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Advanced Light Microscopy Facility

This facility offers a collection of state-of-the-art light microscopy equipment and image processing tools.

Equipment

Point Scanner


Microscope:

Leica SP8 DLS

Location:

Building 13, room 509C

Usage:

Optical sectioning (CLSM), multi-tiling, FRAP, lighsheet, Navigator

Lasers:

405 nm, 488 nm, 552 nm, 638 nm

Detection:

2x PMT, 2x HyD; spectral range 400 – 800 nm

Filters:

DAPI, GFP, RFP (eyepieces)

Incubator:

Yes

Link:


Microscope:

Leica SP8 X STED 3D

Location:

Building 13, room 516B

Usage:

Stimulated emission depletion, Optical sectioning (CLSM), multi-tiling, FRAP, Navigator

Lasers:

405 nm, Argon Laser, WLL (470 – 670 nm) + 592 nm, 660 nm and 775 nm STED lasers

Detection:

2x PMT, 2x HyD; spectral range 400 – 800 nm

Filters:

DAPI, FITC, RFP (eyepieces)

Incubator:

Yes

Contact person:

Link:


Microscope:

Leica Stellaris 8

Location:

Building 13, room 514D

Usage:

TauSense, Optical sectioning (CLSM), multi-tiling, Navigator

Lasers:

405 nm, White light laser (440 – 790 nm)

Detection:

4x HyD S; spectral range 410 – 850 nm

Filters:

DAPI, GFP, RFP (eyepieces)

Incubator:

Yes

Contact person:

Link:


Microscope:

Nikon A1R HD25

Location:

Building 13, room 515B

Usage:

Optical sectioning (CLSM), multi-tiling, JOBS module, feedback microscopy

Lasers:

405 nm, 488 nm, 561 nm, 640 nm

Detection:

4x GaAsP, galvo + resonant scanner; spectral range 400 – 750 nm

Filters:

DAPI, GFP, mCherry/Cy5, CFP/YFP (eyepieces)

Incubator:

Yes

Contact person:

Link:


Microscope:

Olympus FV3000

Location:

Building 13, room 514E

Usage:

Optical sectioning (CLSM), multi-tiling

Lasers:

405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm

Detection:

4x GaAsP, galvo + resonant scanner; spectral range 400 – 800 nm

Filters:

DAPI, GFP, mCherry/Cy5, CFP/YFP (eyepieces)

Incubator:

Yes

Link:


Microscope:

Olympus FV3000 + RappOpto UGA-42

Location:

Building 13, room 512

Usage:

Optical sectioning (CLSM), multi-tiling, FRAP, optogenetics, photo-crosslinking, laser nano-surgery, ablation, uncaging, DNA damaging

Lasers:

405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm, pulsed for ablation 355 nm and 375 nm

Detection:

4x GaAsP, RappOptoelectronics UGA-42 scanner; spectral range 400 – 800 nm

Filters:

DAPI, GFP, RFP (eyepieces)

Incubator:

No

Link:


Microscope:

Zeiss LSM 780 NLO

Location:

Building 13, room 516D

Usage:

Multi-photon, Second harmonics generation (SHG), Optical sectioning (CLSM), photo activation, FRAP, FCS, FRET, uncaging, laser nano-surgery

Lasers:

405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594 nm, 633 nm, pulsed 690-1064 nm

Detection:

2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm

Filters:

DAPI, GFP, Ds-Red (eyepieces)

Incubator:

Yes

Link:


Microscope:

Zeiss LSM 880 AIRY fast

Location:

Building 13, room 516D

Usage:

Airy scan, Optical sectioning (CLSM), multi-tiling, FCS, feedback microscopy

Lasers:

405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm

Detection:

2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm

Filters:

DAPI, GFP, Ds-Red (eyepieces)

Incubator:

Yes

Link:


Microscope:

Zeiss LSM 980 AIRY fast

Location:

Building 13, room 516C

Usage:

Airy scan, Optical sectioning (CLSM), multi-tiling, FCS, feedback microscopy

Lasers:

405 nm, 488 nm, 561 nm, 639 nm

Detection:

2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm, Axiocam 506 mono

Filters:

DAPI, GFP, Cy3, Penta (eyepieces + camera)

Incubator:

Yes

Link:


Microscope:

Zeiss LSM 900

Location:

Building 13, room 515A

Usage:

Optical sectioning (CLSM), multi-tiling, feedback microscopy

Lasers:

405 nm, 488 nm, 561 nm, 640 nm

Detection:

3x GaAsP; spectral range 405 – 750 nm, Axiocam 503 mono

Filters:

DAPI, FITC, TRITC, Cy5, Quad (eyepieces + camera)

Incubator:

Yes

Link:


Widefield


Microscope:

Nikon Ti-2

Location:

Building 13, room 508E

Usage:

Color- and monochromatic imaging, multi-tiling, JOBS module, feedback microscopy

Fluo lamp:

LUMENCOR SPECTRA III

Detection:

Hamamatsu Orca Fusion CMOS mono + DS-Ri2 color

Filters:

GFP, mCherry, Penta (camera)

Incubator:

No

Contact person:

Link:


Microscope:

Nikon Ti-E

Location:

Building 13, room 514A

Usage:

Monochromatic imaging, multi-tiling, JOBS module, feedback microscopy

Fluo lamp:

LUMENCOR SPECTRA X

Detection:

Hamamatsu Orca Flash 4 V2 mono

Filters:

DAPI, GFP, Cy3, mCherry, Cy5 (camera)

Incubator:

Yes

Contact person:

Link:


Microscope:

Nanolive 3D cell Explorer Fluo

Location:

Building 13, room 509B

Usage:

Holotomographic imaging, Label-free imaging

Fluo lamp:

CooLED pE300

Detection:

Camera based

Filters:

DAPI-FITC-TRITC/Cy5 (camera)

Incubator:

Yes

Contact person:

Link:


Microscope:

Zeiss Microbeam (LMD)

Location:

Building 13, room 508D

Usage:

Laser micro-dissection

Fluo lamp:

Pulsed 355 nm laser, HXP 120 C metal halide lamp

Detection:

Axiocam 506 mono

Filters:

DAPI, GFP, RFP (camera)

Incubator:

No

Contact person:

Link:


Spinning Disc


Microscope:

Olympus iXplore SPIN SR

Location:

Building 13, room 515C

Usage:

High speed confocal and super resolution (SoRA), Dual-cam imaging

Laser:

405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm

Detection:

2x ORCA-FLASH4.0 V3 mono

Filters:

DAPI, CFP, FITC, YFP, Cy3, mCherry, Cy5 (camera)

Incubator:

Yes

Link:


Microscope:

Nikon Ti-2 CSU W1 SORA Spinning disk

Location:

Building 13, room 508F

Usage:

High speed confocal and super resolution (SoRA), Dual-cam imaging

Laser:

405 nm, 488 nm, 561 nm, 640 nm

Detection:

2x Hamamatsu Orca FusionBT mono

Filters:

tba

Incubator:

Yes

Link:


High throughput


Microscope:

Molecular Devices IXM

Location:

Building 13, room 508B

Usage:

Imaging multi-well plates and cell cultures

Fluo lamp:

Solid state light source

Detection:

Andor Zyla mono

Filters:

Hoechst, GFP, Cy3, TxRed, Cy5 (camera)

Incubator:

Yes (temperature only)

Link:


Microscope:

Nikon Ti-E Plateloader

Location:

Building 13, room 508B

Usage:

Equipped with plate loading robot for 20 plates, JOBS module

Fluo lamp:

Lumencor Sola SE II 365

Detection:

DS-Qi2 mono

Filters:

DAPI, FITC, Cy3, mCherry, Cy5 (camera)

Incubator:

No

Contact person:

Link:


Microscope:

Olympus ScanR IX83

Location:

Building 13, room 508B

Usage:

High throughput

Fluo lamp:

LED lamp from CoolLED

Detection:

Hamamatsu ORCA-Flash4.0 VS mono

Filters:

DAPI, FITC, mCherry, Cy5 (camera)

Incubator:

Yes

Contact person:

Link:


Microscope:

Zeiss LSM 900

Location:

Building 13, room 515A

Usage:

Optical sectioning (CLSM), multi-tiling, feedback microscopy

Lasers:

405 nm, 488 nm, 561 nm, 640 nm

Detection:

3x GaAsP; spectral range 405 – 750 nm, Axiocam 503 mono

Filters:

DAPI, FITC, TRITC, Cy5, Quad (eyepieces + camera)

Incubator:

Yes

Link:


Light Sheet


Microscope:

Leica SP8 DLS

Location:

Building 13, room 509C

Usage:

Lighsheet (SPIM)

Lasers:

405 nm, 488 nm, 552 nm, 638 nm

Detection:

DLS: Hamamatsu Orca Flash

Filters:

DAPI, FITC, Quad (for lighsheet)

Incubator:

Yes

Link:


Microscope:

Luxendo MuVi SPIM

Location:

Building 13, room 509B

Usage:

Lighsheet (SPIM)

Lasers:

445 nm, 488 nm, 515 nm, 561 nm, 642 nm

Detection:

2x Hamamatsu orca flash 4 mono

Filters:

CFP, FITC/GFP, YFP, mCherry, Cy5 (camera)

Incubator:

Yes

Contact person:

Link:


Super resolution


Microscope:

Abbelight Olympus Safe360 (TIRF, SMLM)

Location:

Building 13, room 509A

Usage:

TIRF, HiLo, SMLM

Lasers:

405 nm, 488 nm, 530 nm, 640 nm

Detection:

2x Hamamatsu Orca Fusion mono

Filters:

Cy3 / Cy5, 560 nm BS (camera)

Incubator:

No

Contact person:

Link:


Microscope:

Leica SP8 X STED 3D

Location:

Building 13, room 516B

Usage:

Stimulated emission depletion, Optical sectioning (CLSM), multi-tiling, FRAP, Navigator

Lasers:

405 nm, 458 nm, 476 nm, 488 nm, 496 nm, 514 nm, WLL (pulsed 470 – 670 nm); STED lasers 592 nm, 660 nm and pulsed 775 nm

Detection:

2x PMT, 2x HyD; spectral range 400 – 800 nm

Filters:

DAPI, FITC, RFP (eyepieces)

Incubator:

Yes

Contact person:

Link:


Multiphoton


Microscope:

Zeiss LSM 780 NLO

Location:

Building 13, room 516D

Usage:

Multi-photon, Second harmonics generation (SHG), Optical sectioning (CLSM), photo activation, FRAP, FCS, FRET, uncaging, laser nano-surgery

Lasers:

405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594 nm, 633 nm, pulsed 690-1064 nm

Detection:

2x PMT, 1x Array GaAsP; spectral range 370 – 800 nm

Filters:

DAPI, GFP, Ds-Red (eyepieces)

Incubator:

Yes

Link:


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