{"id":7584,"date":"2016-08-30T12:23:05","date_gmt":"2016-08-30T10:23:05","guid":{"rendered":"http:\/\/news.embl.de\/?p=7584"},"modified":"2024-03-25T10:24:29","modified_gmt":"2024-03-25T09:24:29","slug":"1608-jochen-wittbrodt","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/alumni\/1608-jochen-wittbrodt\/","title":{"rendered":"Swimming against the tide"},"content":{"rendered":"\n<p>Wittbrodt clashed with the impossible in his very first position as group leader, at the Max Planck Institute for Biophysical Chemistry in G\u00f6ttingen. He decided to run genetic screens on Medaka fish. In a genetic screen, scientists introduce mutations into an organism\u2019s genes, and systematically look at the effects of those mutations on aspects like physical traits, physiology and behaviour. This is a laborious process \u2013 and was even more so back in the early 1990s, when gene sequencing was in its infancy \u2013 so the labs carrying out such studies tended to rely on a large workforce, and focus on established model organisms such as zebrafish. So Wittbrodt\u2019s proposal to tackle a species for which no-one had yet developed specific tools, with a lab of 6 people, was met with scepticism. \u201cBut we just did it, and it worked,\u201d he chuckles.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Birth of SPIM<\/h2>\n\n\n\n<p>A decade later, he had set up a lab at EMBL, and a presentation at a conference sparked another daring idea. \u201cPete Curry was showing an image of a whole fish in 3D imaged by a new method called OPT,\u201d he recalls. \u201cWhen I came back to Heidelberg, I met Ernst Stelzer in the EMBL cafeteria, and I said: \u2018We need that, but we need it in live fish!\u2019\u201d Wittbrodt eventually convinced Stelzer to put a student on the job, and single-plane illumination microscopy (SPIM) was born. The basic idea of SPIM sounded like science fiction, says Wittbrodt: having a living organism under the microscope, shining a sheet of light on one layer of cells at a time, and putting that information back together to get a picture of the whole animal. The technique enabled Wittbrodt\u2019s lab to see a fish embryo\u2019s beating heart, and to trace its optic nerve from eye to brain. But he saw an opportunity to probe deeper \u2013 another \u2018wild idea\u2019: tracking each cell in an embryo, as they divide and move throughout the first day of its life. \u201cTextbooks said you couldn\u2019t do this in a vertebrate,\u201d he says, \u201cuntil Philipp Keller, who was then a PhD student in the Stelzer group, developed Digital Scanned Laser Light Sheet Microscopy (DSLM), and we saw how long we could image embryos for \u2013 and then we went for it!\u201d The result, which they dubbed the Digital Embryo, earned them recognition from <em>Science<\/em> as one of the breakthroughs of the year for 2008.<\/p>\n\n\n\n<blockquote class=\"vf-blockquote\">\n<p>If I have an aim, I\u2019ll find a solution<\/p>\n<\/blockquote>\n\n\n\n<p>Since leaving EMBL, Wittbrodt has continued to work on further developments to the technology, collaborating with <a href=\"http:\/\/www.embl.de\/research\/units\/cbb\/hufnagel\/index.html\">Lars Hufnagel<\/a> in particular.&nbsp; He has another wild idea up his sleeve. \u201cYou\u2019d have the fish swimming freely, and passing through a laser scanner, being imaged as it goes.\u201d Such a naturalistic setting would allow scientists to probe questions that are currently out of bounds, but so far, this particular idea has proven too \u2018sci-fi\u2019 for even Wittbrodt to get anyone to bite on.<\/p>\n\n\n\n<p>How does he keep up his own drive in such situations, and keep swimming against the current? \u201cSome people see the problems along the way, but forget what we\u2019re aiming at. I think I can worry about a problem, but if I have an aim, I\u2019ll find a solution, a way around it,\u201d he replies.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Radical vision<\/h2>\n\n\n\n<p>Fostering and developing this type of unconventional approach is something that Wittbrodt has built into the workings of his lab at the University of Heidelberg. \u201cI always give some \u2018crazy vision\u2019 talk once a year, at one of our lab meetings,\u201d he says. One of the ideas he presented at this year\u2019s talk was a new way to probe what conditions stem cells need in order to grow. His vision: to print an unconventional 3D scanner. \u201cThis scanner would not be a scanner in the sense that it scans your material; instead, your material \u2013 in our case stem cells \u2013 would scan the scanner,\u201d he explains. The idea would be to use 3D printing technology to create a habitat for cells; hundreds of thousands of alcoves with different properties \u2013 some spongier, some harder, some with one type of food, others with another\u2026 Wittbrodt and colleagues would place their stem cells on this habitat, and let the cells choose their niche. \u201cAnd we could just read out the properties and know what the stem cell needs,\u201d he says, noting that he already has engineers, chemists and physicists, as well as specialists in 3D printing, on board.<\/p>\n\n\n\n<p>Biologists have to be conversant in other fields in order to dream up \u2013 and implement \u2013 these approaches, he emphasises. \u201cSpecialisation gives you more details, and will keep some people happy,\u201d he concedes. \u201cBut there are others who like to maybe bridge the boundaries and live in the interface, and try to get new ideas at the edges \u2013 this is much more the way I see myself. I wouldn\u2019t say that I\u2019m a zoologist, or a geneticist, or a cell biologist; I need a little bit of everything.\u201d<\/p>\n\n\n\n<p>To anyone who feels similarly, and has an \u2018impossible\u2019 idea that just might work, he says: \u201cfollow your heart; trust your gut!\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#8220;If I have an aim, I\u2019ll find a solution&#8221; \u2013 meet EMBL alumnus Jochen Wittbrodt<\/p>\n","protected":false},"author":8,"featured_media":7586,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[5,17593],"tags":[80,43,49,694],"embl_taxonomy":[],"class_list":["post-7584","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-alumni","category-people-perspectives","tag-alumni","tag-heidelberg","tag-interdisciplinary","tag-spim"],"acf":{"article_intro":"<p>Give EMBL alumnus Jochen Wittbrodt ten minutes of your time, and he\u2019ll convince you just how much biologists have to gain from embracing the impossible, pursuing outlandish ideas, and living on the edge<\/p>\n","related_links":[{"link_description":"","link_url":""}],"article_sources":false,"vf_locked":false,"featured":false,"color":"#007B53","show_featured_image":false,"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"source_article":false,"in_this_article":false,"press_contact":"None","article_translations":false,"languages":""},"embl_taxonomy_terms":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Swimming against the tide: insights from alumnus Jochen Wittbrodt<\/title>\n<meta name=\"description\" content=\"Give EMBL alumnus Jochen Wittbrodt ten minutes of your time, and he\u2019ll convince you just how much biologists have to gain from embracing the impossible\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/alumni\/1608-jochen-wittbrodt\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Swimming against the tide: insights from alumnus Jochen Wittbrodt\" \/>\n<meta property=\"og:description\" content=\"Give EMBL alumnus Jochen Wittbrodt ten minutes of your time, and he\u2019ll convince you just how much biologists have to gain from embracing the impossible\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/alumni\/1608-jochen-wittbrodt\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2016-08-30T10:23:05+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-03-25T09:24:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2016\/08\/1608-swimming-against.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"620\" \/>\n\t<meta property=\"og:image:height\" content=\"425\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Sonia Furtado Neves\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@Aur_ora\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Sonia Furtado Neves\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/alumni\/1608-jochen-wittbrodt\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/alumni\/1608-jochen-wittbrodt\/\"},\"author\":{\"name\":\"Sonia Furtado Neves\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/d926199a955624b44dda296f396c5e68\"},\"headline\":\"Swimming against the tide\",\"datePublished\":\"2016-08-30T10:23:05+00:00\",\"dateModified\":\"2024-03-25T09:24:29+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/alumni\/1608-jochen-wittbrodt\/\"},\"wordCount\":862,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/alumni\/1608-jochen-wittbrodt\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2016\/08\/1608-swimming-against.jpg\",\"keywords\":[\"alumni\",\"heidelberg\",\"interdisciplinary\",\"spim\"],\"articleSection\":[\"Alumni\",\"People &amp; 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