The fundamentals of high-end cell sorting

Overview

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Course overview

Flow cytometry-based cell sorting remains a cornerstone technique across many areas of life science research. As access to high-end sorters increases, researchers are often expected to operate these instruments with minimal institutional support or formal training – particularly in settings lacking dedicated core facilities. Yet, understanding  the principles behind cell sorting is crucial for designing effective experiments, troubleshooting issues, and generating high-quality, publishable data.

This practical course addresses that need by offering a comprehensive programme that blends theoretical foundations with hands-on experience. Delivered by leading experts, it provides in-depth instruction on established best practices while also introducing participants to the latest advancements in the field, including spectral and image-enabled cell sorting. Now a well-established programme, the course is continuously refined to reflect technological advancements and the evolving needs of the research community. Each edition incorporates participant feedback and introduces emerging topics – such as spectral and image-enabled sorting, data reproducibility, and downstream applications. For example, image-enabled cell sorting, to which our team has directly contributed (Schraivogel et al. 2022), is now a key part of the programme. This dynamic and responsive approach ensures that the course remains current, relevant, and impactful for researchers working with advanced cytometry technologies.   

What past participants say about the course

“This course provides an exceptional hands-on experience with the latest techniques in flow cytometry. The expert-led sessions and collaborative environment make it an invaluable resource for expanding practical skills and fostering connections in the field of cell and particle sorting.” – Indrė Dalgėdienė, Vilnius University Life Sciences Center, Lithuania

“Everybody who does cell sorting should take part in this course. I learned so much that helped me improve my experiments and guided me toward solving my problems. If I succeed in my experiments, it is in large part due to this course.” – Veronika Pfaffenstaller, Biomedical Center Munich at the LMU, Germany

“I had high expectations for this course on the Fundamentals of high-end cell sorting. But my expectations were widely surpassed! I have learnt so much during this week, including things that I thought I was almost an expert in. I can’t wait to go back to the lab and apply everything I learnt! I do recommend this course for anyone planning to do cell sorting!” – Luís Crisóstomo, University of Turku, Finland

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