Depending on the imaging technique, this can be done by attaching fluorescent reporter molecules to them, or
by adding compounds to increase the contrast. For many imaging experiments to work, molecules or subcellular
structures have to be fixed in space. Adding chemical glues, letting molecules form a regular crystal, or
cooling down the sample to very low temperatures are the most common approaches.
Other experiments, on the contrary, require cells and molecules to be able to move around freely. This helps
biologists understand how cells develop over time, how tissues form, and how proteins shuttle between
different cellular compartments to perform their function.
The technology and experimental approaches in biological imaging are constantly being refined. This allows
for higher resolutions, faster imaging times, better contrasts – in sum, sharper and more detailed images
and videos of biological structures and processes. At EMBL, numerous researchers from multiple disciplines
are involved in advancing imaging techniques.
The atomic structures of molecules are revealed at EMBL Hamburg and Grenoble, where scientists use
high-energy radiation in synchrotrons, electron lasers, and X-ray beamlines to visualize crystallized
proteins. At EMBL Heidelberg and Rome, electron microscopy and advanced light microscopy approaches are
developed and applied. These techniques allow researchers to determine the shapes of proteins and protein
complexes, follow in real-time how cellular structures change upon external stimuli, or investigate how
cells develop into more complex biological structures.
The Heidelberg campus is currently expanded with a brand-new imaging facility to further foster these
activities. At EMBL Barcelona, entire biological structures such as tissues and organs can be visualized
through ‘mesoscopic imaging’. This unique combination of state-of-the-art imaging technologies enables
researchers at EMBL to visualize biology across multiple scales.
