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In-situ CLEM at Room Temperature and in cryo – Course and Conference Office

EMBO Practical Course

In-situ CLEM at Room Temperature and in cryo

Overview

EMBL is committed to sharing research advances and sustaining scientific interaction throughout the coronavirus pandemic. We are delighted to announce that the course is going virtual and invite you to join us online.

Course Overview

Correlative Light and Electron Microscopy (CLEM) allows precise localisation of an event of interest within cells and tissues. During this course, we will focus on a specific method that has been developed at EMBL that enables the location of fluorescent proteins with high accuracy at the ultrastructural level. The course will cover both the theory and practicals of high precision CLEM, as well as opportunities to discuss their research with CLEM experts and receive practical and troubleshooting advice.

Audience

This course is intended for scientists with experience in EM who want to further develop their skills in high-accuracy correlated microscopy.

Modules/Resources

The course will combine lectures and hands-on practical work covering all steps in the work flow including cryogenic sample preparation, ultramicroscopy, image registration and electron tomography.

Learning Outcomes

Participants will gain hands-on experience with the CLEM methods and learn tips from the people who developed the methods.

Participant Commitment

The virtual course has associated pre-course work that needs to be completed before the start of the course. Bear in mind that completing this activity takes time, we recommend to take a look at the materials as soon as you’ve been notified the information is online. Completing this activity takes approximately 7 hours. 

The 5-day virtual course in September 2021 includes some live lectures, but will mostly be devoted to hands-on work on real data, in small groups of 4-5 participants. 

General notes: 

• The virtual course will start on Monday, 30 August, with an onboarding session and access to the pre-recorded lectures and pre-course study materials (exercises, assignments, etc.). It will continue on Monday, 6 September with the regular programme. 

• The virtual course includes recorded / live lectures, interactive discussion sessions, online practicals, hands-on exercises, student presentations and networking. • Full-time attendance is required for the course duration. 

• Selected participants will have access to the recorded talks 1 week before the start of the event. Please note that watching the recordings prior to the start of the course is required and will take approximately 7 hours. 

• Information on the live stream and access to the virtual course platform will be provided shortly before the start of the event. 

• Access to the recorded talks and the virtual course platform will be available for 2 weeks after the end of the event.

Speakers and Trainers

Pedro Machado

Pedro Machado

King’s College London

UK

Scientific Organisers

Course Organisers

Irena Provaznikova

EMBL Heidelberg

Germany

Preliminary Programme

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  • The virtual course will start on Monday 30 August with an onboarding session and access to the pre-recorded talks from invited speakers and trainers. The course will continue on Monday 6 September with the regular programme.
  • Participants will be required to view the pre-recorded talks (7 h) prior to the main start of the course on Monday 6 September. During the five-day course duration, full-time attendance is required.  

All times in the programme below are shown as the time in Europe (Berlin, Amsterdam, Paris).

To find out the equivalent time zone in your location, enter Berlin, the programme time and date along with your city into the Time Zone Converter.

Day 0 – Monday 30 August 2021
TimeSession
13:00 – 15:00Onboarding
Ori Avinoam – Weizmann Institute of Science, Israel
Lea Hohmann – EMBL Heidelberg, Germany
Rachel Mellwig – EMBL Heidelberg, Germany
Irena Provaznikova – EMBL Heidelberg, Germany
Paolo Ronchi – EMBL Heidelberg, Germany
Martin Schorb – EMBL Heidelberg, Germany
Aline Schnieder – EMBL Heidelberg, Germany

Day 1 – Monday 6 September 2021
TimeSession
12:45 – 13:00Overview of the day
Ori Avinoam – Weizmann Institute of Science, Israel
Lea Hohmann – EMBL Heidelberg, Germany
Rachel Mellwig – EMBL Heidelberg, Germany
Irena Provaznikova – EMBL Heidelberg, Germany
Paolo Ronchi – EMBL Heidelberg, Germany
Martin Schorb – EMBL Heidelberg, Germany
Aline Schnieder – EMBL Heidelberg, Germany
Elisabeth Zielonka – EMBL Heidelberg, Germany
13:00 – 13:15Welcome, house notes and course overview
13:15 – 13:45Icebreaker activity: Speakers and trainers
13:45 – 14:30Icebreaker activity: Participants part I
14:30 – 14:45Break
14:45 – 15:30Icebreaker activity: Participants part II
15:30 – 15:45Break
15:45 – 16:45Live Q&A session based on pre-recorded talk
Paul Verkade – University of Bristol, UK
Christopher Peddie – The Francis Crick Institute, UK
16:45 – 17:00Break
17:00 – 17:45Live Q&A session based on pre-recorded talk
Paolo Ronchi – EMBL Heidelberg, Germany
17:45 – 18:15Social programme: Speed networking

Day 2 – Tuesday 7 September 2021
TimeSession
12:45 – 13:00Overview of the day
Ori Avinoam – Weizmann Institute of Science, Israel
Lea Hohmann – EMBL Heidelberg, Germany
Rachel Mellwig – EMBL Heidelberg, Germany
Irena Provaznikova – EMBL Heidelberg, Germany
Paolo Ronchi – EMBL Heidelberg, Germany
Martin Schorb – EMBL Heidelberg, Germany
Aline Schnieder – EMBL Heidelberg, Germany
13:00 – 13:45Live Q&A session based on pre-recorded talk
Anna Sartori-Rupp – Institut Pasteur, France
13:45 – 14:00Break
14:00 – 14:45Live Q&A session based on pre-recorded talk
Ori Avinoam – Weizmann Institute of Science, Israel
14:45 – 15:15Break with pub quiz
15:15 – 16:00Interactive practical session
16:00 – 16:15Break
16:15 – 17:00Interactive practical session
17:00 – 17:15Break
17:15 – 19:00Interactive troubleshooting session
Pedro Machado – King’s College London, UK
Rachel Mellwig – EMBL Heidelberg, Germany
Viola Oorschot – EMBL Heidelberg, Germany
Paolo Ronchi – EMBL Heidelberg, Germany
 Break-out rooms

Day 3 – Wednesday 8 September 2021
TimeSession
12:45 – 13:00Overview of the day
Ori Avinoam – Weizmann Institute of Science, Israel
Lea Hohmann – EMBL Heidelberg, Germany
Rachel Mellwig – EMBL Heidelberg, Germany
Irena Provaznikova – EMBL Heidelberg, Germany
Paolo Ronchi – EMBL Heidelberg, Germany
Martin Schorb – EMBL Heidelberg, Germany
Aline Schnieder – EMBL Heidelberg, Germany
13:00 – 14:00Live Q&A session based on pre-recorded talk
Helmut Gnaegi – Diatome Ltd, Switzerland
14:15 – 15:15Interactive practical session
Helmut Gnaegi – Diatome Ltd, Switzerland
Viola Oorschot – EMBL Heidelberg, Germany
15:15 – 15:30Break
15:30 – 16:15Live Q&A session based on pre-recorded talk
Wanda Kukulski – University of Bern, Switzerland
Julia Mahamid – EMBL Heidelberg, Germany
16:15 – 17:30Interactive practical session
Julia Mahamid – EMBL Heidelberg, Germany
Ievgeniia Zagoriy – EMBL Heidelberg, Germany
17:30 – 17:45Break
17:45 – 18:30Social programme: Online Pictionary
Day 4 – Thursday 9 September 2021
TimeSession
12:45 – 13:00Overview of the day
Ori Avinoam – Weizmann Institute of Science, Israel
Lea Hohmann – EMBL Heidelberg, Germany
Rachel Mellwig – EMBL Heidelberg, Germany
Irena Provaznikova – EMBL Heidelberg, Germany
Paolo Ronchi – EMBL Heidelberg, Germany
Martin Schorb – EMBL Heidelberg, Germany
Aline Schnieder – EMBL Heidelberg, Germany
13:00 – 14:00Interactive practical session
Paolo Ronchi – EMBL Heidelberg, Germany
14:00 – 14:15Break
14:15 – 15:45Interactive practical session
Anna Sartori-Rupp – Institut Pasteur, France
15:45 – 16:00Break
16:00 – 17:00Interactive practical session
Martin Schorb – EMBL Heidelberg, Germany
17:00 – 17:15Break
17:15 – 19:00Interactive troubleshooting session
Pedro Machado – King’s College London, UK
Christopher Peddie – The Francis Crick Institute, UK
Day 5 – Friday 10 September 2021
TimeSession
12:45 – 13:00Overview of the day
Ori Avinoam – Weizmann Institute of Science, Israel
Lea Hohmann – EMBL Heidelberg, Germany
Rachel Mellwig – EMBL Heidelberg, Germany
Irena Provaznikova – EMBL Heidelberg, Germany
Paolo Ronchi – EMBL Heidelberg, Germany
Martin Schorb – EMBL Heidelberg, Germany
Aline Schnieder – EMBL Heidelberg, Germany
13:00 – 13:30Live Q&A session to pre-recorded talk
Perrine Paul-Gilloteaux – University of Nantes, France
Martin Schorb – EMBL Heidelberg, Germany
13:30 – 14:45Interactive practical session
Perrine Paul-Gilloteaux – University of Nantes, France
Martin Schorb – EMBL Heidelberg, Germany
14:45 – 15:00Break
15:00 – 15:30Live Q&A session to pre-recorded talk
Perrine Paul-Gilloteaux – University of Nantes, France
15:30 – 16:30Interactive practical session
Perrine Paul-Gilloteaux – University of Nantes, France
Martin Schorb – EMBL Heidelberg, Germany
17:00 – 17:15Break
17:15 – 18:00Interactive practical session
Perrine Paul-Gilloteaux – University of Nantes, France
Martin Schorb – EMBL Heidelberg, Germany
18:00 – 19:00Wrap-Up & Farewell

Sponsors

Event Supporter:

Sponsorship Opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Practical Information

Registration Fees and Motivation Letter

The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration Fees (include admission and all course materials):

Academia125 Euro
PhD Student125 Euro
Industry225 Euro

Confirmation and Payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation Letter Submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

Motivation letter length: 
The maximum limit of 2000 characters (ca. 200 words) refers to manually typed text (spaces included).

Free field text length: The maximum characters for your answers in the free field text boxes is 255 (spaces included).Text only: If you copy-paste the text, hidden formatting might still be included and you will be informed that your text exceeds the character limit. We recommend either:

  1. saving your text as text only in your editor or Email programme, OR
  2. copy-pasting it into Notepad and then onto the website.

Symbols: If you have special symbols in your text make sure you are using Unicode characters, otherwise these will not be recognised by the tool.

Further Details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial Assistance

Financial Assistance

Registration Fee Waivers

All academic and student registrants are invited to apply for a registration fee waiver, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO. The registration fee waiver covers the registration sum that you have paid to attend the meeting. Conference participants are not required to pre-pay the registration fee to be selected for a fee waiver for a virtual meeting. If you have already paid the registration fee and are awarded a fee waiver, it will be reimbursed after the meeting. Course participants are required to pay the course fee in advance, which will then be reimbursed after the recipient has attended the course.

Childcare Grants

For participants and speakers with childcare responsibilities there is the possibility to apply for a grant, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO, to offset childcare costs incurred when participating at a virtual event. Eligible costs include fees for a babysitter or childcare facility or travel costs for a care giver. Please note that priority will be given to early stage researchers. Costs will be reimbursed after the meeting only once a reimbursement form and original receipts have been received. Attendance at the event is required in order to be eligible to receive the reimbursement. In order to apply for this grant, you must be registered by the abstract submission deadline.

Application

Applications for financial assistance can be submitted via the submission portal* (for the submission of abstracts for conferences or the submission of motivation letters for courses) by completing the Financial Assistance Application Section (underneath the section for entering abstract/motivation letter information). The link to the portal can be found in the registration confirmation email that you will receive after registering for the conference or course.

For conferences, if you are not submitting an abstract, you can still apply for financial assistance in the submission portal. Read the instructions on how to apply for financial assistance.

Note that priority will be given to those submitting an abstract to present at the conference. In your application you will be asked to answer questions regarding your motivation for applying, and, for registration fee waivers, the reasons why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.

Selection

The scientific organisers will select the recipients of registration fee waivers during the abstract selection process for conferences and the participant selection process for courses. Results will be announced approximately 3 – 4 weeks before the event start date. Selection results do not impact your admission to the meeting. Registration fee waiver selection is based on your current work or study location, your motivation for applying, the reasons for needing financial support and the impact this event will have on your career. Childcare grants are allocated based on career stage, with priority given to early stage researchers.

Further details

Check out this list of external funding opportunities or get more information on attending the conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Technical Requirements

Below you will find the software and technical requirements for this course.

General

  • Headsets is highly recommended for improved voice clarity and better audio quality

Internet requirements

  • Client computer with fast and stable internet access for the live virtual sessions:
  • Minimum: 50 mbit download and 10 mbit upload
  • Preferred: 100 mbit download and upload (at your host institute)

Additional Software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.

Zoom will be used for the live talks and Q&A sessions. More information will be provided closer to the start of the course

Other Information

While using the software during the course, please make sure you have nothing else using your bandwidth. We recommend using a wired connection.

Virtual Participation Guidelines

Guidelines

Please do:

  • Use the event-specific hashtag as communicated during the event for any related tweets
  • Tweet unless the speaker specifically says otherwise
  • Be mindful of unpublished data
  • Be respectful in tone and content

Please don’t:

  • Share your login details to eCampus with others
  • Broadcast the conference to unregistered participants
  • Capture, transmit or redistribute data presented at the meeting unless presenter gives explicit consent
  • Use offensive language in your posts
  • Engage in rudeness or personal attacks

Additional information can be found in our Code of Conduct.

Health and well-being

It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in eCampus for you to enjoy during the event.

How to ask questions

Questions during and after the talks can be asked in the live streaming platform. If time runs out or you think of a question later, you can use the EMBL eCampus forum or send a direct message to the speaker/trainer.

Time zone

The programme is planned based on the Europe (Berlin, Amsterdam, Paris) time zone, unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accommodate as many time zones as possible when creating the programme. Please take your time zone into consideration when planning your attendance.

Date: 6 - 10 Sep 2021

Location: Virtual


Deadline(s):

Application: Closed


Contact: Lea Hohmann


EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

Corporate partners

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