Methods for Analysis of Circular RNAs: No Tautology

Overview

Course Overview

Circular RNAs (CircRNAs) are a rediscovered class of RNA species that originate from back-splicing events, which have been reported across all domains of life. Many circular RNAs are stable, abundant and conserved between species. They exhibit context-dependent expression patterns, which are often linked to disease states. Validated circular RNA functions are numerous and range from regulation of transcription and splicing to RNA/protein sponging and scaffolding. However, CircRNAs are by and large the most underexplored class of all non-coding RNAs. This course will meet the specific need to explore complexity, distribution, function and mechanisms of circRNA action experimentally and computationally.

The presented methods and approaches are widely applicable to all organisms beyond standard models. The practical part will include detection and quantification of circRNAs by low- and high-throughput methods, troubleshooting, data analysis and integration as well as computational assessment of circRNA candidates and function prediction.

Audience

This course is intended for PhD students and young Postdoctoral researchers.

Modules/Resources

• RNA splicing
• Computational Biology
• Gene expression
• RNA Localisation
• Quantitative Biology

Learning Outcomes

Participants will go through all wet-lab steps required for preparation of circRNA-seq libraries and will be introduced to basic bioinformatics required for analyses of circRNA-seq data.

Speakers

Speakers

Marie-Laure Baudet

University of Trento

Italy

Thomas Birkballe Hansen

Aarhus University

Denmark

Irene Bozzoni

Sapienza University of Rome

Italy

Christoph Dieterich

Heidelberg University Hospital

Germany

Stefanie Dimmeler

Goethe-University Frankfurt am Main,

Germany

Myriam Gorospe

National Institute on Aging

USA

Tobias Jakobi

Heidelberg University Hospital

Germany

Sebastian Kadener

Brandeis University

USA

Trainers

Nayara Trevisan Doimo de Azevedo

EMBL Heidelberg

Germany

Mirco Castoldi

Uniklinik RWTH Aachen

German

Alessio Colantoni

Sapienza University of Rome

Italy

Jessica Eschenbach

Heidelberg University Hospital

Germany

Tobias Jakobi

Heidelberg University Hospital

Germany

Linda Masante

University of Trento

Italy

Jelena Pistolic

EMBL Heidelberg

Germany

Scientific Organisers

Marie-Laure Baudet

University of Trento

Italy

Vladimir Benes

EMBL Heidelberg

Germany

Irene Bozzoni

Sapienza University of Rome

Italy

Christoph Dieterich

Heidelberg University Hospital

Germany

Course Organisers

Christina Dollt

EMBL Heidelberg,

Germany

Programme