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FISHing for RNAs: Classical to Single Molecule Approaches – Course and Conference Office

EMBO PRACTICAL COURSE

FISHing for RNAs: Classical to Single Molecule Approaches

Overview

EMBL is committed to sharing research advances and sustaining scientific interaction throughout the coronavirus pandemic. We are delighted to announce that the course is going virtual and invite you to join us online.

Course Overview

Over the past decades it became evident that a RNA transcripts specific amount and localisation are important in order to understand the biological function of its (non-) coding gene. Whereby state-of-the-art in situ hybridisation (ISH) technologies are key to visualise these RNAs in a given tissue or cell.

This course aims to highlight three powerful RNA visualisation techniques in histological tissue samples. During this course, the participants will learn to validate the cellular localisation of candidate genes from transcriptome studies through conventional ISH and, the more recent cutting-edge, smFISH (Single Molecule Fluorescent ISH) and HCR-ISH (Hybridization Chain Reaction), based RNA ISH hybridisation methods. Additionally, the course will also cover essential tissue embedding protocols that are adapted for use in visually interrogating RNA transcripts in mammalian tissues.

Audience

This course is directed to early stage scientists (PhD and Postdocs) from the fields of RNA and molecular biology as well as various fields with an interest in these methodologies.

Speakers and Trainers

Monica Ballarino

Sapienza University of Rome

Italy

Mainak Bose

EMBL Heidelberg

Germany

Marina Chekulaeva

Max Delbrück Center for Molecular Medicine

Germany

Sara Di Persio

Centre of Reproductive Medicine and Andrology – University Hospital Münster

Germany

Imre Gaspar

Institute of Molecular Biotechnology

Austria

Emerald Perlas

EMBL Rome

Italy

Ewa Piskadlo

Friedrich Miescher Institute for Biomedical Research

Switzerland

Tim Pollex

EMBL Heidelberg

Germany

Andre Schwarz

Max Planck Institute for Brain Research

Germany

Programme

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  • The virtual course will include interactive discussion sessions, online practicals, hands on exercises and virtual networking.
  • Participants will be required to view the pre-recorded talks prior to the main start of the course on Monday, 26 July 2021. During the five-day course duration, full-time attendance is required. 

The following times are used in the programme below:

  • Central European Summer Time (CEST): eg. Berlin, Amsterdam Paris

To find out the equivalent time zone in your location, enter Berlin, the CEST programme time and your city into the Time Zone Converter.

Day 1 – Monday, 26 July 2021
Time (CEST)Speaker
12:45 – 13:00Check-in and overview of the day
13:00- 13:15Welcome and house notes and course overview
13:15 – 14:30Icebreaker (e.g. flashtalks)
14:30 – 14:45Break
14:45 – 15:15Live Q&A session based on pre-recorded talk:
Histology overview and applications
Emerald Perlas – EMBL Rome, Italy
15:15 – 15:45Live Q&A session based on pre-recorded talk :
RNA detection methods
Imre Gaspar – Institute of Molecular Biotechnology, Austria
15:45 – 16:15Speed networking break
16:15 – 17:15Student presentations
Day 2 – Tuesday, 27 July 2021
Time (CEST)Speaker
12:45 – 13:00Check-in and overview of the day
13:00- 13:30Live Q&A Session based on pre-recorded talk
Differentially localised CDC42 mRNA isoforms
Marina Chekulaeva – Max Delbrück Center for Molecular Medicine, Germany
13:30- 14:15Live Q&A Session based on pre-recorded talk
Tom Pollex – EMBL Heidelberg, Germany
14:15-14:30Break
14:30- 15:00Live interactive practical:
Sample sectioning live lab demo
Emerald Perlas – EMBL Rome, Italy
15:00-15:30Break
15:30- 16:00Live interactive practical continues :
Sample sectioning tips and tricks
Emerald Perlas – EMBL Rome, Italy
16:00-17:00Student presentations
Day 3 – Wednesday, 28 July 2021
Time (CEST)Speaker
12:45 – 13:00Check-in and overview of the day
13:45- 14:15Interactive practical:
Imaging conventional ISH- live Q&A to pre-recorded video
Emerald Perlas – EMBL Rome, Italy
13:00- 13:45Live Q&A Session based on pre-recorded talk
Mainak Bose – EMBL Heidelberg, Germany
14:15-14:30Break
14:30- 15:00Interactive practical:
Introduction to Probe design- LIve Q&A to pre-course assignment
Imre Gaspar – Institute of Molecular Biotechnology, Austria
15:00-15:30Break
15:30- 16:00Interactive practical:
Introduction to Probe design- LIve Q&A to pre-course assignment
Imre Gaspar – Institute of Molecular Biotechnology, Austria
16:00-17:00Student presentations

Day 4 – Thursday, 29 July 2021
Time (CEST)Speaker
12:45 – 13:00Check-in and overview of the day
13:00- 13:45Live Q&A Session based on pre-recorded talk
Ewa Piskadlo – Friedrich Miescher Institute for Biomedical Research, Switzerland
13:45- 14:15Interactive practical:
Single Molecule Fluorescent ISH workflow- FAQs
Imre Gaspar – Institute of Molecular Biotechnology, Austria
14:15-14:30Break
14:30- 15:00Interactive practical :
Single Molecule Fluorescent ISH Imaging- Demo
Imre Gaspar – Institute of Molecular Biotechnology, Austria
15:00-15:30Break
15:30- 16:00Live practical: continues
Single Molecule Fluorescent ISH Imaging- Demo
Imre Gaspar – Institute of Molecular Biotechnology, Austria
16:00-17:00Student presentations
Day 5 – Friday, 30 July 2021
Time (CEST)Speaker
12:45 – 13:00Check-in and overview of the day
13:00-13:30Live Q&A Session based on pre-recorded talk or live session
ncRNA Charme in cardiac and skeletal muscle
Monica Ballarino – Sapienza University of Rome, Italy
13:30-14:15Live interactive discussion:
The solution Room:
Experts and peer supported advice on your most pressing problems
14:15:14:30Break
14:30- 15:30Interactive practical:
Basics ISH image analysis- on provided data set
15:30-16:00Break
16:00-17:00Interactive practical:
Basics ISH image analysis-on provided data set
17:00- 17:15Interactive Session: Course warp
up session

– take away message for particpants
– short feedback round
17:15Goodbye and end of course

Sponsors

Sponsorship Opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Date: 26 - 30 Jul 2021

Location: Virtual


Deadline(s):

Registration: Closed


Contact: Maryann Heck


EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

Corporate partners

Associate partners

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