Eukaryotic Expression Facility

Two biosafety level 1 labs for protein expression in insect cells via notably the MultiBac-EMBL system:

Introduction to baculovirus/insect cell expression
Introduction to MultiBac-EMBL system for multiprotein complex production
Providing transfer vectors and protocols for multigene expression cassette generation
Providing E. coli cells for composite bacmid generation
Providing insect cells for virus generation and amplification
Viral performance and protein production monitoring
Small, medium, and large scale expression of proteins and protein complexes

Two biosafety level 2 labs for the growth of mammalian cells, either adherent or in suspension:

Introduction to mammalian cell culture
Introduction to recombinant protein expression in mammalian cell
Design of constructs and generation of stable cell line (e.g. CRISPR/Cas9)
Providing reagents and protocols for large cargo delivery using BacMAM

In addition to all standard equipment, the platform also offers access to Light and Confocal Microscopes matching users' needs.

Services at EMBL Grenoble

EMBL Grenoble provides research services including access to its high throughput crystallisation facility (HTX), the fourth-generation synchrotron beamlines for macromolecular crystallography and small-angle scattering at the ESRF, and the PSB-run Titan Krios cryo-electron microscope CM01 at the ESRF.

Eukaryotic Expression Facility beta

EMBL Grenoble

Two biosafety level 1 labs for protein expression in insect cells via notably the MultiBac-EMBL system:

Two biosafety level 2 labs for the growth of mammalian cells, either adherent or in suspension:

Team