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Building networks 2024: engineering in vascular biology – Course and Conference Office

EMBO Workshop

Building networks 2024: engineering in vascular biology

Overview

This conference will take place at EMBL Barcelona.

Conference overview

Blood vessels are essential for the formation and maintenance of larger tissues and organs. The vascular system regulates exchange of oxygen, nutrients and immune cells – the dysfunction of which can cause severe pathologies. Mouse models, genetics and histology of human samples, coupled with recent advances in imaging, single-cell approaches and computational modelling, have provided significant knowledge to the field of vascular biology. Yet, in vivo models are highly complex and often need to be combined with simpler in vitro studies to get mechanistic and molecular insights.

Growth of in vitro blood vessels presents numerous research challenges due to their complex 3D geometry, and a strong coupling between biology and mechanics.
Recent advances in microfluidic and 3D bioprinting offer a higher degree of control over design, system components and biological function.

With the emergence and consolidation of these technologies, together with human iPS cell technologies, the vascular biology field is starting to model vascularized tissues and human vascular diseases in vitro.

In this meeting, we intend to bring together vascular biologists and bioengineers from across the world to build research synergies to tackle long standing questions in the field of vascular biology.

Session topics

  • Developmental vascular biology
  • Vascular mechanobiology
  • Aging in vascular biology
  • Vascular integrity and thrombosis
  • Vascular signaling and angiogenesis
  • Lymphatics
  • Vascular cardiobiology and engineering

Speakers

Keynote Speakers

Speakers

Abdul Barakat

École Polytechnique

France

Maike Frye

University Medical Center Hamburg

Germany

Li-Kun Phng

RIKEN Center for Biosystems Dynamics Research

Japan

Scientific Organisers

Conference Organisers

Preliminary programme

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  • Programme is preliminary and subject to change.
  • For registered participants, recorded talks will be accessible on demand for 2 weeks after the end of the event, unless indicated otherwise.
Day 0 – Sunday, 5 May 2024
Time
(Europe/Barcelona)
Afternoon (time tbc)Informal city tour (optional)
Day 1 – Monday, 6 May 2024
Time
(Europe/Barcelona)
Speaker
09:00 – 09:45Registration and Welcome coffee
09:45 – 10:00Opening remarks by scientific organisers
10:00 – 11:00Keynote lecture: Engineering vasculature: How good is good enough?
Christopher Chen – Boston University, USA
Session 1 – Developmental vascular biology
11:00 – 11:30Talk title tba
Rui Benedito – CNIC, Spain
11:30 – 11:45Selected short talk #1
11:45 – 12:00Selected short talk #2
12:00 – 12:30Growing vasculature in engineering tissues
Kelly Stevens – University of Washington, USA
12:30 – 14:00Lunch
From 13:00 concurrent lab tour
Session 2 – Vascular mechanobiology
14:00 – 14:30Talk title tba
Maike Frye – University Medical Center Hamburg, Germany
14:30 – 14:45Selected short talk #3
14:45 – 15:00Selected short talk #4
15:00 – 15:30Mechanical regulation of vascular smooth muscle cell phenotypic switching in arterial disease
Thomas Iskratsch – Queen Mary University of London, UK
15:30 – 16:00Coffee break & Meet the speakers
16:00 – 16:30Light-based bioprinting for perfusable engineered tissues and vascular models
Riccardo Levato – Utrecht University, The Netherlands
16:30 – 16:45Selected short talk #5
16:45 – 17:00Selected short talk #6
17:00 – 17:30Combined forces of actin cytoskeleton and hydraulics in shaping blood vessels
Li-kun Phng – RIKEN Center for Biosystems Dynamics Research, Japan
17:30 – 18:00Flash talks (5 talks x 5 min)
18:00 – 19:45Poster Session 1 (odd numbers) with beer and snacks
Day 2 – Tuesday, 7 May 2024
Time
(Europe/Barcelona)
Speaker
07:00 – 08:00Group run (optional)
09:00 – 10:00Keynote lecture
Stefania Nicoli – Yale University, USA
Session 3 – Aging in vascular biology
10:00 – 10:30Vascular Aging: Causes and Consequences
Anjali Kusumbe – University of Oxford, UK
10:30 – 10:45Selected short talk #7
10:45 – 11:15In vivo optical dissection of brain capillary function
Andy Shih – Seattle Children’s, USA
11:15 – 11:45Coffee break & Meet the speakers
Session 4 – Vascular integrity and thrombosis
11:45 – 12:15Engineering Anatomical-Scale Microvasculature for Disease Modeling
Ying Zheng – University of Washington, USA
12:15 – 12:30Selected short talk #8
12:30 – 12:45Selected short talk #9
12:45 – 13:15In vitro blood vessels as platforms for studying vascular physiology and pathology
Abdul Barakat – École Polytechnique, France
13:15 – 14:45Lunch
From 13:45 concurrent lab tour
Session 5 – Vascular signaling and angiogenesis
14:45 – 15:15Talk title tba
Katie Bentley – The Francis Crick Institute, UK
15:15 – 15:30Selected short talk #10
15:30 – 15:45Spatiotemporal regulation of neurovascular patterning
Alexandre Dubrac – University of Montreal, Canada
15:45 – 16:15Coffee break & Meet the speakers
16:15 – 16:45Speaker tba
16:45 – 17:00Selected short talk #11
17:00 – 17:15Talk title tba
Shahin Rafii – Weill Cornell, USA
17:15 – 17:45Flash talks (5 talks x 5 min)
17:45 – 19:30Poster Session 2 (even numbers) with beer and snacks
19:30 – 22:00Conference dinner
Day 3 – Wednesday, 8 May 2024
Time
(Europe/Barcelona)
Speaker
09:00 – 10:00Keynote lecture: Engineering vascularized tissue flaps
Shulamit Levenberg – Technion – Israel Institute of Technology, Israel
Session 6 – Lymphatics
10:00 – 10:30Talk title tba
Stefan Schulte-Merker – WWU Münster, Germany
10:30 – 10:45Selected short talk #12
10:45 – 11:15Coffee break & Meet the speakers
11:15 – 11:45Developmental origins of blood and lymphatic endothelium
Oliver Stone – University of Oxford, UK
11:45 – 12:00Selected short talk #13
12:00 – 12:30Coupling shear stress responses and lymphatic vascular morphogenesis
Tatiana Petrova – University of Lausanne, Switzerland
12:30 – 14:00Lunch
From 13:00 concurrent lab tour
Session 7 – Vascular cardiobiology and engineering
14:00 – 14:30Tissue-specific in vitro vascular models to study development and disease
Kristina Haase – EMBL Barcelona, Spain
14:30 – 14:45Selected short talk #14
14:45 – 15:00Selected short talk #15
15:00 – 15:30Unlocking the Potential of Organoid and Tissue Models for Drug Discovery with Engineered Vasculatures
Boyang Zhang – McMaster University, Canada
15:30 – 16:00Microfluidic Vessels-on-chips for Disease Modeling
Andries van der Meer – University of Twente, The Netherlands
16:00 – 16:15Closing Remarks and Short talk & Poster Prize Awards

Practical information

Registration fees and abstract submission

On-site registration fees include admission, conference materials, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.

Virtual registration fees include access to all of the talks (livestreamed and on demand) and facility to submit questions.

On-site Academia€425
On-site PhD Student€325
On-site Industry€750
On-site Journal Editors*€250
Virtual Academia€200
Virtual PhD Student€150
Virtual Industry€275

* Editors from scientific journals are allowed to attend at a reduced rate, but asked to contribute to the conference in return by taking part in ‘meet the editors’ sessions or other planned activities within the programme.

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Accredited journalists may be eligible to register for complimentary press registration. Registrants may be required to provide accreditation or equivalent proof of press membership after registration. Please contact Aline Schnieder for more information. Please note that we do not offer complimentary registrations for editors of scientific journals.

Confirmation and payment

Registration will be on a first come, first served basis. Your place can only be confirmed after payment of the registration fee. If you are added to our waiting list, please consider taking advantage of our offerings to participate virtually.

On-site participants: Types of payments accepted are international bank transfers and credit card payments. 

Virtual participants: We are only able to accept card payments. In exceptional cases we can accept bank transfers. Please contact events@embl.de for details.

Abstract submission

Only participants registering to attend the on-site event are eligible to submit an abstract. Abstracts will not be accepted from virtual participants.

After registration you can submit your abstract via a separate link that will be provided in the email confirmation. Alternatively, you can access the link on the confirmation page directly after registering. The same login credentials are used for both processes.

Please note:

Abstract body: The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).

If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.

If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

Title: The title should not exceed 20 words. Only the first word of the title should start with a capital letter and the rest should be lowercase.

Authors and affiliations: Please fill in the author’s details as requested in the online form. The compulsory fields are: First Name, Last Name, Organisation Name (Affiliation or Company), Country and Email.

Kindly mark only one author in the role of First Author and please don’t forget to indicate who will be the Presenter.

Please enter your co-authors correctly via the system by adding accounts together with their organisation/institute. Do not copy-paste them into the body of the abstract text, as they will not be indexed in the abstract book.

Presentation types: When submitting your abstract, you can apply for an oral or poster presentation. A selection process will take place with the results announced 2-3 weeks after the abstract submission deadline.

For detailed instructions on how to submit a conference abstract, follow the instructions provided in this video.

Please check our FAQs pages for further information on how to submit an abstract.

Financial assistance

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants, and childcare grants.

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course or conference.

Travel grant

The travel grant will cover the cost of travel to an on-site event (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

–up to €400 for participants travelling to an EMBL Conference or EMBO|EMBL Symposium from within Europe.

–up to €1000 for participants travelling to an EMBL Conference or EMBO|EMBL Symposium from outside Europe.

–up to €500 for any participant travelling to an EMBO Workshop.

–up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Workshop.

–up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Workshop.

*Registration fees are only covered for EMBO Workshops

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, and organisers when attending a conference.

Eligible costs include (but are not limited to) fees for a baby-sitter or child-care facility, and travel costs for a caregiver. Please note that priority will be given to early stage researchers. In order to apply for this grant, you must be registered by the abstract submission deadline. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Application

On-site participants

You may apply for financial assistance when submitting your abstract. In your application you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

Virtual participants

If you are attending virtually, you can apply for financial assistance in the submission portal by the abstract deadline. Read the instructions on how to apply for financial assistance. Only submissions for financial assistance will be accepted. Presentation abstracts cannot be submitted here and will be declined. 

In your application you will be asked to summarise your current work,  answer questions regarding why your lab cannot fund your attendance, and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

Selection

The scientific organisers will select the recipients of registration fee waivers and travel grants during the motivation letter or abstract selection process. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

View our list of external funding opportunities and information on attending a conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Venue

EMBL Barcelona is located at the Barcelona Biomedical Research Park (PRBB). The PRBB is located on Barceloneta seafront between Hospital del Mar and the Arts Hotel in the Olympic Port of Barcelona.

Address: Barcelona Biomedical Research Park, C/ Doctor Aiguader, 88, 08003 Barcelona

The conference is taking place in the PRBB auditorium. It is located on a raised level, in the heart of the PRBB building overlooking the sea, with independent access from the rest of the building. Access to the Auditorium will be by the stairs located at the entrance of the inner square of the building. For people with limited mobility, the access through lifts up the 1st floor can be used.

While on campus, please wear your conference badge at all times for security purposes.

PRBB, EMBL Barcelona, PhotoCredit: Jordi Casañas/EMBL
Accommodation

Accommodation is not included in the conference registration fee.

A list of hotels that are located near the conference venue will be added here shortly.

Please note that a city tax per person/per night for the stay needs to be paid (whether you book in a hotel or through Airbnb).

On-site information

Catering

Lunches and coffee breaks are included in the registration fee. The lunch buffets and coffee breaks will be set-up in front of the Auditorium. Snacks will be served on the first evening of the workshop during the Welcome reception (PRBB terrace), as well as during the poster sessions (groundfloor beneath the Auditorium). 

Dinners are at the participants own expense unless otherwise specified.  

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the PRBB auditorium.

Electricity and charging station

The mains voltage in Barcelona is 230 volts AC. The flat European standard and the Schuko plug CEE 7/7 fit into the Spanish sockets. Most Europeans do not need to buy an adapter plug for Spain. Please note that there are no power outlets in the auditorium. You can recharge your electronic devices at the reception area. We kindly ask you to bring your own charger cables. The charging station will be left unattended.           

Health and safety notes

Please read EMBL Barcelona’s COVID-19 terms and conditions for events.
Smoking is prohibited in all PRBB facilities.
Eating and drinking is prohibited in the Auditorium.
Do not enter any restricted areas or the laboratories unless authorised to do so. Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

  • Assist the casualty and provide first aid if trained to do so. 
  • Take to Hospital del Mar, Pg Maritim Barceloneta, 23*31 OR
  • Dial the emergency number 1020 from any internal phone OR
  • Dial 112
  • Please report all accidents to the Conference Officer at the Registration Desk.

In case of fire …

  • Press the nearest fire alarm button or dial the emergency number 1020 from any internal phone.
  • There will be a loudspeaker announcement (in Catalan, Spanish, English) if an evacuation is required. 
  • On hearing the evacuation message leave the building promptly following the escape route and exit signs and proceed to the meeting point (“Punto the reunion”) on Passeig Maritim Barceloneta next to the sea side.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor.

Internet

It is easy to access the internet via a wireless connection in any area of the building. When connecting a mobile device, the operating system automatically recognises the network “PRBB”. The password for this network is “darwin1809”.

The PRBB also participates in the Eduroam project, which aims to allow network access for visitors from academic institutions associated with Eduroam. Access to the service is always delivered with the same credentials as in the home institution.

Lost and found

“Lost and Found” items are kept at Registration and will be stored at the EMBL Barcelona reception for a short whileafter the EMBO workshop.

Luggage

There are no lockers available on-site to store your belongings. It is highly recommended to leave any valuables in your accommodation. An unguarded wardrobe area is behind the registration desk. You may use this wardrobe at the last day of the Workshop to store your luggage. 

The venue is directly located at the beachside. Be aware of your surroundings and belongings when leaving the building. 

Phone numbers

In case of emergency, contact Security on the ground floor: 

Ext. 1020 / 0034933160020 

Reception: Ext. 1000 or 0034933160000

Below are the most important emergency service phone numbers for Barcelona. 

Fire service: 080

Medical emergencies: 061

Police (Mossos d’Esquadra): 088

The Mossos d’Esquadra are Catalonia’s autonomous community police force. They are responsible for crime investigations, crime prevention and assistance at accident scenes. 

Guàrdia Urbana: 092 

The Guàrdia Urbana are responsible for public order by patrollling the roads and streets. They also look after traffic safety and control. The Guardia Urbana are also the first point of call for everyday situations such as neighbourhood disputes like noise etc.

All general emergencies: 112

If you become ill during the event, please notify the Conference Officer(s) immediately.  

Photography

During the conference an EMBL Photographer may be taking photographs. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Pickpockets

It is highly recommended to leave any valuables in your accommodation. The venue is located at the beachside which is a very busy area. Be aware of your surroundings and belongings when leaving the building.

Sightseeing

A variety of activities in Barcelona can be found in this website: https://www.barcelona-tourist-guide.com/

Time

Spain operates on Central European time (CET), which means that the country is 6 hours ahead of eastern standard time (EST) in the United States and 1 hour ahead of Greenwich mean time (GMT).

Travel to and from the venue

Our EMBO Workshop partner hotel, the “H10 Marina”, is a 15 minutes walk to the venue. 

If you prefer, you can also take the bus. The next bus stop is in 6 minutes walking distance to the hotel. Line V27 is departing from Av Icària – Joan Miró to Pg Marítim – Hospital del Mar. From there it is another 3 minute walk. 

Additionally, a taxi stand is located just outside the hotel main entrance.

Hotel address: Avinguda del Bogatell, 64-68 Olympic Village, 08005 Barcelona

Useful Spanish expressions

EnglishSpanish
HelloHola
Good morning/ Good afternoon/ Good evening / Good nightBuenos días/ Buenas tardes/ Buenas noches/ Buenas noches
I’m sorryLo siento / Perdona
Excuse me…Perdóneme…
How are you?¿Cómo estás?
I’m fine thanks. And you?Estoy bien, gracias. ¿Y usted?
What is your name?¿Cómo te llamas?
My name isMe llamo
Do you speak English?Habla usted Inglés?
I don’t understandNo entiendo
Please speak more slowlyPor favor hable más despacio
Thank youGracias
Where is the toilet?¿Dónde está el servicio?
Please call me a taxiPuedes avisar/llamar un taxi, por favor?
How do I get to….?Como llego a….?
A beer/two beers please Una cerveza / dos cervezas por favor.
A glass of red/white wine pleaseUn vaso de vino tinto / blanco por favor.
The menu, please.El menú, por favor.
Is there a local speciality?¿Hay alguna especialidad local?
I’m VegetarianSoy vegetariano, -a
It was deliciousEstaba delicioso
The bill, pleaseLa cuenta, por favor
I have a headacheMe duele la cabeza
I have a sore throatTengo dolor de garganta
My stomach hurtsMe duele el estómago
I’m allergic toSoy alérgico a
I need a doctor who speaks EnglishNecesito un médico que hable inglés
Transport information

Getting to Barcelona – By Plane

Barcelona-El Prat airport is located in El Prat de Llobregat just 12 km South West of the Barcelona city centre. It has two passenger terminals: Terminal 1 (T1) and Terminal 2 (T2). The airport has allocated airlines to terminals, regardless of the destination of the flight.

Airport Transfers to the city centre

The Aerobús is an express bus service that connects the Barcelona-El Prat airport with the city centre (Plaça Catalunya) in 35 minutes. You can take the Aerobús outside Terminal 1 (A1) and Terminal 2 (A2). This service runs every day of the year with departures between 5 and 15 minutes. From the city centre, you can take the metro or a bus to move around Barcelona.

The train service (R2 line) runs approximately every 30 minutes to and from Barcelona airport to the city centre. The journey takes 25 minutes. The train service operates from Terminal 2 (T2). To get from T2 to T1, use the shuttle bus service (Bus transit T1-T2). If you travel from the airport to the city centre, you can get off at Barcelona-Sants, Passeig de Gràcia or Clot which are serviced by metro stops.

You can also take the metro line L9 (orange) from the airport. It departs at Terminal 1 and connects at stations Torrassa (L1 and L10 Sud), Collblanc (L5 and L10 Sud) with metros to the city centre. 

You will find a taxi rank outside any of the main terminal exits. Look for the sign pointing to the nearest taxi rank. All official Barcelona taxis are black and yellow and are required to charge you only the value on the taximeter (plus any surcharge, e.g. luggage or night). The rates and surcharges are displayed inside the cab. In Barcelona, a taxi may be either ordered by phone, found at authorised taxi rank or flagged down in the street.            

Getting to Barcelona – By train

Barcelona Sants is the main train station for national and international arrivals and departures in Barcelona. It is situated in plaça dels Països Catalanes – Barcelona. National and regional trains also depart from França station. It is located at avinguda Marquès de l’Argentera, s/n – Barcelona.

Spanish Railways (RENFE)

Public Transport in Barcelona

Metro and tram

The metro network of Barcelona is very good and the trains are frequent and quick. You do not have to worry about timetables. Almost all attractions are within walking distance of a metro or tram station. When there are no subways, the gap can be filled by tram.

You can find the operating hours of the metro and tram here.

Buses

The operating times of the buses are different for each line. You will find a notice board with the timetable at each stop. They operate from about 5:00 clock to about 22:00 clock.

Night bus (NitBus)

Both the metro and the buses do not run all night. In Barcelona, there is a fairly dense network of night buses to get you back to your hotel all night long. Except for the line N0 all lines stop at Plaça Catalunya and can change there. The N0 line is a circular line that allows you to circle around the city center and change to the other NitBus. The night buses are operated by the private operator AMB, which is why the Barcelona Card and Hola BCN Ticket are not valid.

The N17 line goes to the airport.

The operating hours of the NitBus are different for each line, but they run from about 22:00 to 05:00 clock.

Sponsors

Media partners

EMBO Journal, an EMBO Press Journal

International Union of Biochemistry and Molecular Biology

Medinformatics, a Bon View Publishing journal

Open Biology, a Royal Society journal

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information on sponsoring possibilities.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Warning

EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

  • EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
  • EMBL will never call or email you to ask for your credit card details or to request a payment.
  • All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsoring@embl.de.

Media kit

Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.

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Date: 6 - 8 May 2024

Location: EMBL Barcelona

Venue: Barcelona Biomedical Research Park (PRBB)


Deadline(s):

Abstract submission: Closed

Registration (On-site): 25 Mar 2024

Registration (Virtual): 29 Apr 2024


Organisers:

  • Maria Bernabeu
    EMBL BARCELONA, SPAIN
    • Claudio Franco
      University of Lisbon, Portugal

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