Stability proteomics for assessing the state of the proteome
Our group is participating in the 2022 EIPOD-LinC Fellowship Programme call for applications.
Collaborations that are relevant to the call are listed below. Please contact Mikhail Savitski to discuss your project ideas.
We are looking for a skilled proteomics scientist with crosslinking experience. Currently protein crosslinking is established for highly purified complexes at the EMBL proteomics core facility and is widely used. However, pioneering work has shown that cross linking of proteins in complex mixtures is possible. Here it would be very beneficial to combine sample fractionation approaches that can separate complexes and reduce sample complexity with protein cross linking in order to deduce structural information. Such technology development would enable systematic characterization of protein complexes without the need of in-depth purification. A robust implementation of this workflow would greatly accelerate unbiased protein complex characterization and the resulting service would appeal to a large number of EMBL groups interested in protein complexes and protein interactions as well as the broader scientific community in Europe. In particular, as science moves beyond model organisms such an unbiased technology will accelerate greatly functional characterization of ecologically important, but so far understudied species.
To be able to do this, we will need to establish complementary fractionation techniques for fractionation of whole cell samples of different sources (prokaryotic and eukaryotic cells) and implement crosslinking mass spectrometry in the medium complexity samples collected from the different fractions.
With this line of future research, we aim to strengthen our expertise in development of instrument methods, mass spectrometry and optimization and development of biochemical (fractionation, crosslinking) approaches.