Hands-on flow cytometry – learning by doing!

Overview

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Course overview

Flow cytometry is a highly effective method for analysing individual cells that has become widely used in the fields of life sciences and medicine. By utilising flow cytometry, researchers can accurately identify and measure specific cell populations within complex mixtures, as well as assess cell cycle progression, proliferation status, and expression of fluorescent markers.

Our course aims to provide a comprehensive understanding of the underlying principles of flow cytometry, while also emphasising practical considerations related to the most frequently used assays. Through a combination of theoretical instruction and hands-on training, students will gain the knowledge and skills necessary to effectively design, execute, and interpret flow cytometry experiments.

Audience

The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of flow cytometry, data acquisition or analysis software (DIVA + FlowJo)  is of advantage, but not strictly a must.

Modules/resources

• In-depth principles of Flow Cytometry
• Quality assessment and instrument validation
• Analysis of cell cycle profiles in fixed cells
• Particles counting
• Apoptosis assays
• Tissue staining of cell surface and intracellular antigens
• Principles and guidelines for experimental design, especially for multi-colour Flow Cytometry
• Essential of gating and gating controls
• Detailed multi-sessions on analysing data from Flow Cytometers in cooperation with FlowJo
• Guidelines to prepare flow cytometry data for publication 

Learning outcomes

Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.

What past participants say about the course

“A fantastic learning experience — perfectly balanced theory and practice, outstanding instructors, and excellent organisation. I highly recommend this course to anyone working with flow cytometry.” – Samuel Bru, Universitat Internacional de Catalunya, Spain

“Coming from Panama, I truly valued the EMBL flow cytometry course. I particularly enjoyed learning from highly experienced experts and having the chance to connect with fellow researchers facing similar challenges.” – Rita Corrales, Gorgas Memorial Institute, Panama

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.