{"id":73399,"date":"2025-02-20T11:00:00","date_gmt":"2025-02-20T10:00:00","guid":{"rendered":"https:\/\/www.embl.org\/news\/?p=73399"},"modified":"2025-09-18T16:44:19","modified_gmt":"2025-09-18T14:44:19","slug":"pushing-the-limits-of-custom-made-microscopy","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/science-technology\/pushing-the-limits-of-custom-made-microscopy\/","title":{"rendered":"Pushing the limits of \u2018custom-made\u2019 microscopy"},"content":{"rendered":"\n<article class=\"vf-card vf-card--brand vf-card--bordered vf-u-margin__bottom--800\" default>\n  <div class=\"vf-card__content | vf-stack vf-stack--400\">\n      <h3 class=\"vf-card__heading\">\n      Summary    <\/h3>\n                <p class=\"vf-card__text\"><ul>\r\n \t<li><span style=\"font-weight: 400;\">Previously<\/span><b>, <\/b><span style=\"font-weight: 400;\">EMBL scientists developed a new microscope based on Brillouin scattering \u2013 a phenomenon where light interacts with naturally occurring thermal vibrations within materials, from which their mechanical properties can be deduced. This method has since been used for non-invasive and live-imaging applications in biology, and that advance was selected as one of <\/span><a class=\"vf-card_link\" href=\"https:\/\/www.theguardian.com\/science\/2022\/dec\/18\/the-10-biggest-science-stories-of-2022-chosen-by-scientists\"><i><span style=\"font-weight: 400;\">The Guardian<\/span><\/i><span style=\"font-weight: 400;\">\u2019s 10 biggest science stories of 2022<\/span><\/a><span style=\"font-weight: 400;\">.<\/span><\/li>\r\n \t<li><span style=\"font-weight: 400;\">In a new paper, the same scientists have now succeeded in substantially further advancing Brillouin microscopy, making it approximately a thousand times faster and more efficient.\u00a0<\/span><\/li>\r\n \t<li><span style=\"font-weight: 400;\">Simultaneously, the new microscopy method expands the swath of material it can view \u2013 from a 100-pixel line to a ~10,000-pixel full plane, capturing\u00a0 3D images quickly enough for live-organism observation. <\/span><\/li>\r\n<\/ul><\/p>\n      <\/div>\n<\/article>\n\n\n\n\n<p><strong><em>A new paper updates an EMBL technology advance even further.&nbsp; More details about the original technology can be found in our initial reporting <\/em><\/strong><a href=\"https:\/\/www.embl.org\/news\/science\/shining-light-on-the-mechanics-of-embryo-development\/\"><strong><em>here<\/em><\/strong><\/a><strong><em>.&nbsp;<\/em><\/strong><\/p>\n\n\n\n<p>EMBL tech developers have made an important leap forward with a novel methodology that adds an important microscopy capability to life scientists\u2019 toolbox. The advance represents a 1,000-fold improvement in speed and throughput in Brillouin microscopy and provides a way to view light-sensitive organisms more efficiently.<\/p>\n\n\n\n<p>\u201cWe were on a quest to speed up image acquisition,\u201d said Carlo Bevilacqua, lead author on a paper published about this technological development in <em>Nature Photonics<\/em> and an optical engineer in EMBL\u2019s Prevedel Team. \u201cOver the years, we have progressed from being able to see just a pixel at a time to a line of 100 pixels, to now a full plane that offers a view of approximately 10,000 pixels.\u201d<\/p>\n\n\n\n<p>The technology is based on a phenomenon first predicted in 1922 by French physicist L\u00e9on Brillouin. He showed that when light is shone on a material, it interacts with naturally occurring thermal vibrations within, exchanging energy and thereby slightly shifting the frequency (or colour) of the light. Measuring the spectrum (colours) of the scattered light reveals information about a material\u2019s physical characteristics.&nbsp;<\/p>\n\n\n\n<p>Using Brillouin scattering for microscopy purposes came much later \u2013 in the early 2000s \u2013 when other technological advancements enabled scientists to measure tiny frequency shifts with high precision and sufficient throughput. This allowed them to compute mechanical properties of living biological samples. However, at that point, scientists were only able to view one pixel at a time. The process was therefore quite time-consuming, and it severely limited how the microscopy method could be used in biology. In 2022, Bevilacqua and others in the Prevedel group were able to first expand the field of view to a line, and now with this latest development, to a full 2D field of view, which also helps speed up 3D imaging.&nbsp;<\/p>\n\n\n\n<p>\u201cJust as the development of light-sheet microscopy here at EMBL marked a revolution in light microscopy because it allowed for faster, high-resolution, and minimally phototoxic imaging of biological samples, so too does this advance in the area of mechanical or Brillouin imaging,\u201d said Robert Prevedel, Group Leader and senior author on the paper. \u201cWe hope this new technology \u2013 with minimal light intensity \u2013 opens one more \u2018window\u2019 for life scientists\u2019 exploration.\u201d<\/p>\n\n\n\n<hr class=\"vf-divider\"\/>\n\n\n\n<h1 class=\"wp-block-heading\" id=\"italian\"><strong>Oltre i limiti della microscopia \u201csu misura\u201d<\/strong><\/h1>\n\n\n\n<article class=\"vf-card vf-card--brand vf-card--bordered vf-u-margin__bottom--800\" default>\n  <div class=\"vf-card__content | vf-stack vf-stack--400\">\n      <h3 class=\"vf-card__heading\">\n      Sintesi    <\/h3>\n                <p class=\"vf-card__text\"><ul>\r\n \t<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">In precedenza, un gruppo di scienziati dell&#8217;EMBL (tra cui il ricercatore italiano Carlo Bevilacqua) aveva sviluppato un nuovo microscopio basato sulla diffusione Brillouin, un fenomeno per cui la luce interagisce con le vibrazioni termiche naturali all&#8217;interno dei materiali e da cui si possono dedurre le loro propriet\u00e0 meccaniche. L\u2019applicazione di questo metodo al live-imaging in biologia in maniera non invasiva \u00e8 stata menzionata tra le <\/span><a class=\"vf-card_link\" href=\"https:\/\/www.theguardian.com\/science\/2022\/dec\/18\/the-10-biggest-science-stories-of-2022-chosen-by-scientists\"><span style=\"font-weight: 400;\">10 storie scientifiche pi\u00f9 importanti del 2022 dal The Guardian<\/span><\/a><span style=\"font-weight: 400;\">.<\/span><\/li>\r\n \t<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">In un nuovo lavoro, gli stessi scienziati sono riusciti a migliorare ulteriormente la microscopia Brillouin, rendendola circa mille volte pi\u00f9 veloce ed efficiente.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">La nuova tecnica di microscopia ha ampliato la superficie di materiale che pu\u00f2 essere visualizzata, passando da una linea di 100 pixel a un piano completo di circa 10.000 pixel, il che consente di acquisire immagini tridimensionali abbastanza rapidamente per l&#8217;osservazione di organismi vivi.<\/span><\/li>\r\n<\/ul><\/p>\n      <\/div>\n<\/article>\n\n\n\n\n<p><strong><em>Un nuovo studio aggiorna ulteriormente un progresso tecnologico dell&#8217;EMBL.\u00a0 Maggiori dettagli sulla tecnologia originale sono forniti nell\u2019articolo precedente disponibile <\/em><\/strong><a href=\"https:\/\/www.embl.org\/news\/science\/shining-light-on-the-mechanics-of-embryo-development\/#italian\"><strong><em>qui<\/em><\/strong><\/a><strong><em>.<\/em><\/strong><\/p>\n\n\n\n<p>Gli sviluppatori tecnologici dell&#8217;EMBL hanno compiuto un importante passo avanti con una nuova metodologia che aumenta notevolmente la sensibilit\u00e0 delle tecniche di microscopia applicate allo studio delle scienze della vita. Il progresso rappresenta un miglioramento di 1.000 volte nella velocit\u00e0 e nella produttivit\u00e0 della microscopia Brillouin e consente di visualizzare gli organismi sensibili alla luce in modo pi\u00f9 efficiente.<\/p>\n\n\n\n<p>\u201cEravamo alla ricerca di un modo per aumentare la velocit\u00e0 di acquisizione delle immagini\u201d, ha detto Carlo Bevilacqua, autore principale dell\u2019articolo pubblicato su Nature Photonics e ingegnere ottico del team Prevedel dell&#8217;EMBL di Heidelberg. \u201cNel corso degli anni, siamo passati dalla possibilit\u00e0 di vedere solo un pixel alla volta a una linea di 100 pixel, fino ad arrivare a un piano completo che offre una visione di circa 10.000 pixel\u201d.<\/p>\n\n\n\n<p>La tecnologia si basa su un fenomeno previsto per la prima volta nel 1922 dal fisico francese L\u00e9on Brillouin. Egli dimostr\u00f2 che quando la luce viene irradiata su un materiale, interagisce con le vibrazioni termiche naturalmente presenti al suo interno, scambiando energia e quindi spostando leggermente la frequenza (o il colore) della luce. La misurazione dello spettro (dei colori) della luce diffusa rivela informazioni sulle caratteristiche fisiche di un materiale.&nbsp;<\/p>\n\n\n\n<p>L&#8217;uso della diffusione Brillouin per la microscopia \u00e8 arrivato molto pi\u00f9 tardi, all&#8217;inizio degli anni 2000, quando altri progressi tecnologici hanno permesso agli scienziati di misurare minuscoli spostamenti di frequenza con elevata precisione, misurando cos\u00ec le propriet\u00e0 meccaniche dei campioni biologici viventi. Tuttavia, a quel punto gli scienziati erano in grado di visualizzare solo un pixel alla volta. Il processo richiedeva quindi molto tempo e limitava fortemente l&#8217;utilizzo della microscopia in biologia. Nel 2022, Bevilacqua e altri membri del gruppo Prevedel sono stati in grado di espandere il campo visivo prima a una linea e ora, con questo ultimo sviluppo, a un campo visivo completo in 2-D, che contribuisce a velocizzare anche l&#8217;imaging in 3-D.&nbsp;<\/p>\n\n\n\n<p>\u201cCos\u00ec come lo sviluppo della microscopia a foglietto di luce qui all&#8217;EMBL ha segnato una rivoluzione nella microscopia ottica &#8211; consentendo di ottenere immagini pi\u00f9 rapide, ad alta risoluzione e minimamente fototossiche dei campioni biologici &#8211; anche questo progresso segna una rivoluzione nell&#8217;area dell&#8217;imaging meccanico o Brillouin\u201d, ha dichiarato Robert Prevedel, capogruppo e autore senior dell&#8217;articolo. \u201cSperiamo che questa nuova tecnologia &#8211; con un&#8217;intensit\u00e0 luminosa minima &#8211; apra un&#8217;ulteriore &#8216;finestra&#8217; per l&#8217;esplorazione delle scienze della vita\u201d.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Another EMBL-engineered advance to Brillouin microscopy has significantly widened the aperture to provide quick 3D imaging in real time of light-sensitive samples.<\/p>\n","protected":false},"author":100,"featured_media":73539,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[17591],"tags":[3718,43,13976,79,3720,464,18731],"embl_taxonomy":[9796,9788,19357,19093],"class_list":["post-73399","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-technology","tag-engineering","tag-heidelberg","tag-light-microscopy","tag-microscopy","tag-physics","tag-prevedel","tag-technology-development","embl_taxonomy-embl-heidelberg","embl_taxonomy-embl-rome","embl_taxonomy-prevedel-group","embl_taxonomy-robert-prevedel"],"acf":{"vfwp-news_embl_taxonomy":[19357,19093,9796,9788],"featured":true,"show_featured_image":false,"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"article_intro":"<p>Another EMBL-engineered advance to Brillouin microscopy significantly widens the aperture and provides for quick, more efficient 3-D imaging of light-sensitive samples<\/p>\n","related_links":[{"link_description":"Shining light on the mechanics of embryo development","link_url":"https:\/\/www.embl.org\/news\/science\/shining-light-on-the-mechanics-of-embryo-development\/"},{"link_description":"New microscopy technique makes deep in vivo brain imaging possible","link_url":"https:\/\/www.embl.org\/news\/science\/new-microscopy-technique-makes-deep-in-vivo-brain-imaging-possible\/"},{"link_description":"Spotlight: using light and sound to see into the brain","link_url":"https:\/\/www.embl.org\/news\/picture-of-the-week\/spotlight-using-light-and-sound-to-see-into-the-brain\/"},{"link_description":"Prevedel group","link_url":"https:\/\/www.prevedel.embl.de\/"},{"link_description":"Cell Biology and Biophysics Unit","link_url":"https:\/\/www.embl.org\/research\/units\/cell-biology-biophysics\/"}],"source_article":[{"publication_title":"Full-field Brillouin microscopy based on an imaging Fourier transform spectrometer.","publication_link":{"title":"","url":"https:\/\/www.nature.com\/articles\/s41566-025-01619-y","target":""},"publication_authors":"Bevilacqua C. and Prevedel R.","publication_source":"Nature Photonics","publication_date":"20 February 2025","publication_doi":"10.1038\/s41566-025-01619-y"}],"in_this_article":false,"press_contact":"None","article_translations":[{"translation_language":"Italiano","translation_anchor":"#italian"}],"languages":""},"embl_taxonomy_terms":[{"uuid":"a:3:{i:0;s:36:\"b14d3f13-5670-44fb-8970-e54dfd9c921a\";i:1;s:36:\"89e00fee-87f4-482e-a801-4c3548bb6a58\";i:2;s:36:\"ab46b6d4-71d8-49f8-b2f4-b326d4c8ea4e\";}","parents":[],"name":["EMBL Heidelberg"],"slug":"embl-heidelberg","description":"Where &gt; All EMBL sites &gt; EMBL Heidelberg"},{"uuid":"a:3:{i:0;s:36:\"b14d3f13-5670-44fb-8970-e54dfd9c921a\";i:1;s:36:\"89e00fee-87f4-482e-a801-4c3548bb6a58\";i:2;s:36:\"741d5d3d-f92f-4eb8-9195-55c96454a36b\";}","parents":[],"name":["EMBL Rome"],"slug":"embl-rome","description":"Where &gt; All EMBL sites &gt; EMBL Rome"},{"uuid":"a:3:{i:0;s:36:\"302cfdf7-365b-462a-be65-82c7b783ebf7\";i:1;s:36:\"64999cc4-9a7c-4fea-8339-0e2acc990e08\";i:2;s:36:\"22615b4a-0ac4-4141-8574-199436bb4913\";}","parents":[],"name":["Prevedel Group"],"slug":"prevedel-group","description":"What &gt; Cell biology and biophysics &gt; Prevedel Group"},{"uuid":"a:2:{i:0;s:36:\"4428d1fd-441a-4d6d-a1c5-5dcf5665f213\";i:1;s:36:\"a42830ba-69f4-40ca-8727-80195960e834\";}","parents":[],"name":["Robert Prevedel"],"slug":"robert-prevedel","description":"Who &gt; Robert Prevedel"}],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Pushing the limits of \u2018custom-made\u2019 microscopy | EMBL<\/title>\n<meta name=\"description\" content=\"Another EMBL-engineered advance to Brillouin microscopy has significantly helped enable 3D imaging in real time of light-sensitive samples.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/science-technology\/pushing-the-limits-of-custom-made-microscopy\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Pushing the limits of \u2018custom-made\u2019 microscopy | EMBL\" \/>\n<meta property=\"og:description\" content=\"Another EMBL-engineered advance to Brillouin microscopy has significantly helped enable 3D imaging in real time of light-sensitive samples.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/science-technology\/pushing-the-limits-of-custom-made-microscopy\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2025-02-20T10:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-18T14:44:19+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/02\/20250212_Prevedel_Microscopy3wp.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1000\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Ivy Kupec\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@embl\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Ivy Kupec\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/pushing-the-limits-of-custom-made-microscopy\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/pushing-the-limits-of-custom-made-microscopy\/\"},\"author\":{\"name\":\"Ivy Kupec\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/427f2c9b624bc32ffa67d80414712274\"},\"headline\":\"Pushing the limits of \u2018custom-made\u2019 microscopy\",\"datePublished\":\"2025-02-20T10:00:00+00:00\",\"dateModified\":\"2025-09-18T14:44:19+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/pushing-the-limits-of-custom-made-microscopy\/\"},\"wordCount\":826,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/pushing-the-limits-of-custom-made-microscopy\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/02\/20250212_Prevedel_Microscopy3wp.jpg\",\"keywords\":[\"engineering\",\"heidelberg\",\"light microscopy\",\"microscopy\",\"physics\",\"prevedel\",\"technology development\"],\"articleSection\":[\"Science &amp; 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