Beamline ID 30B

The ESRF and EMBL Grenoble have long worked together for the provision of advanced macromolecular crystallography (MX) beamlines. Towards this goal, ESRF and EMBL Grenoble constructed and commissioned a tunable, high-intensity and variable focus MX beamline on ID30B at the ESRF. The EMBL Grenoble beamline scientist for ID30B is Andrew McCarthy, team leader of the Synchrotron Crystallography Team, who is co-responsible for the beamline and has been involved in the project since its inception.

ID30B is equipped with a MD2S diffractometer, the first of its kind, enabling data collection in both standard cryogenic and in situ crystallisation plate formats. The MD2S and PILATUS3 6M detector allow sophisticated synchronous high-speed data collections such as multi-orientation data collection, raster scanning and synchrotron serial crystallography techniques. To facilitate the increased throughput, the beamline has been equipped with a new-generation EMBL-ESRF flexible sample changer (FlexHCD) based on 6-axis robotics that is capable of transferring pins mounted in both Grenoble and Unipuck-type formats.

ID30B returned to user operation in August 2020 following the ESRF Extremely Brilliant Source (EBS) upgrade with exceptional X-ray characteristics to ensure it will remain at the forefront of tunable crystallography beamlines.

ID30B is run as a collaboration between the EMBL-Grenoble Synchrotron Crystallography Team and the ESRF Structural Biology Group. See the ID30B webpage for more information on the technical details and the ESRF website for application details.

lab equipment
View of the ID30B experimental hutch

Reference publications

Gavira, J.A., Rodriguez-Ruiz, I., Martinez-Rodriguez, S., Basu, S., Teychené, S., McCarthy, A.A., Mueller-Dieckman, C. Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips. Acta Crystallogr D Struct Biol 76:751-758 (2020).

Felisaz, F., Sinoir, J., Papp, G., Pica, A., Bowler, M.W., Murphy, P., Hoffmann, G., Zander, U., Lopez-Marrero, M., Janocha, R., Giraud, T., Svensson, O., Popov, S., Leonard, G., Mueller-Dieckmann, C., Márquez, J.A., McCarthy, A., Cipriani, F. CrystalDirect-To-Beam: Opening the shortest path from crystal to data. AIP Conference Proceedings 2054 (2019).

McCarthy, A. A., Barrett, R., Beteva, A., Caserotto, H., Dobias, F., Felisaz, F., Giraud, T., Guijarro, M., Janocha, R., Khadrouche, A., Lentini, M., Leonard, G. A., Lopez Marrero, M., Malbet-Monaco, S., McSweeney, S., Nurizzo, D., Papp, G., Rossi, C., Sinoir, J., Sorez, C., Surr, J., Svensson, O., Zander, U., Cipriani, F., Theveneau, P., Mueller-Dieckmann, C. ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF. J. Synchrotron Radiat. 25,1249-1260 (2018).

Mueller-Dieckmann, C., Bowler, M. W., Carpentier, P., Flot, D., McCarthy, A. A., Nanao, M. H., Nurizzo, D., Pernot, P., Popov, A., Round, A., Royant, A., de Sanctis, D., von Stetten, D., Leonard, G. A. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility, Eur. Phys. J. Plus130, 70 (2015).

EMBL Grenoble collaborates with the Structural Biology group at the ESRF (European Synchrotron Radiation Facility) in the design, construction, operation and development of synchrotron beamlines for macromolecular crystallography (MX) and small angle X-ray scattering (SAXS).