This facility offers a collection of state-of-the-art light microscopy equipment and image processing tools.

Microscope:
Leica SP8 DLS
Location:
Building 13, room 509C
Usage:
Optical sectioning (CLSM), multi-tiling, FRAP, lighsheet, Navigator
Lasers:
405 nm, 488 nm, 552 nm, 638 nm
Detection:
2x PMT, 2x HyD; spectral range 400 – 800 nm
Filters:
DAPI, GFP, RFP (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Leica SP8 X STED 3D
Location:
Building 13, room 516B
Usage:
Stimulated emission depletion, Optical sectioning (CLSM), multi-tiling, FRAP, Navigator
Lasers:
405 nm, Argon Laser, WLL (470 – 670 nm) + 592 nm, 660 nm and 775 nm STED lasers
Detection:
2x PMT, 2x HyD; spectral range 400 – 800 nm
Filters:
DAPI, FITC, RFP (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Leica Stellaris 8
Location:
Building 13, room 514D
Usage:
TauSense, Optical sectioning (CLSM), multi-tiling, Navigator
Lasers:
405 nm, White light laser (440 – 790 nm)
Detection:
4x HyD S; spectral range 410 – 850 nm
Filters:
DAPI, GFP, RFP (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Nikon A1R HD25
Location:
Building 13, room 515B
Usage:
Optical sectioning (CLSM), multi-tiling, JOBS module, feedback microscopy
Lasers:
405 nm, 488 nm, 561 nm, 640 nm
Detection:
4x GaAsP, galvo + resonant scanner; spectral range 400 – 750 nm
Filters:
DAPI, GFP, mCherry/Cy5, CFP/YFP (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Olympus FV3000
Location:
Building 13, room 514E
Usage:
Optical sectioning (CLSM), multi-tiling
Lasers:
405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm
Detection:
4x GaAsP, galvo + resonant scanner; spectral range 400 – 800 nm
Filters:
DAPI, GFP, mCherry/Cy5, CFP/YFP (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Olympus FV3000 + RappOpto UGA-42
Location:
Building 13, room 512
Usage:
Optical sectioning (CLSM), multi-tiling, FRAP, optogenetics, photo-crosslinking, laser nano-surgery, ablation, uncaging, DNA damaging
Lasers:
405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm, pulsed for ablation 355 nm and 375 nm
Detection:
4x GaAsP, RappOptoelectronics UGA-42 scanner; spectral range 400 – 800 nm
Filters:
DAPI, GFP, RFP (eyepieces)
Incubator:
No
Contact person:

Microscope:
Zeiss LSM 780 NLO
Location:
Building 13, room 516D
Usage:
Multi-photon, Second harmonics generation (SHG), Optical sectioning (CLSM), photo activation, FRAP, FCS, FRET, uncaging, laser nano-surgery
Lasers:
405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594 nm, 633 nm, pulsed 690-1064 nm
Detection:
2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm
Filters:
DAPI, GFP, Ds-Red (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Zeiss LSM 880 AIRY fast
Location:
Building 13, room 516D
Usage:
Airy scan, Optical sectioning (CLSM), multi-tiling, FCS, feedback microscopy
Lasers:
405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
Detection:
2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm
Filters:
DAPI, GFP, Ds-Red (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Zeiss LSM 980 AIRY fast
Location:
Building 13, room 516C
Usage:
Airy scan, Optical sectioning (CLSM), multi-tiling, FCS, feedback microscopy
Lasers:
405 nm, 488 nm, 561 nm, 639 nm
Detection:
2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm, Axiocam 506 mono
Filters:
DAPI, GFP, Cy3, Penta (eyepieces + camera)
Incubator:
Yes
Contact person:

Microscope:
Zeiss LSM 900
Location:
Building 13, room 515A
Usage:
Optical sectioning (CLSM), multi-tiling, feedback microscopy
Lasers:
405 nm, 488 nm, 561 nm, 640 nm
Detection:
3x GaAsP; spectral range 405 – 750 nm, Axiocam 503 mono
Filters:
DAPI, FITC, TRITC, Cy5, Quad (eyepieces + camera)
Incubator:
Yes
Contact person:

Microscope:
Nikon Ti-2
Location:
Building 13, room 508E
Usage:
Color- and monochromatic imaging, multi-tiling, JOBS module, feedback microscopy
Fluo lamp:
LUMENCOR SPECTRA III
Detection:
Hamamatsu Orca Fusion CMOS mono + DS-Ri2 color
Filters:
GFP, mCherry, Penta (camera)
Incubator:
No
Contact person:

Microscope:
Nikon Ti-E
Location:
Building 13, room 514A
Usage:
Monochromatic imaging, multi-tiling, JOBS module, feedback microscopy
Fluo lamp:
LUMENCOR SPECTRA X
Detection:
Hamamatsu Orca Flash 4 V2 mono
Filters:
DAPI, GFP, Cy3, mCherry, Cy5 (camera)
Incubator:
Yes
Contact person:

Microscope:
Nanolive 3D cell Explorer Fluo
Location:
Building 13, room 509B
Usage:
Holotomographic imaging, Label-free imaging
Fluo lamp:
CooLED pE300
Detection:
Camera based
Filters:
DAPI-FITC-TRITC/Cy5 (camera)
Incubator:
Yes
Contact person:

Microscope:
Zeiss Microbeam (LMD)
Location:
Building 13, room 508D
Usage:
Laser micro-dissection
Fluo lamp:
Pulsed 355 nm laser, HXP 120 C metal halide lamp
Detection:
Axiocam 506 mono
Filters:
DAPI, GFP, RFP (camera)
Incubator:
No
Contact person:

Microscope:
Olympus iXplore SPIN SR
Location:
Building 13, room 515C
Usage:
High speed confocal and super resolution (SoRA), Dual-cam imaging
Laser:
405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
Detection:
2x ORCA-FLASH4.0 V3 mono
Filters:
DAPI, CFP, FITC, YFP, Cy3, mCherry, Cy5 (camera)
Incubator:
Yes
Contact person:

Microscope:
Nikon Ti-2 CSU W1 SORA Spinning disk
Location:
Building 13, room 508F
Usage:
High speed confocal and super resolution (SoRA), Dual-cam imaging
Laser:
405 nm, 488 nm, 561 nm, 640 nm
Detection:
2x Hamamatsu Orca FusionBT mono
Filters:
tba
Incubator:
Yes
Contact person:

Microscope:
Molecular Devices IXM
Location:
Building 13, room 508B
Usage:
Imaging multi-well plates and cell cultures
Fluo lamp:
Solid state light source
Detection:
Andor Zyla mono
Filters:
Hoechst, GFP, Cy3, TxRed, Cy5 (camera)
Incubator:
Yes (temperature only)
Contact person:

Microscope:
Nikon Ti-E Plateloader
Location:
Building 13, room 508B
Usage:
Equipped with plate loading robot for 20 plates, JOBS module
Fluo lamp:
Lumencor Sola SE II 365
Detection:
DS-Qi2 mono
Filters:
DAPI, FITC, Cy3, mCherry, Cy5 (camera)
Incubator:
No
Contact person:

Microscope:
Olympus ScanR IX83
Location:
Building 13, room 508B
Usage:
High throughput
Fluo lamp:
LED lamp from CoolLED
Detection:
Hamamatsu ORCA-Flash4.0 VS mono
Filters:
DAPI, FITC, mCherry, Cy5 (camera)
Incubator:
Yes
Contact person:

Microscope:
Zeiss LSM 900
Location:
Building 13, room 515A
Usage:
Optical sectioning (CLSM), multi-tiling, feedback microscopy
Lasers:
405 nm, 488 nm, 561 nm, 640 nm
Detection:
3x GaAsP; spectral range 405 – 750 nm, Axiocam 503 mono
Filters:
DAPI, FITC, TRITC, Cy5, Quad (eyepieces + camera)
Incubator:
Yes
Contact person:

Microscope:
Leica SP8 DLS
Location:
Building 13, room 509C
Usage:
Lighsheet (SPIM)
Lasers:
405 nm, 488 nm, 552 nm, 638 nm
Detection:
DLS: Hamamatsu Orca Flash
Filters:
DAPI, FITC, Quad (for lighsheet)
Incubator:
Yes
Contact person:

Microscope:
Luxendo MuVi SPIM
Location:
Building 13, room 509B
Usage:
Lighsheet (SPIM)
Lasers:
445 nm, 488 nm, 515 nm, 561 nm, 642 nm
Detection:
2x Hamamatsu orca flash 4 mono
Filters:
CFP, FITC/GFP, YFP, mCherry, Cy5 (camera)
Incubator:
Yes
Contact person:

Microscope:
Abbelight Olympus Safe360 (TIRF, SMLM)
Location:
Building 13, room 509A
Usage:
TIRF, HiLo, SMLM
Lasers:
405 nm, 488 nm, 530 nm, 640 nm
Detection:
2x Hamamatsu Orca Fusion mono
Filters:
Cy3 / Cy5, 560 nm BS (camera)
Incubator:
No
Contact person:

Microscope:
Leica SP8 X STED 3D
Location:
Building 13, room 516B
Usage:
Stimulated emission depletion, Optical sectioning (CLSM), multi-tiling, FRAP, Navigator
Lasers:
405 nm, 458 nm, 476 nm, 488 nm, 496 nm, 514 nm, WLL (pulsed 470 – 670 nm); STED lasers 592 nm, 660 nm and pulsed 775 nm
Detection:
2x PMT, 2x HyD; spectral range 400 – 800 nm
Filters:
DAPI, FITC, RFP (eyepieces)
Incubator:
Yes
Contact person:

Microscope:
Zeiss LSM 780 NLO
Location:
Building 13, room 516D
Usage:
Multi-photon, Second harmonics generation (SHG), Optical sectioning (CLSM), photo activation, FRAP, FCS, FRET, uncaging, laser nano-surgery
Lasers:
405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594 nm, 633 nm, pulsed 690-1064 nm
Detection:
2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm
Filters:
DAPI, GFP, Ds-Red (eyepieces)
Incubator:
Yes
Contact person: