Currently, users can investigate a wide range of proteins under physiological conditions and undertake functional studies investigating the effects of differing temperatures (2-50 degrees ºC), pH (4-10), salt concentration and/or the addition of ligands in samples as small as 5 µl (25-30 µl are recommended). The bioSAXS beamline is dedicated to measuring biological macromolecules in solution with the aim of providing a simple to use facility with rapid access using the ESRF MX BAG or rolling application procedures.
A high level of automation is implemented to aid users with data collection, rapid preliminary analysis and online quality control using an EMBL-ESRF implementation of the data analysis tools developed by EMBL Hamburg and displayed in the Extended ISPyB for bioSAXS (Exi-ISPyBB) experimental database. This provides users with valuable feedback regarding their experiment to maximise throughput, dataset completeness and the possible need for additional measurements.
As part of the Partnership for Structural Biology (PSB) Small Angle Scattering (SAS) platform there is a unified application procedure within the ILL application procedure to enable joint SAXS/SANS experiments at the ILL and the ESRF facilities in one visit to the EPN site.
The BioSAXS beamline is run as a collaboration between the EMBL Grenoble Synchrotron Crystallography Team and the ESRF Structural Biology Group. See the beamline webpage for more information on the technical details and the ESRF website for application details.
Oskarsson, M.C., Beteva, A., De Sanctis, D., Leonard, G., Pernod, P., Tully, M.D., Florial, J.B., McCarthy, A.A. From MxCuBE3 to BSXCuBE3 a web based application for BioSAXS experiment control. 17th Int. Conf. on Accelerator and Large Experimental Physics Control Systems (ICALEPCS'19), New York, NY, USA, (2019), WEPHA115
Rodríguez-Ruiz, I., Radajewski, D., Charton, S., Phamvan, N., Brennich, M., Pernot, P., Bonneté, F., Teychené, S. Innovative High-Throughput SAXS Methodologies Based on Photonic Lab-on-a-Chip Sensors: Application to Macromolecular Studies. Sensors (Basel) 17 (2017)
Brooks-Bartlett, J.C., Batters, R.A., Bury, C.S., Lowe, E.D., Ginn, H.M., Round, A., Garman, E.F. Development of tools to automate quantitative analysis of radiation damage in SAXS experiments. J Synchrotron Radiat 24, 63-72 (2017).
Costa, L., Andriatis, A., Brennich, M., Teulon, J.M., Chen, S.W.W, Pellequer, J.L., Round, A. Combined small angle X-ray solution scattering with atomic force microscopy for characterizing radiation damage on biological macromolecules. BMC STRUCT BIOL 16, 1-13 (2016)
Hutin, S., Brennich, M., Maillot, B., Round, A. Online ion-exchange chromatography for small-angle X-ray scattering. Acta Crystallogr D Struct Biol 72,1090-1099 (2016).
De Maria Antolinos, A., Pernot, P., Brennich, M. E., Kieffer, J., Bowler, M. W., Delageniere, S., Ohlsson, S., Malbet Monaco, S., Ashton, A., Franke, D., Svergun, D., McSweeney, S., Gordon, E., Round, A. ISPyB for BioSAXS, the gateway to user autonomy in solution scattering experiments. Acta Crystallogr. D Biol. Crystallogr. 71, 76-85 (2015).
Round, A., Felisaz, F., Fodinger, L., Gobbo, A., Huet, J., Villard, C., Blanchet, C. E., Pernot, P. McSweeney, S., Roessle, M., Svergun, D. I., Cipriani, F. BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments Acta Crystallogr. D Biol. Crystallogr. 71, 67-75 (2015).
Pernot, P., Round, A., Barrett, R., De Maria Antolinos, A., Gobbo, A., Gordon, E., Huet, J., Kieffer, J., Lentini, M., Mattenet, M., Morawe, C., Mueller-Dieckmann, C., Ohlsson, S., Schmid, W., Surr, J., Theveneau, P., Zerrad, L., McSweeney, S. Upgraded ESRF BM29 beamline for SAXS on macromolecules in solution. J. Synchrotron Rad. 20, 660-664 (2013).