SAXS experiments on macromolecules, nanoparticles and bio-nanoconjugates ranging from a few kDa to Gda.
High throughput data collection using an automated liquid handling sample changer using about 30 μl sample per measurement (in-vacuum setup).
A single frame exposure time is 50 to 100 msec (with a standard monochromator) or 1.2 msec (multilayer monochromator) leading to total exposures about 1-2 seconds depending on the capillary diameter (1 or 2 mm), flow rate, and susceptibility to radiation damage.
Size-exclusion (SEC-SAXS), ion-exchange and affinity chromatography SAXS (FPLC/HPLC – Agilent BioInert system with an autosampler).
Parallel SEC-SAXS measurements combined with on-line multi-angle laser light scattering (MALLS) and dynamic light scattering (Wyatt TREOS-QELS/T-Rex).
Wide-angle scattering (WAXS) setup up to s≈25nm-1 (nominal resolution 0.25nm).
Anomalous SAXS (6-20keV).
Time resolved measurements (sub-msec resolution with multilayer monochromator) and temperature scans.