In the context of the Partnership for Structural Biology (PSB), combinatorial approaches, screening and robotics are applied to develop novel methods to solve challenging problems of protein expression for structural biology.
Difficult biological projects require advanced new tools. We will continue to develop expression methods to handle protein complexes, targets that require eukaryotic expression for correct folding, and possibly aspects of membrane proteins.
Each project uses “real” targets of interest and the aim is use method advancements to yield previously unobtainable biological knowledge. For example, we are testing permutations of influenza-influenza and influenza-host proteins with the aim of defining expressible, crystallisable protein complexes that should provide insights into virus host cell interactions.