The De Renzis group aims to understand how cells build tissues during organismal development, using a combination of quantitative imaging and optogenetics-based synthetic approaches.Edit
Tissue morphogenesis is triggered by shape changes in single cells or group of cells. This remodelling depends on a complex interaction between cortical forces exerted by the actin cytoskeleton and membrane homeostasis (i.e. vesicular trafficking and lipid metabolism). Using the early Drosophila embryo as model organism, we wish to understand how membrane trafficking and cytoskeletal dynamics are regulated during morphogenesis and how this, in turn, impacts on specific cell and tissue behaviour.
We have recently developed new optogenetic methods to manipulate key components of the membrane transport machinery and cytoskeletal regulators with cellular precision in situ during embryonic development (Figure 1). Using these novel tools in combination with two-photon microscopy we can now control protein function with high-spatio temporal precision and thus functionally link subcellular to supracellular processes during morphogenesis. We are also implementing optogenetics to study signalling systems and induce tissue-specific differentiation programmes at will.
Using a combination of imaging, genome engineering, optogenetic, and biochemical approaches, we wish to elucidate how machineries controlling intracellular trafficking re-organise during differentiation and how this in turn impacts on global changes in tissue morphology. One long-term goal of the group will be to reconstitute morphogenetic processes in naïve tissues using synthetic approaches.