Two biosafety level 1 labs for protein expression in insect cells via notably the MultiBac-EMBL system:
- Introduction to baculovirus/insect cell expression
- Introduction to MultiBac-EMBL system for multiprotein complex production
- Providing transfer vectors and protocols for multigene expression cassette generation
- Providing E. coli cells for composite bacmid generation
- Providing insect cells for virus generation and amplification
- Viral performance and protein production monitoring
- Small, medium, and large scale expression of proteins and protein complexes
Two biosafety level 2 labs for the growth of mammalian cells, either adherent or in suspension:
- Introduction to mammalian cell culture
- Introduction to recombinant protein expression in mammalian cell
- Design of constructs and generation of stable cell line (e.g. CRISPR/Cas9)
- Providing reagents and protocols for large cargo delivery using BacMAM
In addition to all standard equipment, the platform also offers access to Light and Confocal Microscopes matching users' needs.