Research Team beta

Chris Bowler

Environmental and Evolutionary Genomics Section

Institut de Biologie de l’Ecole Normale Supérieure (IBENS), France

Diatoms are unicellular photosynthetic eukaryotes with glass exoskeletons believed to be derived from an ancient secondary endosymbiosis between a heterotroph with green and red algae. They are one of the major phytoplankton groups in the ocean, contributing around 40% oceanic primary production and playing a key role in the biological carbon pump through which atmospheric CO2 is sequestered to the ocean interior.

They are prevalent in upwelling and polar regions, and often form huge blooms which proliferate rapidly in response to episodic nutrient injection events, and then disappear as a result of population-level programmed cell death phenomena. Two complete genome sequences have been reported, allowing diatom-derived environmental sequences to be identified, and their well characterized morphologies provide valuable information for taxon identification.

Knowledge of diatom molecular responses to environmental stimuli is relatively advanced. Several questions of high scientific interest can be addressed using Tara Oceans data. Most broadly, we aim to generate a global view of diatom distributions and biodiversity using V9 rDNA tag data, and to relate it to cell morphology and to different physico-chemical parameters such as light, temperature, nutrients, alkalinity and mixing at different scales, as well as with respect to population density and overall community composition.

Furthermore, functional studies can be performed using metagenomics and metatranscriptomics data from defined oceanic settings, and by sequencing genomes and transcriptomes from individual diatom cells collected from the environment, and by elucidating defined aspects of diatom cell physiology by high throughput confocal imaging using a range of selected fluorescent dyes that label specific subcellular components.

Relevant publications

  1. Allen AE, Dupont CL, Obornik M, Horak A, Nunes-Nesi A, McCrow JP, Zheng H, Johnson DA, Hu H, Fernie AR, Bowler C. (2011) Evolution and metabolic significance of the urea cycle in photosynthetic diatoms. Nature 473: 203-207.
  2. Moustafa A, Beszteri B, Maier UG, Bowler C, Valentin, K., and Bhattacharya, D. (2009) Genomic footprints of a cryptic plastid endosymbiosis in diatoms. Science 324:1724-1726.
  3. Bowler C, Karl DM, Colwell RR. (2009) Microbial oceanography in a sea of opportunity. Nature 459: 180-184.
  4. Bowler C, et al. (2008) The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature 456: 239-244.
  5. Armbrust EV, Berges JB, Bowler C et al. (2004) The genome of the diatom Thalassiosira pseudonana: Ecology, evolution, and metabolism. Science 304: 79-86.
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The scientific activities of the Tara Oceans expedition, led by EMBL senior scientist Eric Karsenti, present an unprecedented effort that resulted in 35,000 samples containing millions of small organism collected in more than 210 ocean stations, chosen for their climatic significance or biodiversity.