We are sorry to inform you that we have to cancel this course, and apologise for the inconvenience.
The goal of this course is to provide participants with both the theory and practical skills required to design, prepare, run and analyze high-throughput single cell transcriptomic experiments using the Chromium Single Cell Controller from 10x Genomics. We will begin with a general introduction on the topic, including the cell partitioning technology, fundamentals of NGS, and the library preparation workflow. The hands-on session starts with cell handling followed by the preparation of transcriptome libraries from single-cells with a focus on best practices. Finally, we will quantify and quality control the prepared libraries, before sequencing on a NextSeq instrument. The final day will be dedicated to single-cell data analysis with a mixture of theory and hands-on experience using the Loupe Cell Browser from 10x Genomics.
This course is directed towards biologists, scientists and technicians who want to apply single-cell transcriptome sequencing and learn the basics of library preparation. Knowledge of next generation sequencing technology and cell culture is desirable but not essential.
After this course you should be able to:
Date: 25 - 29 Jul 2022
EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme