Synergistic activity of rifampicin and polymyxin B against intracellular Gram-negative ESKAPE pathogens involves bacterial membrane alterations and enhanced oxidative damages.

Varik V, Wang G, Kritikos G, Banzhaf M, Drouot E, Koumoutsi A, Van Bambeke F

Antimicrobial agents and chemotherapy, 2026

doi:10.1128/aac.01319-25.

Uncovering nitroxoline activity spectrum, mode of action and resistance across Gram-negative bacteria.

Cacace E, Tietgen M, Steinhauer M, Mateus A, Schultze TG, Eckermann M, Galardini M, Varik V, Koumoutsi A, Parzeller JJ, Corona F, Orakov A, Knopp M, Brauer-Nikonow A, Bork P, Romao CV, Zimmermann M, Cloetens P, Savitski MM, Typas A, Göttig S

Nature communications, 2025

doi:10.1038/s41467-025-58730-5.

Randomly barcoded transposon mutant libraries for gut commensals I: Strategies for efficient library construction.

Tripathi S, Voogdt CGP, Bassler SO, Anderson M, Huang PH, Sakenova N, Capraz T, Jain S, Koumoutsi A, Bravo AM, Trotter V, Zimmerman M, Sonnenburg JL, Buie C, Typas A, Deutschbauer AM, Shiver AL, Huang KC

Cell reports, 2024

doi:10.1016/j.celrep.2023.113517.

Randomly barcoded transposon mutant libraries for gut commensals II: Applying libraries for functional genetics.

Voogdt CGP, Tripathi S, Bassler SO, McKeithen-Mead SA, Guiberson ER, Koumoutsi A, Bravo AM, Buie C, Zimmermann M, Sonnenburg JL, Typas A, Deutschbauer AM, Shiver AL, Huang KC

Cell reports, 2024

doi:10.1016/j.celrep.2023.113519.

Phenotype inference in an Escherichia coli strain panel.

Galardini M, Koumoutsi A, Herrera-Dominguez L, Cordero Varela JA, Telzerow A, Wagih O, Wartel M, Clermont O, Denamur E, Typas A, Beltrao P

eLife, 2017

doi:10.7554/eLife.31035.

A tool named Iris for versatile high-throughput phenotyping in microorganisms.

Kritikos G, Banzhaf M, Herrera-Dominguez L, Koumoutsi A, Wartel M, Zietek M, Typas A.

Nature Microbiology, 2017

doi:10.1038/nmicrobiol.2017.14.

Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division.

Gray AN, Egan AJ, Van't Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar MA, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W

eLife, 2015

doi:10.7554/elife.07118.

Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B.

Egan AJ, Jean NL, Koumoutsi A, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A, Vollmer W, Simorre JP

Proceedings of the National Academy of Sciences of the United States of America, 2014

doi:10.1073/pnas.1400376111.