From 3D light to 3D electron microscopy

During this workshop, we will share with participants experiences about sample preparation for the different techniques presented during the conference. We will show tips and tricks for sample preparation and mounting on different supports for 3DEM and CLEM. We will cover the techniques for SBEM sample prep, FIBSEM sample prep (with and without fluorescence preservation) as well as array tomography.​

​Contributing people: ​Christel Genoud, Jean Daraspe, Olivia Muriel Lopez (all University of Lausanne)

See how non-destructive ‘Array Tomography’ (AT) works: This workshop will cover different serial sectioning approaches, strategies for image acquisition, through to aligning the 2D images to be able to generate a 3D model. ​These volume EM datasets can be correlated with 3D light microscopy datasets of the same sample, acquired previously by either confocal imaging of the sample live or fixed, or widefield imaging of the serial sections.​

Contributing people: Jemima Burden (University College London), Ian White (University College London), Raffaela Carzaniga (The Francis Crick Institute), Stephen Furzeland (Carl ZEISS)

We will show you how XRay images can be used to determine the position of pollen tetrads in the arabidopsis anther and how to use this data to trim the sample block to be able to relocate this position in SBF-SEM for imaging of specific pollen tetrads.​

​Contributing people: Anneke Kremer (VIB Ghent), Saskia Lippens (VIB Ghent), Marianne Beckwith (EMBL Heidelberg), Kimberly Meechan (EMBL Heidelberg)

Learn how you can combine cryo-confocal imaging, cryo-lamella preparation and cryo-electron tomography to get high-resolution information of your favorite structure. The correlative workflow connects a confocal fluorescence microscope, with a focused ion beam – scanning electron microscope for targeted lamella preparation and a high-end transmission electron microscope. You will learn about the hardware and software involved to investigate your samples in their native state.​

Contributing people: ​Anna Steyer (EMBL Heidelberg), Simone Mattei (EMBL Heidelberg), Zhengyi Yang (EMBL Heidelberg), Andreas Schertel (Carl Zeiss Microscopy GmbH)

This workshop will present a correlative 3D light and electron microscopy workflow that integrates in-resin confocal fluorescence microscopy, 2-photon branding and focused ion beam scanning electron microscopy (FIB-SEM), as described in Ronchi et al., 2021. We will discuss how to preserve fluorescence during sample preparation for volume EM and show how to target the FIB-SEM acquisition of specific cells of interest based on a fluorescence 3D map acquired by confocal imaging of the block.​

Contributing people: Paolo Ronchi (EMBL Heidelberg), Giulia Mizzon (Heidelberg University), Manuel Gunkel (EMBL Heidelberg), Aliaksandr Halavatyi (EMBL Heidelberg), Karel Mocaer (EMBL Heidelberg)

We will present the typical serial section acquisition workflow with a multibeam scanning electron microscope, where navigation and ROI definition is routinely done on a correlated wide-field light microscope overview image of the sample. A semi-automated Experiment Wizard guides the user step-by-step through the workflow setup, emphasizing the ease-of-use required for a true high-throughput imaging experience. Besides that, operation principle and image data structure of the ZEISS MultiSEM will be introduced.​

​Contributing people: ​Anna Lena Eberle, Tomasz Garbowski, Stefan Nickell (all Carl Zeiss MultiSEM GmbH)

Hosted by Lucy Collinson and Yannick Schwab

The discussion will be moderated by international panellists all involved in community efforts around vCLEM. They will share their views and trigger feedback from the audience around 3 topics: how international communities organise access to infrastructures (open facilities, funding mechanisms); how they develop their training and outreach activities; how they help scientists with dealing with their data (standards, repositories).

Panel members:

Kedar Narayan – NIH National Cancer Institute Center for Cancer Research, USA
Erin Telley (Tranfield) – Instituto Gulbenkian de Ciência, Portugal
Roger Wepf – The University of Queensland, Australia
Johanna Bischof – Euro-BioImaging ERIC, Germany
Paul Verkade – University of Bristol, UK
Jemima Burden – University College London, UK

This conference will be virtual and free to all attendees. For this reason we invite you to register only if you are certain you will attend the event and to use the cancellation option should you register but realise that you cannot attend in the end. 

Accredited journalists may be eligible to register for a complimentary registration. Registrants may be required to provide accreditation or equivalent proof of press membership after registration. Please contact Lea Hohmann for more information.

Confirmation and Payment

Registration will be on a first-come first-served basis. Your place can only be confirmed after payment of the registration fee.
Types of payments accepted are international bank transfers and credit card payments.

Abstract submission

Only registered participants are eligible to submit an abstract. We only accept online abstract submissions.

After you have logged in and successfully registered, you will receive an email asking you to submit your abstract.  Click on the link provided and enter your abstract in the text box provided. Alternatively you can submit your abstract by clicking on the link on the confirmation page directly after registering. The same login credentials are used for both processes.

Please note:

Title: The title should not exceed 20 words. Only the first word of the title should start with a capital letter and the rest of the title should be in lowercase.

Authors and Affiliations: Please fill in the author’s details as requested in the online form. The compulsory details are: First Name, Last Name, Organisation Name (Affiliation or Company), Country and Email. Mark only one author as the role of First author and please don’t forget to indicate who will be presenting. The order of the authors will be listed as follows: First Author, Co-First Author (alphabetically if multiple), co-author(s) (in the order added by the submitter).

Presentation Types: When submitting your abstract, you can apply for an oral or poster presentation. A selection process will take place with the results announced 2-3 weeks after the abstract submission deadline.

Please check our FAQs pages for further information on how to submit an abstract.

Guidelines

• Do not broadcast the conference to unregistered participants.
• You are encouraged to tweet and post about the event. Tweet unless the speaker specifically says otherwise, but be mindful of unpublished data. 
• Please do not capture, transmit or redistribute data presented at the meeting.

Additional information can be found in our Code of Conduct.

Health and well-being

It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in the conference platform for you to enjoy during the event.

How to ask questions

Please use the Q&A function in the event platform.

If you have any other questions, you can go to the Help Desk in the event platform. Click on ‘more’ on the top menu and click Help Desk. 

Time zone

The programme is planned based on the Europe/Berlin time zone, unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accommodate as many time zones as possible when creating the programme. Please take your time zone into consideration when planning your attendance. Remember to set your time zone in your account. 

Virtual event platforms

We are using a virtual event platform for this conference. More information about the platform will be shared ahead of the conference.

Please find additional information including FAQs and terms and conditions on our Information for Participants page.