Single-Cell Omics

Overview

Course Overview

The analysis of single cells advances research in many fields. To properly deal with such minute amounts of sample, each step of the workflow is critical, from sampling to data analysis. Additionally, the integration of data from different approaches is becoming increasingly important to capture the whole picture. This course will cover established techniques from sampling through sequencing, protein analysis and data analysis. Samples will be collected via flow cytometry, gene expression will be analysed by qRTPCR and next generation sequencing, and protein analysis will be performed by proximity extension assays. In addition, alternative methods for the whole workflow will be covered in interactive sessions of lectures and Q&A with key experts in the field. Data collected by the different methods will be analysed during in-depth sessions, which are a strong component of the course.

Audience

This course targets young scientists entering the field of single cell analysis and wanting to learn about the benefits and limitations of the approach by performing a complete single cell analysis workflow.

Modules/Resources

• Single cell isolation with FACS
• Single-cell genomics using Strand-Seq
• Single-cell epigenetics using bisulfite sequencing
• Microdroplet-based single-cell transcriptomics
• Single cell NGS data analysis

Learning Outcomes

After finishing the course, attendees will have an overview of available methods in single cell omics and a basic overview of data analysis of single-cell NGS data sets (genomics, epigenetic and transcriptomics). Attendees will also be able to set up some methods in their lab with the knowledge of the detailed protocol and troubleshooting.

For an impression of the course check the 2017 course programme.

Speakers

Speakers & Trainers

Bianka Baying

EMBL Heidelberg

Germany

Vladimir Benes

EMBL Heidelberg

Germany

Lars Borm

Karolinska Institutet

Sweden

Raffaele Calogero

University of Turin

Italy

Stephen Clark

Babraham Institute

UK

Francesca Cordero

University of Torino

Italy

Qiaolin Deng

Karolinska Institute

Sweden

Eileen Furlong

EMBL Heidelberg

Germany

Caroline Gallant

Uppsala University

Sweden

Karen Grimes

EMBL Heidelberg

Germany

Orane Guillaume-Gentil

ETH Zürich

Switzerland

Patrick Hasenfeld

EMBL Heidelberg

Germany

Susanne Heck

NIHR Biomedical Research Centre

UK

Hoybin Jeong

EMBL Heidelberg

Germany

Davis McCarthy

The University of Melbourne & St Vincent’s Institute of Medical Research

Australia

Katharina Danielski

10x Genomics

Germany

Malte Paulsen

EMBL Heidelberg

Germany

Dinko Pavlinic

EMBL Heidelberg

Germany

David Porubsky

University of Washington

USA

Benjamin Raeder

EMBL Heidelberg

Germany

Ashley Sanders

EMBL Heidelberg

Germany

Daniel Schraivogel

EMBL Heidelberg

Germany

Anders Ståhlberg

Sahlgrenska Academy at University of Gothenburg

Sweden

Oliver Stegle

EMBL-EBI

UK

Sergio Triana

EMBL Heidelberg

Germany

Yvonne Yeboah

EMBL Heidelberg

Germany

Scientific Organisers

Qiaolin Deng

Karolinska Institute

Sweden

Jan Korbel

EMBL Heidelberg

Germany

Anders Ståhlberg

Sahlgrenska Academy at University of Gothenburg

Sweden

Elisabeth Zielonka

EMBL Heidelberg

Germany

Course Organisers

Lea Hohmann

EMBL Heidelberg

Germany

Programme