Time-resolved macromolecular serial crystallography

Course overview

The advent of serial crystallography at free electron laser sources and the following adaptation of these methods at 3rd and 4th generation synchrotrons have opened new possibilities to perform room temperature time relolved studies in macromolecular crystallography.

This practical course aims to teach participants know-how of time-resolved serial crystallography and to illustrate the entire pipeline from sample-preparation to a 3D molecular movie. Lectures and practical sessions will be delivered to explain how to prepare micro-crystals of biomolecules, how to use various sample delivery methods for serial crystallography, to design time-resolved experiments, to collect data, and to process serial crystallographic data.

This course aims to train the next generation of researchers in

i) sample preparation: Participants will be trained and taught in the best practice to obtain homogenous
microcrystals and in characterisation prior to loading using different sample delivery methods,
ii) crystal delivery methods and data collection at a 4th generation synchrotron serial crystallography beamline: The new ID29 and similar endstations at other sources, presents experimenral setup and data collection protocols that are unique in the synchrotron facilities. The training will include data collection with diffetent sample delivery methods as available to the user community,
iii) data reduction: Diffraction data are recorded from still microcrystals with large bandwidth high flux X-ray beams which requires the use of specifically developed and adapted software.
iv) analysis of the electron density map differences: To correctly interpret and model the electron density differences map to highlight struct8ral changes induced by pump-probe and ligand mixing experiments.

Warning

EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
EMBL will never call or email you to ask for your credit card details or to request a payment.
All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.