Super-Resolution Microscopy

Overview

Course Overview

This six day advanced course on fluorescence super-resolution microscopy will cover the theory and practical application of current super-resolution microscopy techniques to biological questions. The participants will have sufficient time to use current super-resolution microscopes for Localisation Microscopy (e.g. dSTORM /GSDIM) and STED Microscopy on a variety of fixed and living samples prepared during the course. Invited guest researchers together with experts from the EMBL and Leica Microsystems will ensure that all aspects are covered in depth.

Audience

This course is aimed at researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level) with access to super-resolution microscopy equipment. The participants should have an immediate need to apply these techniques for their biological questions. The course will strongly focus on practical work and is therefore especially suited for people who rely on advanced microscopy for their research.

Modules/Resources

The course will cover the entire range of skills needed to apply super-resolution microscopy in biology. The following topics will be covered in lectures as well as in practical workshops:

• Sample preparation for super-resolution microscopy
• Multi-Colour 3D STED microscopy
• Multi-Colour 3D Localisation Microscopy
• Data analysis for Localisation Microscopy
• Deconvolution of STED images
• Super-Resolution Imaging of living specimen (STED / PAINT)

Learning Outcomes

At the end of the course, the participants will be able to plan an experiment using super-resolution microscopy, acquire high quality data at the microscope and analyse the images with state-of-the-art computational methods.

This course is organised in cooperation with Leica Microsystems.

Speakers

Speakers

Christian Eggeling

University of Oxford

UK

Jan Ellenberg

EMBL Heidelberg

Germany

Mike Heilemann

Goethe University Frankfurt

Germany

Jürgen Reymann

Leica Microsystems CMS GmbH

Germany

Luc Reymond

EPFL Lausanne

Switzerland

Davide Gambarotto

University of Geneva

Switzerland

Giuseppe Vicidomini

IIT Central Research Lab Genova

Italy

Rifka Vlijm

Max Planck Insitute for Medical Research

Germany

Kai Walter

Leica Microsystems CMS GmbH

Germany

Katrin Willig

MPI of Experimental Medicine

Germany

Speakers and Trainers

Francesca Bottanelli

FU Berlin

Germany

Susan Cox

King´s College London

UK

Jana Döhner

University of Zurich

Switzerland

Jens-Peter Gabriel

Leica Microsystems CMS GmbH

Germany

Marko Lampe

Marko Lampe

Germany

Sebastian Schnorrenberg

EMBL Heidelberg

Germany

Ulf Schwarz

Leica Microsystems CMS GmbH

Germany

David Unnersjö Jess

KTH Royal Institute of Technology

Sweden

Trainers

Philipp Hoess, EMBL Heidelberg, Germany

Yiming Li, EMBL Heidelberg, Germany

Ulf Matti, EMBL Heidelberg, Germany

Julia Roberti, Leica Microsystems CMS GmbH, Germany

Falk Schneider, University of Oxford, UK

Erdinc Sezgin, University of Oxford, UK

Christoph Spahn, Johann Wolfgang Goethe-University Frankfurt, Germany

Scientific Organisers

Marko Lampe

EMBL Heidelberg

Germany

Rainer Pepperkok

EMBL Heidelberg

Germany

Ulf Schwarz

Leica Microsystems CMS GmbH

Germany

Course Organisers

Tim Nürnberger

EMBL Heidelberg

Germany

Programme