situ structural biology
14:00 – 14:05Session intro
Chair: Julia Mahamid
14:05 – 14:20Invited lecture: Molecular views into cellular functions by in-cell cryo-electron tomography
Julia Mahamid – EMBL Heidelberg, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:20 – 14:35Invited lecture: Towards a mechanistic understanding of motile and primary cilia with CLEM and cryo-electron tomography
Gaia Pigino – Max Planck Institute of Molecular Cell Biology and Genetics, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:35 – 14:50Invited lecture: Opening Windows into the Cell: Bringing structure to cell biology using Cryo-electron tomography
Elizabeth Villa – University of California, San Diego, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:50 – 15:05Invited lecture: Conformational dynamics of nuclear pore complexes
Martin Beck – Max Planck Institute of Biophysics, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:10 – 15:20Short break
15:20 – 15:35Enterovirus cell entry visualized in situ by cryo-electron microscopy
Pavel Plevka – CEITEC, Masaryk University, Czech Republic
15:35 – 15:50In-cell structural biology of actin filament nucleation by the Arp2/3 complex studied by cryo-electron tomography and subtomogram averaging
Florian Schur – Institute of Science and Technology (IST) Austria, Austria
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:50 – 16:05Motion of single molecular tethers reveals dynamic subdomains at organelle contact sites
Christopher Obara – HHMI – Janelia Research Campus, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
16:10 – 16:40Meet the speakers of Session 3
Julia Mahamid, Gaia Pigino, Elizabeth Villa , Martin Beck , Pavel Plevka, Florian Schur, Christopher Obara
16:40 – 17:55Virtual poster session 2
Topic 3: In situ structural biology
Topic 4: Image analysis
Topic 7: Discoveries by bioimaging (presenters with the last name G-S)
17:55 – 20:05Virtual session 4: Image analysis
17:55 – 18:00Session intro
Chair: Florian Jug
18:00 – 18:15Invited lecture: Microscopy Image Restoration and Downstream Analysis – recent improvements and hopes for the future
Florian Jug – Max Planck Institute of Molecular Cell Biology and Genetics, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
18:15 – 18:30Invited lecture: Using machine learning for measuring movement
Mackenzie Mathis – École Polytechnique Fédérale de Lausanne, Switzerland
(Lecture will be livestreamed only)
18:30- 18:45Invited lecture: Image-based spatiotemporal dissection of the human proteome
Emma Lundberg – KTH Royal Institute of Technology, Sweden
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
18:45 – 19:00Invited lecture: Multiscale intravital fluorescence microscopy
Qionghai Dai – Tsinghua University, China
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:05 -19:15Short break
19:15 – 19:30Invited lecture: The BioImage Archive: Enabling image data publication, sharing and reuse
Matthew Hartley – EMBL-EBI Hinxton, UK
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:30 – 19:45Learning a representation of cellular morphology: Unsupervised deep learning for shape and texture characterization of a cell
Valentyna Zinchenko – EMBL Heidelberg, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:45 – 20:00An interactive deep learning-based approach reveals the organelle ultrastructure
Yusuke Hirabayashi – The University of Tokyo, Japan
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
20:05 – 20:35Meet the speakers of Session 4
Florian Jug, Mackenzie Mathis, Emma Lundberg, Qionghai Dai, Matthew Hartley, Valentyna Zinchenko, Yusuke Hirabayashi
20:35 – 20:40Transition to next session / Break
20:40 – 21:40Quiz icebreaker and Bar mixer
Day 3 – Thursday, 07 October 2021
Time (Europe/Berlin)Speaker
13:15 – 13:45Industry session
14:00 – 16:10Virtual session 5: Organismal imaging
14:00 – 14:05Session intro
Chair: Kate McDole
14:05 – 14:20Invited lecture: Imaging mammalian development with light-sheet microscopy
Kate McDole – MRC Laboratory of Molecular Biology, UK
(Lecture will be livestreamed only)
14:20 – 14:35Invited lecture: New optical tools to interrogate biological function in-vivo
Robert Prevedel – EMBL Heidelberg, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:35 – 14:50Invited lecture: Multiscale organoid imaging
Prisca Liberali – Friedrich Miescher Institute for Biomedical Research, Switzerland
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:50 – 15:05Invited lecture: Enhancing fluorescence microscopy with computation
Hari Shroff – National Institute of Biomedical Imaging and Bioengineering, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:10 – 15:20Short break
15:20 – 15:35Light-sheet microscopy with doubled resolution via multi-directional structured illumination
Reto Fiolka – University of Texas Southwestern Medical Center, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:35 – 15:50All-optical photoacoustic tomography for deep tissue imaging
Jakub Czuchnowski – EMBL Heidelberg, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:50 – 16:05Single Cell Tracking of Human Organoid development Links the Metabolic state to Stemness and Proliferation
Maria Rodriguez Colman – UMC Utrecht, The Netherlands
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
16:10 – 16:40Meet the speakers of Session 5
Kate McDole, Robert Prevedel, Prisca Liberali, Hari Shroff, Reto Fiolka, Jakub Czuchnowski, Maria Rodriguez Colman
16:40 – 17:55Virtual poster session 3
Topic 5: Organismal imaging
Topic 6: Probes & biosensors
Topic 7: Discoveries by bioimaging (presenters with the last name V-Z)
17:55 – 20:05Virtual session 6: Probes & biosensors
17:55 – 18:00Session intro
Chair: Ralf Jungmann
18:00 – 18:15Invited lecture: Hybrid small-molecule:protein based indicators based on far-red fluorophores
Luke Lavis – HHMI-Janelia Research Campus, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
18:15 – 18:30Invited lecture: New fluorescent and bioluminescent probes for live-cell imaging
Kai Johnsson – Max Planck Institute for Medical Research, Germany
(Lecture will be livestreamed only)
18:30 – 18:45Invited lecture: Live imaging in plants using autonomous genetically encoded bioluminescence
Ilia Yampolski – Institute of Bioorganic Chemistry, Russia
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
18:45 – 19:00Invited lecture:
Ralf Jungmann – Max Planck Institute of Biochemistry, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:05 – 19:15Short break
19:15 – 19:30Near infrared imaging of cellular signaling
Sebastian Kruss – Bochum University, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:30 – 19:45Seeing in vivo forces– development of light-producing intracellular nanosensors to quantify mechanical signaling in living cell chromosomes
Maria Mukhina – Harvard University, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
19:45 – 20:00More Than a Label: Fluorescent Nanodiamonds for All-optical Quantum Sensing of Free Radicals in Live Cells
Alina Sigaeva – University Medical Center Groningen, The Netherlands
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
20:05 – 20:35Meet the speakers of Session 6
Luke Lavis, Kai Johnsson, Ilia Yampolski, Ralf Jungmann, Sebastian Kruss, Maria Mukhina, Alina Sigaeva
20:35 – 20:40Transition to next session / Break
20:40 – 21:40Virtual Heidelberg tour
Day 4 – Friday, 08 October 2021
Time (Europe/Berlin)Speaker
13:15 – 13:45Imaging service session:
Euro BioImaging: Revolutionize your research with the best microscopy tools
Johanna Bischof
Introducing the EMBL Imaging Centre
Timo Zimmermann
14:00 – 16:05Virtual session 7: Discoveries by bioimaging
14:00 – 14:05Session intro
Chair: Monica Bettencourt Dias
14:05 – 14:20Invited lecture: Imaging the living cell surface reveals an active actin membrane composite
Satyajit Mayor – National Centre for Biological Sciences, India
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:20 – 14:35Invited lecture: Control of microtubule dynamics: Seeing proteins and drugs in action
Anna Akhmanova – University of Utrecht, The Netherlands
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:35 – 14:50Invited lecture: Centrosomes and Cilia in Development and Disease
Monica Bettencourt Dias – Instituto Gulbenkian de Ciência, Portugal
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
14:50 – 15:05Invited lecture: Visualizing Nuclear Pore Dynamics during Cell Division
Srigokul Upadhyayula – University of California, Berkeley, USA
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:10 – 15:20Short break
15:20 – 15:35Endosomal Escape of delivered mRNA from endosomal recycling Tubules visualized at the Nanoscale by multi-colour SMLM
Christian Franke – Friedrich Schiller University Jena, Germany
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:35 – 15:50Whole-brain activation mapping and connectivity analysis using activity-dependent genetic labeling, that shed light on a new node in stress circuitry
Atsushi Kasai – Osaka University, Japan
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
15:50 – 16:05Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization
Dana Lorber – Weizmann Institute of Science, Israel
(Lecture will be livestreamed and available on demand in Video Library until 22 Oct 2021)
16:05 – 16:10Transition to next session / Break
16:10 – 17:00Closing session
16:10 – 16:15Poster prize award
16:15 – 16:45Poster prize talks
16:45 – 17:00Closing remarks
17:00 – 17:30Meet the speakers of Session 7
Satyajit Mayor, Anna Akhmanova, Monica Bettencourt Dias, Srigokul Upadhyayula, Christian Franke, Atsushi Kasai, Dana Lorber

Sponsors

Gold Sponsor

 

Silver Sponsors

 

Bronze Sponsor

 

Event Sponsor

Intelligent Imaging Innovations

 

Media Partners

EMBO reports, an EMBO Press journal

Imaging & Microscopy, a Wiley journal

International Union of Biochemistry and Molecular Biology

Journal of Cell Science, a The Company of Biologists journal

Microscopy and Analysis, a Wiley journal

Open Biology, a Royal Society Publishing journal

RSC Chemical Biology, Royal Society of Chemistry

Sensors & Diagnostics, Royal Society of Chemistry

 

Sponsorship Opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Warning

EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

  • EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
  • EMBL will never call or email you to ask for your credit card details or to request a payment.
  • All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsoring@embl.de.

Practical Information

Registration Fees and Abstract Submission

Registration Fees (include access to all of the talks, digital poster sessions and online group discussions, and help us cover our costs to run the event. For further information please refer to the FAQ page):

Academia190 Euro
PhD Student140 Euro
Industry240 Euro
EMBL StaffIntranet access

Accredited journalists may be eligible to register for a complimentary registration. Registrants may be required to provide accreditation or equivalent proof of press membership after registration. Please contact the EMBL Course and Conference Officfor more information.

Confirmation and Payment

Registration will be on a first-come first-served basis. Your place can only be confirmed after payment of the registration fee.
Types of payments accepted are international bank transfers and credit card payments.

Abstract submission

Abstract submission for this symposium is closed. For enquiries about submitting a late abstract for a poster presentation, please contact the EMBL Course and Conference Office.

Financial Assistance

Financial Assistance

Registration Fee Waivers

All academic and student registrants are invited to apply for a registration fee waiver, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO. The registration fee waiver covers the registration sum that you have paid to attend the meeting. Conference participants are not required to pre-pay the registration fee to be selected for a fee waiver for a virtual meeting. If you have already paid the registration fee and are awarded a fee waiver, it will be reimbursed after the meeting. Course participants are required to pay the course fee in advance, which will then be reimbursed after the recipient has attended the course.

Childcare Grants

For participants and speakers with childcare responsibilities there is the possibility to apply for a grant, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO, to offset childcare costs incurred when participating at a virtual event. Eligible costs include fees for a babysitter or childcare facility or travel costs for a care giver. Please note that priority will be given to early stage researchers. Costs will be reimbursed after the meeting only once a reimbursement form and original receipts have been received. Attendance at the event is required in order to be eligible to receive the reimbursement. In order to apply for this grant, you must be registered by the abstract submission deadline.

Application

Applications for financial assistance can be submitted via the submission portal* (for the submission of abstracts for conferences or the submission of motivation letters for courses) by completing the Financial Assistance Application Section (underneath the section for entering abstract/motivation letter information). The link to the portal can be found in the registration confirmation email that you will receive after registering for the conference or course.

For conferences, if you are not submitting an abstract, you can still apply for financial assistance in the submission portal. Read the instructions on how to apply for financial assistance.

Note that priority will be given to those submitting an abstract to present at the conference. In your application you will be asked to answer questions regarding your motivation for applying, and, for registration fee waivers, the reasons why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.

Selection

The scientific organisers will select the recipients of registration fee waivers during the abstract selection process for conferences and the participant selection process for courses. Results will be announced approximately 3 – 4 weeks before the event start date. Selection results do not impact your admission to the meeting. Registration fee waiver selection is based on your current work or study location, your motivation for applying, the reasons for needing financial support and the impact this event will have on your career. Childcare grants are allocated based on career stage, with priority given to early stage researchers.

Further details

Check out this list of external funding opportunities or get more information on attending the conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Virtual Participation Guidelines

Guidelines

Please do:

  • Use the event-specific hashtag as communicated during the event for any related tweets
  • Tweet unless the speaker specifically says otherwise
  • Be mindful of unpublished data
  • Be respectful in tone and content

Please don’t:

  • Share live stream or poster session links with others
  • Broadcast the conference to unregistered participants
  • Capture, transmit or redistribute data presented at the meeting unless presenter gives explicit consent
  • Use offensive language in your posts
  • Engage in rudeness or personal attacks

Additional information can be found in our Code of Conduct.

Health and well-being

It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in the events Slack workspace for you to enjoy during the event.

How to ask questions

Questions during and after the talks can be asked in live streaming platform. The chair moderates the questions and shares them with the speaker. If time runs out or you think of a question later, you can use Slack to ask your questions in the dedicated session channels or via direct message.

Time zone

The programme is planned based on the Europe/Berlin time zone, unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accommodate as many time zones as possible when creating the programme. Please take your time zone into consideration when planning your attendance.

Virtual event platforms

We are using a virtual event platform for this conference. More information about the platform will be shared ahead of the conference.

About

EMBO | EMBL Symposia promote scientific communication and collaboration in the European research area. They provide scientists with a platform to discuss and exchange ideas on forward-looking topics and new developments in the life sciences.

Topics emphasise upcoming developments and the interdisciplinary nature of related fields. Jointly funded and organised by EMBO and EMBL – and complementary to their respective courses, workshops, and conference programmes – the symposia promote scientific communication and collaboration.

All symposia are held in the EMBL Advanced Training Centre (ATC) in Heidelberg, Germany, or virtually.

Pre-Symposium Webinars

Pre-symposium webinars will take place on Monday, 04 October 2021 from 14:00 – 17:50 (Berlin/Europe time zone)

Leica: Capture Life as it Happens – Live imaging across scales in space and time

SPEAKERS:

Dr. Julia Roberti, Product Manager Advanced Confocal Imaging
Dr. Irmtraud Steinmetz, Application Manager Advanced Confocal Imaging
Dr. Julia Koenig, Product Manager EM Sample Preparation for Cryo-workflows

ABSTRACT:

Live imaging is transforming our understanding of biological systems, enabling direct observation and quantitative analysis of structure and processes in cells, tissues, and organisms. The STELLARIS confocal platform provides the tools to achieve excellent image quality, spatiotemporal resolution, and functional information in close-to-physiological conditions. In addition, correlative correlative light and electron microscopy allows to directly identify the right cell at the right time and put dynamic live cell data into the ultrastructural context.

In the first part of the session, we will introduce the Stellaris confocal platform. The combination of the new generation white light lasers, acousto-optical beamsplitter (AOBS), and the Power HyD detector family, enables optimal tuning of both excitation and detection to achieve the most efficient signal acquisition in gentle conditions. Our new, proprietary approach to photon counting, Power Counting, significantly improves image contrast and delivers quantitative results. STELLARIS also includes Dynamic Signal Enhancement (DSE) and the new AI Feature AiviaMotion, a powerful digital tool that can increase the signal-to-noise ratio while maintaining temporal resolution. Combined with LIGHTNING, it adapts to the dynamics of the specimen for the best possible spatial and temporal resolution.

To access functional information, the TauSense technology (1) is a new, straightforward way to access lifetime-based information in confocal imaging. Changes in the fluorescence photon arrival times give an extra layer of information to understand the function of molecules within the cellular environment, to increase image quality, and to expand the number of probes that can be visualized in a specimen. The implementation of different tools (TauConstrast, TauGating, TauScan, and TauSeparation) allows to explore this extra dimension of information at different levels and in a guided way.

In the first part of the webinar we will go through the operating principles behind Photon Counting, LIGHTNING with DSE/AiviaMotion and TauSense using different types of samples. We will show how the different tools are integrated in the STELLARIS confocal platform to get insight into the structure and function from live imaging.

KEYWORDS: Live Imaging, Confocal Imaging, Power Counting, Functional Imaging, CLEM, correlative light and electron microscopy

Nikon: The axonal cytoskeleton at the nanoscale

SPEAKER:

Christophe Leterrier, Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France

ABSTRACT:

The intricate morphology and molecular identity of axons is maintained for decades, but also continuously adapts to changes in the environment and activity of neurons. Axons fulfill these paradoxical demands thanks to a unique cytoskeletal organization that ensures the coordinated transport, anchoring and mobility of axonal components. In our lab, we use super-resolution microscopy to map the nanoscale architecture actin-based structures within the axon. In the axon initial segment, a key compartment for the maintenance of neuronal polarity, we resolved a highly organized assembly encompassing the periodic actin/spectrin scaffold and its partners: ankyrin, myosin. We have also visualized new actin structures along the axon shaft: rings, hotspots and trails, and are now exploring their molecular organization and functions as well as the role of actin at presynapses. For this, we develop a combination of versatile labeling, correlative live-cell/super-resolution/electron microscopy and quantitative, deep-learning based analysis that allow for high-content, nanoscale interrogation of the axonal architecture.

Olympus: The Power to See More: VS200 Research Slide Scanner with X Line Objectives

SPEAKERS:

Flavio Giacobone, Olympus Europa SE & Co. KG
Wei Juan Wong, Olympus Soft Imaging Solutions GmbH

ABSTRACT:

Discover Flexible Slide Scanning

The Olympus Slideview VS200 is a flexible solution for high-throughput and high-quality slide scanning. It has five different observation methods – brightfield, fluorescence, darkfield, phase contrast and polarized light – and can automatically handle a mixture of slide sizes from 26 × 76 mm to 102 × 127 mm. Fluorescence multiplexing is also supported, which makes it possible to create images with up to 32 channels with reliable and precise alignment between channels.

Outstanding Image Quality, Reliable Quantification

For crisp, analysis-ready images, the VS200 captures every detail from slides, across all five observation methods. The optical path has been designed specifically to take advantage of Olympus’ new X Line objectives, which overcome the trade-off between resolution, image flatness and color accuracy – delivering improvements in all three aspects simultaneously. Using true magnifications, from 2X to 100X, it also includes automated oil scanning while the stable and accurate focus of the VS200 gives users confidence when scanning slides for quantification purposes.

A Fluid Workflow for Every User

The VS200 is designed for users of all levels. The basic operation mode enables users to acquire images in as little as three clicks, while the expert mode provides experienced users full control, allowing them to set custom settings to different imaging projects. This is particularly useful if an imaging project requires the acquisitions of different sets of slides over a long timeframe. For ease of use and throughput, the VS200 has a robotic automated slide loader that can handle up to 210 slides, also in mixed sizes. The hot-swap function allows users to change slides during scanning, in case of urgent needs. Efficient working is natural, since the VS200 makes it possible to edit scanned images in parallel with ongoing scans. An automated barcode reader provides reliable capture and recording of information about each slide. The VS200 slide scanner software and hardware can be configured and expanded onsite: going from simple brightfield up to multiplex fluorescence scanning, with deconvolution and AI functions, allows facilities to effortlessly follow the constantly evolving application needs.

KEYWORDS: high-throughput, slide scanning, slide imaging, brain research, drug discovery

Omicron Single- and Multi-Wavelength Plug & Play Laser Light Sources for demanding Microscopy applications

SPEAKER:

Ralf Dietzel, Omicron-Laserage Laserprodukte GmbH

ABSTRACT:

Omicron introduces its latest development LaserNest®, a single-mode desktop laser for microscopy. The presentation also includes information about the multi-wavelength laser light engines LightHUB+ and LightHUB Ultra with up to seven wavelengths.

Thermo Fisher Scientific: Explore large multi-channel and time-series data with Amira Software and the new Xplore5D extension

SPEAKER:

Jan Giesebrecht, Supervisor, Product Applications Specialist, Thermo Fisher Scientific

ABSTRACT:

This live webinar will focus on the visualization and image processing of large 3D image data using the new capabilities of the Amira™ Software solution by Thermo Fisher. The head of the webinar will give you the possibility to learn, through a step-bystep demo, the recently added functions of Amira™ and its new Xplore5D extension. XPlore5D offers the fast and easy exploration of large multi-channel and time-series data. We will also highlight and demonstrate Deep Learning based training and prediction modules.

Bruker: Multiparametric AFM, SMLM and SPIM highspeed Correlative Microscopy Solutions from Bruker Nano Surfaces

High-Speed Atomic Force Microscopy and Super-Resolution Optics – Multiparametric Correlative Microscopy Solutions from Bruker Nano Surfaces

SPEAKERS:

Dr. Tanja Neumann, JPK BioAFM, Bruker Nano GmbH,
Andreas Kraus, JPK BioAFM, Bruker Nano GmbH,

ABSTRACT:

Atomic force microscopy (AFM) currently offers premium spatial resolution of the analysed samples while simultaneously being able to correlate topography and mechanics at near native/physiological imaging conditions. The most recent AFM developments have made it further possible to resolve single molecular dynamic processes on the millisecond scale. In turn, the combination with advanced/customised optics leverages the advantages of immunolabelling techniques for truly correlative microscopy.

During this webinar we will give an overview of correlative microscopy applications featuring fast imaging and advanced super-resolution optical setups. We will also show examples of the accuracy of registering/overlay the AFM and optical images on commercially available DNA origami structures with dimensions below the diffraction limit.

We will further introduce how some of the most recent technological developments have led to unprecedented imaging rates in fluid, setting new milestones in high-speed scanning capabilities. Bruker recently launched the fastest commercially available high-speed AFM (NanoRacer®) enabling real-time visualization of dynamic biological processes taking place on the sub-20-milisecond scale. We will introduce the concept and applications of high-speed imaging for real-time visualization of protein binding kinetics, as well as molecular and cellular dynamics.

 

Applying Single Molecule Localization Microscopy to Structures Deep Inside the Sample

SPEAKER:

Dr. Clemens Schneider, Sales product Specialist FM EEMEA, Bruker

ABSTRACT:

Single Molecule Localization Microscopy (SMLM) is a very widely used approach for superresolution microscopy. Compared to intensity-based superresolution microscopy images (like in STED or SIM), the localization data obtained in SMLM experiments open new ways for data processing and analysis. Having the precise 3D localization information of individual dye molecules in the sample can be used efficiently for analysis of resolution, spatial distribution, clusters, true colocalization (of clusters) and much more.

In most commercially available systems, all of these advantages are only exploited in regions close to the cover slip, since TIRF (or “dirty-TIRF”) illumination is used for reducing the background coming from out-of-focus planes. The Bruker Vutara VXL system is different in that respect. The dedicated widefield illumination in combination with the biplane detection module opens up the possibility of acquiring SMLM data more than 30 μm inside the sample.

This workshop will explain the technical requirements for performing SMLM deep inside the sample and demonstrates post-acquisition steps for SMLM data processing and analysis.

 

The Sample Zoo and adequate Light-Sheet Microscope Solutions from Luxendo, Bruker FM

SPEAKER:

Dr. Malte Wachsmuth, Luxendo GmbH, Bruker FM

ABSTRACT:

Light-sheet microscopy (LSFM) is today a well-known and valuable technology to gain new and deep insights of a wide variety of scientific research areas. Sample imaging across scales – with low photo toxicity and low photo bleaching effects at very high speed.

Which LSFM geometry is the most appropriate for your actual sample size, the structure you want to image, and your desired resolution? What are prerequisites to take into account to acquire the most fascinating, high content images?

Which mounting procedures are the most useful for what type of sample? Live samples, fixed samples or cleared samples, they all have specific needs and treats, and these will be addressed.

In this webinar we present an overview of our product portfolio and the key facts regarding lenses, geometries, sample size, mounting techniques, and resolution that characterizes the different specialisations of our light-sheet microscopes.

CrestOptics: DeepSIM: seamless evolution from Confocal to Super-Resolution

SPEAKERS:

Raino Ceccarelli, PhD., Head Of Product Development, CrestOptics
Luca Clario, Business Developer Specialist, CrestOptics

ABSTRACT:

The number of biology-driven publications that use Super-Resolution Structured Illumination Microscopy (SR-SIM) has increased significantly in recent years, indicating the usefulness of the technique as a tool for discovery. The increased use of SR-SIM also highlights the limitations of the current approaches. Revealing more biological complexity requires fast simultaneous acquisition of as many labels as possible, deep inside complex specimens. A major handicap of all SR methods is their susceptibility to aberrations, especially when imaging deeper than 10μm, which impacts contrast and resolution. To enable all researchers to overcome this multidimensional challenge, CrestOptics has developed DeepSIM, the first SR-SIM module that is compatible with all standard up-right or inverted microscopes. Despite being a high-end super resolution system (100nm in lateral X,Y and 300nm axial Z resolution), DeepSIM is as easy to operate as a confocal system – it doesn’t require any special probes and researchers can maintain their standard sample preparation protocols which guarantees a seamless evolution from Confocal to SR. We have also equipped the system with robust system calibration, pre-optimized acquisition modalities and automated data processing to make the system easier to use. We are aware that establishing SR-SIM as a ubiquitous tool for routine life science research applications requires much more than just ergonomic design and intuitive handling. With this in mind, we have developed the DeepSIM to be a truly enabling technology. Performance is combined with the flexibility of a modular, expandable system available either as standalone equipment or SR add-on module for CrestOptics X-light V3 Spinning Disk system. To further expand SR-SIM application for the study of structural dynamics and molecular interactions, CrestOptics has dedicated itself to improving live-cell imaging capabilities, despite non-trivial challenges like increasing temporal resolution and adapting to 3D imaging deep inside complex specimens. The webinar will showcase the new DeepSIM SR module and demonstrate how it can help researchers to obtain truly meaningful and contextualized deep data, even from live samples over 50μm thick.

Delmic: Powerful workflows by Delmic

SPEAKERS:

Marit Smeets, Delmic B.V.
Katherine Lau, Delmic B.V.
Guido Ridolfi, Delmic B.V.

ABSTRACT:

Unlocking the power of cryo-ET

Cryo-electron tomography (cryo-ET) is an extremely powerful technique that allows studies of the cellular content at the nano-scale in a near-native state. However, the current cryo-ET workflow is error-prone resulting in damaged, contaminated and devitrified samples that do not result in useful tomography data. In the workflow, there are two main challenges: keeping the sample ice contamination-free and capturing the region of interest (ROI) in the final sample.

To overcome these problems, we developed two workflow solutions called METEOR and CERES to streamline the entire cryo-ET preparation workflow. METEOR is an integrated fluorescent light microscope (FLM) that greatly enhances the ROI targeting inside the FIB/SEM while reducing the number of handling steps required. The CERES Ice Defence System consists of multiple innovative tools that are tailored to minimize ice contamination at different steps of the workflow. In this webinar we will show how using both CERES and METEOR will increase the efficiency of the entire workflow and dramatically increase the amount of high-quality cryo-ET data that can be turned into new biological insights.

Bridging the resolution gap with large-scale EM

Correlative Light Electron Microscopy is a symbiosis of techniques: fluorescence microscopy has a broad range of capabilities such as detecting specific proteins or molecules and monitoring dynamic processes in live material. EM provides data on the structural basis of these processes, by showing all membranes and structures in the sample. Combined, these two are an extremely useful research tool.

The EM part of the workflow is a bottleneck that typically forces investigators to only focus on a much smaller region of interest, compared to what can be observed with Fluorescence Microscopes. This means that unless a substantial amount of time is spent on EM, the technique is primarily qualitative.

Here we demonstrate how FAST-EM, a 64-beam electron microscope designed to drastically increase imaging throughput while maintaining high image quality, is used to collect images at nm resolution from large tissue samples. The improved throughput and automation of FAST-EM enables a shift towards using EM as a fast quantitative analysis tool. Higher throughput drastically shortens the time needed to acquire project data, and opens the door for projects of unprecedented scale, so to closing the resolution gap between EM and FM.

With FAST-EM we can easily capture with EM mode a FOV that is as large as the FM FoV (100 times faster) and collect detailed information on every labeled spot.

Dotphoton: Jetraw image compression – Taming the “data beast”

SPEAKER:

Bruno Sanguinetti, CTO at Dotphoton

ABSTRACT:

The combination of fast imaging techniques and machine learning are leading to scientific breakthroughs as well as high-impact applications, such as personalized medicine or autonomous driving. They are also generating vast quantities of image data, which is difficult to move around as well as expensive in time, energy and money. Image compression can help, but the usual techniques are not suitable for quantitative data.

Here we present Jetraw, a raw image compressor that preserves the metrological accuracy of raw data, and achieves compression of 8:1 at the speed of 10Gbps networks. This performance is achived through a metrological traceability to the specific image sensor and settings, which gives Jetraw images additional powers, such as physically-accurate data-augmentation and normalization, features that allow to increase, and to test, the reliability of AI algorithms.

PCO: Do pixel size and MTF influence your microscopy, and if so, how?

SPEAKERS:

Henning Ortkrass*, Marcel Müller*, Anders Kokkvoll Engdahl*, Thomas Huser*,
Gerhard Holst**

*Biomolecular Photonics, Department of Physics – D3, University of Bielefeld, Germany;
**Research Department, PCO AG, Kelheim, Germany

ABSTRACT:

There is a trend in microscopes towards larger field-if-view and larger image circles to be able to receive more information with the recording of one image. For that reason camera manufacturers followed in designing and creating new scientific cameras with larger image sensors and diagonals as well. But, not only the diagonal but even more the pixel size has to be selected properly according to the applied microscope magnification. The relationship will be discussed within this webinar.

Further, it is known that all microscope systems have an optical transfer function or modulation transfer function, and so do cameras. But what is the impact on modern microscope methods like single molecule localization microscopy and structured illumination microscopy? To answer this question, a series of experiments has been done with 4 scientific cameras, which had image sensors with the same pixel size but different MTF. First results are presented within this webinar.

Experimental set-up with mutliple cameras for MTF and SIM measurements
Zeiss: Lattice Lightsheet 7: the new standard for exploring subcellular dynamics

SPEAKER:

Dr. Kirstin Elgass, Business Sector Life Sciences,  ZEISS Research Microscopy Solutions

ABSTRACT:

Since the routine use of fluorescent proteins began in the 1990s, the drive to image live samples has revolutionised our understanding of modern biology. For all live experiments, the most vital consideration is keeping light exposure to an absolute minimum since damage caused by excessive light dose can compromise the integrity of the resulting datasets.

Very often minimising light dosage means reducing the number of acquired images to decrease the length of the timelapse or the frequency of image capture. However, either of these approaches risks missing key events of interest.

Lattice light sheet technology enables 3D acquisition at subcellular resolution and with unprecedented gentleness. First published in 2014, this approach has proven itself to be extremely powerful for long-term studies and homebuilt instruments have generated numerous high impact publications. However, the scope and usability of this approach has been limited since this technology has only been available in an upright configuration and has required significant time and expertise to make the necessary alignments and optimisations for generation of the exciting results.

This has all changed with the release of the ZEISS Lattice Lightsheet 7. This platform provides lattice light sheet imaging but in a convenient, inverted configuration that is compatible with the same samples and sample preparation you would use when imaging with your confocal or spinning disc microscope.

The automatic alignments and easy workflows provided by the inverted Lattice Lightsheet 7 mean that even inexperienced users can now access this cutting-edge approach and capture 3D data of their classically mounted samples over hours and days at a time.

The accessibility of such powerful technology to non-expert users sets this approach to become the new standard in 3D long term timelapse imaging of dish mounted specimens.

Date: 5 - 8 Oct 2021

Location: Virtual


Deadline(s):

Abstract submission: Closed

Registration: Closed


Organisers:

  • Jan Ellenberg
    EMBL Heidelberg, Germany
    • Jennifer Lippincott-Schwartz
      HHMI-Janelia Research Campus, USA
      • Atsushi Miyawaki
        RIKEN Center for Brain Science, Japan

      Contact: Iva Gavran

      Download event poster

      Seeing is Believing Poster

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

Corporate partners

Associate partners

Edit