Liquid Biopsies – Virtual

Overview

Course Overview

Liquid biopsies have the advantage of being largely non-invasive and providing a vast amount of information for the detection and monitoring of diseases such as cancer. This course will provide an overview in the several techniques to detect and analyse circulating tumour cells (CTCs), cell free tumor DNA (ctDNA), miRNA in liquid biopsies, cell free tumor RNA (ctRNA) and proteins. Participants will be introduced to all aspects ranging from proper sampling through error free sequencing to data analysis. Additional lectures will cover extracellular vesicles and clinical aspects using liquid biopsies, especially in the field of cancer diagnostics.

Please note that all participants are expected to engage in all sessions.

Audience

This course targets young scientists entering the field of liquid biopsy analysis and wanting to learn about the benefits and limitations of different approaches.

Modules/Resources

• Ultra-sensitive mutation detection using sequencing
• Single cell analysis in liquid biopsies
• Digital PCR applications in the liquid biopsy workflow
• Protein analysis in liquid biopsies
• miRNA analysis in liquid biopsies
• Hands-on data analysis and interpretations

Learning Outcomes

Participants will receive an overview of various workflows in liquid biopsies, from sample preparation to data analysis. The course provides and overview of the current methods. Whereby a strong focus will be on experimental design, best practices, pitfalls as well as dos and don’ts. After the course, participants should be able to assess the best methods for their application as well as design their experiments.

What past participants say about the course:

“The Liquid Biopsy course gave me plenty of new insights for different applications of the technique in cancer. I learned new techniques, discussed my project with top peers and met a lot of highly motivated young professionals. I incorporated the learned applications in my research and this really improved my current project.” – Aleksei Tikhonov, Engelhardt Institute of Molecular Biology, RAS, Russian Federation

Speakers, Trainers and Organisers

Speakers and Trainers

Jörg Bantin

BioRad,

Germany

Lei Chen

Uppsala University,

Sweden

Leonie de Visser

Roche Diagnostics International,

Switzerland

Anders Edsjö

Skåne University Hospital,

Sweden

Stefan Filges

Sahlgrenska Academy at University of Gothenburg,

Sweden

Amin Forootan

MultiD Analyses AB

Switzerland

Tony E. Godfrey

Boston University School of Medicine,

USA

An Hendrix

Ghent University

Belgium

Romy Kirsten

National Center for Tumor Diseases (NCT) Heidelberg,

Germany

Mikael Kubista

Institute of Biotechnology and TATAA Biocenter,

Czech Republic

Ulf Landegren

Uppsala University,

Sweden

Manuel Marceliano Luna Santa-María

Sahlgrenska Academy at University of Gothenburg,

Switzerland

Michael Pfaffl

Technical University of Munich,

Germany

Beata Ramasz

EMBL Heidelberg,

Germany

Anders Ståhlberg

Sahlgrenska Academy at University of Gothenburg,

Sweden

Valérie Taly

Paris Descartes University,

France

Thorsten Voss

QIAGEN,

Germany

Scientific Organisers

Vladimir Benes

EMBL Heidelberg,

Germany

Anders Ståhlberg

Sahlgrenska Academy at University of Gothenburg,

Sweden

Course Organiser

Lisa Trinh

EMBL Heidelberg

Germany

Programme