Location: Room 53
System Specifications
- Upright fluorescence microscope system combined with a Laser Microdissector
- Five color excitation filters
- Suitable for fluorophores from DAPI to Far Red and transmitted image (Brightfield and Phase Contrast).
- Colored digital camera CD310 (1.3 megapixel)
- Black and White digital camera CD345
- Suitable as a laser microdissector for cells and tissue (UV laser).
- Suitable for imaging fixed specimen (no environmental chamber).
Microscope
Leica DMI 6000 CS AFC Bino inverted microscope with motorized XY-stage
Objectives
| Magnification | NA | Type | Immersion | Coverslip |
|---|---|---|---|---|
| 6.3x | 0.13 | For Microdissection | ||
| 10x | 0.3 | HC PL Fluotar | Dry | – / 0.17 |
| 20x | 0.4 | HCX PL Fluotar | Dry | Correction |
| 40x | 0.6 | HCX PL Fluotar | Dry | Correction |
| 63x | 1.25 | HC PL Apo | Oil | 0.17 |
| 100x | 1.32 | HC PL Apo | Oil | 0.17 |
Fluorescent source
Leica EL6000External light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours)
Fluorescence filter sets
| Name | Excitation filter | Emission filter | Dichroic |
|---|---|---|---|
| A | BP 340 – 380 nm | LP 430 nm | 400 nm |
| CFP | BP 436/20 nm | LP 515 nm | 455 nm |
| L5 | BP 480 – 656 nm | LP 590 nm | 580 nm |
| YFP | BP 500/20 nm | BP 535/30 nm | 515 nm |
| N2.1 | BP 515 – 560 nm | LP 590 nm | 580 nm |
| N3 | BP 546-12 | BP 600/40 | 565 |
| Y5 | BP 620/60 nm | BP 700/75 nm | 660 nm |
| LMD | Combined for Microdissection |
Softwares
Leica AF6000 64bit Z control and SW Autofocus, multichannel acquisition, stitching. Leica Microdissection software
Cameras
- Leica DFC345 FX C mount 0.55 x HC
- Leica DFC 450 C mount 0.55 x HC. CCD 2/3″ da 2560×1920 pixel
Power up and down procedure
- Please contact Alvaro Crevenna to schedule a session
- Report problems on the Log file
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