EMBO Practical Course
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The advent of serial crystallography at free electron laser sources and the following adaptation of these methods at 3rd and 4th generation synchrotrons have opened new possibilities to perform room temperature time relolved studies in macromolecular crystallography.
This practical course aims to teach participants know-how of time-resolved serial crystallography and to illustrate the entire pipeline from sample-preparation to a 3D molecular movie. Lectures and practical sessions will be delivered to explain how to prepare micro-crystals of biomolecules, how to use various sample delivery methods for serial crystallography, to design time-resolved experiments, to collect data, and to process serial crystallographic data.
This course aims to train the next generation of researchers in
i) sample preparation: Participants will be trained and taught in the best practice to obtain homogenous
microcrystals and in characterisation prior to loading using different sample delivery methods,
ii) crystal delivery methods and data collection at a 4th generation synchrotron serial crystallography beamline: The new ID29 and similar endstations at other sources, presents experimenral setup and data collection protocols that are unique in the synchrotron facilities. The training will include data collection with diffetent sample delivery methods as available to the user community,
iii) data reduction: Diffraction data are recorded from still microcrystals with large bandwidth high flux X-ray beams which requires the use of specifically developed and adapted software.
iv) analysis of the electron density map differences: To correctly interpret and model the electron density differences map to highlight struct8ral changes induced by pump-probe and ligand mixing experiments.
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