{"id":71649,"date":"2024-11-14T10:00:00","date_gmt":"2024-11-14T09:00:00","guid":{"rendered":"https:\/\/www.embl.org\/news\/?p=71649"},"modified":"2024-11-25T17:07:32","modified_gmt":"2024-11-25T16:07:32","slug":"picky-proteins-understanding-yeast-adaptor-protein-selectivity","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/","title":{"rendered":"Picky proteins: understanding yeast adaptor protein selectivity"},"content":{"rendered":"\n<p>The cellular membrane controls the passage of substances in and out of the cell. Some larger cargo, such as membrane proteins, nutrients, and pathogens, enter the cell via a process known as clathrin-mediated endocytosis.<\/p>\n\n\n\n<p>To gain a deeper understanding of this important cellular process, the researchers in the <a href=\"https:\/\/www.embl.org\/groups\/garcia-alai\/\">Garc\u00eda Alai Team<\/a> at EMBL Hamburg and CSSB in collaboration with the <a href=\"https:\/\/www.cssb-hamburg.de\/research\/research_groups\/uetrecht_group\/index_eng.html\">Uetrecht Group<\/a> employed a combination of integrative biophysical and structural approaches together with <em>in vivo<\/em> functional experiments to take a closer look at the interactions between the protein clathrin and various adaptor proteins in budding yeast cells.<\/p>\n\n\n\n<p>During clathrin-mediated endocytosis, adaptor proteins bind to the cell membrane and to the protein clathrin, assisting in engulfing the cargo in a clathrin-coated vesicle.<\/p>\n\n\n\n<p>\u201cThe cargo is essentially sucked into the cell during clathrin-mediated endocytosis. The process is actually quite speedy; everything takes between 60 and 120 seconds \u2013 from the recruitment of the early adaptor proteins to the membrane to the excision of a vesicle,\u201d explained Lucas Defelipe, postdoc in the Garc\u00eda Alai Team and the study\u2019s first author.<\/p>\n\n\n\n<p>The clathrin protein has a three-legged shape and forms cages of different sizes to coat vesicles. At the end of each \u2018clathrin leg\u2019, there is the N-terminal domain (NTD) that interacts with adaptor proteins, linking this scaffold to the cellular membrane. Each NTD has three to four adaptor protein binding sites known as boxes.<\/p>\n\n\n\n<p>To understand the role of these boxes in clathrin-mediated endocytois, the researchers needed to find out whether these NTD boxes have target-specific binding capabilities.<\/p>\n\n\n\n<p>The clathrin heavy chain\u2019s N-terminal domain bound to peptides from specific adaptor proteins was examined using X-ray crystallography at EMBL Hamburg <a href=\"https:\/\/www.embl.org\/groups\/macromolecular-crystallography\/\">beamlines P13 and P14<\/a>. \u201cAll our structures show that each peptide binds to three different binding pockets known as Clathrin box, Arrestin box, and W-box,\u201d explained Mar\u00eda Garc\u00eda-Alai, EMBL Team Leader and the study\u2019s corresponding author. \u201cIn fact, all the peptides were able to adapt to fit into each of these boxes by adopting different conformations.\u201d<\/p>\n\n\n\n<p>Next, the researchers wanted to understand whether the adaptor proteins have any preference for the different boxes. Are they competing with each other to bind to a specific box? To understand the selectivity of adaptors for clathrin, the group conducted several native mass spectrometry (MS) competition experiments at EMBL\u2019s <a href=\"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/\">Sample Preparation and Characterisation Facility<\/a>. \u201cThese experiments revealed that adaptor protein Ent5 is the strongest binder to all NTD boxes, and more importantly, showed that the adaptor proteins Ent1 and Ent2 do in fact have binding preferences,\u201d noted CSSB Group Leader Charlotte Uetrecht, whose group specialises in conducting native MS experiments.<\/p>\n\n\n\n<p>To observe Ent1 and Ent2 preferences in the cellular context, the Garc\u00eda-Alia Team performed experiments at CSSB\u2019s <a href=\"https:\/\/www.cssb-hamburg.de\/facilities\/alfm\/index_eng.html\">Advanced Light and Fluorescence Microscopy (ALFM) Facility<\/a>. As clathrin-mediated endocytosis is such a fast process, the scientists used an inhibitor to slow down the proteins. They tagged Ent1 and Ent2 with fluorescent proteins and then conducted FRET experiments, which report on the close spatial proximity of proteins, to observe their interactions with clathrin\u2019s NTD boxes. \u201cOur results indicate a preferential binding of Ent1 to the Clathrin box and of Ent2 to the Arrestin box,\u201d noted ALFM Facility Head Roland Thuenauer. \u201cWe also noticed that Ent1 demonstrates a stronger interaction with clathrin.\u201d<\/p>\n\n\n\n<p>\u201cAs Ent1 and Ent2 were previously thought to be functionally redundant, these results are very exciting,\u201d stated Defelipe. \u201cEnt1\u2019s stronger bond with clathrin also helps explain its functional divergence towards actin binding. Overall, we have shown that adaptor protein selectivity and its competitive binding of clathrin results in functional specialisation.\u201d<\/p>\n\n\n\n<hr class=\"vf-divider\"\/>\n\n\n\n<p><em>This article has been adapted from a news article by the <\/em><a href=\"https:\/\/www.cssb-hamburg.de\/news_amp_events\/articles\/2024\/structure_and_function_of_new_lysosome_transporter_revealed\/index_eng.html\"><em>Centre for Structural Systems Biology (CSSB)<\/em><\/a><em>. Find the original story at the CSSB website <a href=\"https:\/\/www.cssb-hamburg.de\/news_amp_events\/articles\/2024\/picky_proteins_understanding_yeast_adaptor_protein_selectivity\/index_eng.html\"><strong>here<\/strong><\/a><\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Scientists from EMBL Hamburg and CSSB have revealed key insights into the cellular process of clathrin-mediated endocytosis.<\/p>\n","protected":false},"author":16,"featured_media":71651,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[17591],"tags":[775,11421,53,5768,5752,5744,35],"embl_taxonomy":[9596,19263,19369],"class_list":["post-71649","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-technology","tag-cssb","tag-garcia-alai","tag-hamburg","tag-macromolecular-crystallography","tag-protein-structure","tag-sample-preparation-and-characterisation-facility","tag-structural-biology","embl_taxonomy-embl-hamburg","embl_taxonomy-garcia-alai-team","embl_taxonomy-sample-preparation-and-characterisation-facility"],"acf":{"featured":true,"show_featured_image":false,"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"article_intro":"<p>Key insights into a mechanism via which cells bring in cargo from outside<\/p>\n","related_links":[{"link_description":"Reshaping the cell membrane with molecular pegs","link_url":"https:\/\/www.embl.org\/news\/science\/molecular-pegs\/"}],"source_article":[{"publication_title":"Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast","publication_link":{"title":"","url":"https:\/\/www.nature.com\/articles\/s41467-024-54037-z","target":"_blank"},"publication_authors":"Defelipe L., et al. ","publication_source":"Nature Communications","publication_date":"7 November 2024","publication_doi":"10.1038\/s41467-024-54037-z"}],"in_this_article":false,"press_contact":"None","article_translations":false,"languages":"","vf_locked":false,"vfwp-news_embl_taxonomy":[9596,19263,19369]},"embl_taxonomy_terms":[{"uuid":"a:3:{i:0;s:36:\"b14d3f13-5670-44fb-8970-e54dfd9c921a\";i:1;s:36:\"89e00fee-87f4-482e-a801-4c3548bb6a58\";i:2;s:36:\"613c4de5-1775-447f-af71-4b07085318e9\";}","parents":[],"name":["EMBL Hamburg"],"slug":"embl-hamburg","description":"Where &gt; All EMBL sites &gt; EMBL Hamburg"},{"uuid":"a:3:{i:0;s:36:\"302cfdf7-365b-462a-be65-82c7b783ebf7\";i:1;s:36:\"2dc39890-6c01-47bf-ac78-d42abdb10079\";i:2;s:36:\"679ec226-70c4-4581-97d1-2a6bcf75b516\";}","parents":[],"name":["Garcia Alai Team"],"slug":"garcia-alai-team","description":"What &gt; Structural Biology (EMBL Hamburg) &gt; Garcia Alai Team"},{"uuid":"a:3:{i:0;s:36:\"302cfdf7-365b-462a-be65-82c7b783ebf7\";i:1;s:36:\"42b86629-bf66-44cd-b9e0-61590f8e8584\";i:2;s:36:\"01cca59b-ea0d-4c7d-b627-4cd295f3f9bc\";}","parents":[],"name":["Sample Preparation and Characterisation Facility"],"slug":"sample-preparation-and-characterisation-facility","description":"What &gt; Services at EMBL Hamburg &gt; Sample Preparation and Characterisation Facility"}],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Picky proteins: understanding yeast adaptor protein selectivity | EMBL<\/title>\n<meta name=\"description\" content=\"Scientists from EMBL Hamburg and CSSB have revealed key insights into the cellular process of clathrin-mediated endocytosis.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Picky proteins: understanding yeast adaptor protein selectivity | EMBL\" \/>\n<meta property=\"og:description\" content=\"Scientists from EMBL Hamburg and CSSB have revealed key insights into the cellular process of clathrin-mediated endocytosis.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2024-11-14T09:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-11-25T16:07:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1000\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Guest author(s)\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@embl\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Guest author(s)\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/\"},\"author\":{\"name\":\"Guest author(s)\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/b4d9366b2ebe691c4015c64c3619205b\"},\"headline\":\"Picky proteins: understanding yeast adaptor protein selectivity\",\"datePublished\":\"2024-11-14T09:00:00+00:00\",\"dateModified\":\"2024-11-25T16:07:32+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/\"},\"wordCount\":632,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg\",\"keywords\":[\"cssb\",\"garcia alai\",\"hamburg\",\"macromolecular crystallography\",\"protein structure\",\"sample preparation and characterisation facility\",\"structural biology\"],\"articleSection\":[\"Science &amp; Technology\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/\",\"url\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/\",\"name\":\"Picky proteins: understanding yeast adaptor protein selectivity | EMBL\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg\",\"datePublished\":\"2024-11-14T09:00:00+00:00\",\"dateModified\":\"2024-11-25T16:07:32+00:00\",\"description\":\"Scientists from EMBL Hamburg and CSSB have revealed key insights into the cellular process of clathrin-mediated endocytosis.\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage\",\"url\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg\",\"contentUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg\",\"width\":1000,\"height\":600,\"caption\":\"A closer look at clathrin-coated vesicles during endocytosis, highlighting the N-terminal domain of clathrin (PDB: 1XI4) with three binding sites simultaneously occupied (PDB: 9EXT). Credit: Tatiana Kupriianova\/EMBL, David Goodsell\/Scripps, canva.com\"},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.embl.org\/news\/#website\",\"url\":\"https:\/\/www.embl.org\/news\/\",\"name\":\"European Molecular Biology Laboratory News\",\"description\":\"News from the European Molecular Biology Laboratory\",\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"alternateName\":\"EMBL News\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.embl.org\/news\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.embl.org\/news\/#organization\",\"name\":\"European Molecular Biology Laboratory\",\"alternateName\":\"EMBL\",\"url\":\"https:\/\/www.embl.org\/news\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png\",\"contentUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png\",\"width\":300,\"height\":144,\"caption\":\"European Molecular Biology Laboratory\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/embl.org\/\",\"https:\/\/x.com\/embl\",\"https:\/\/www.instagram.com\/embl_org\/\",\"https:\/\/www.linkedin.com\/company\/15813\/\",\"https:\/\/www.youtube.com\/user\/emblmedia\/\"]},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/b4d9366b2ebe691c4015c64c3619205b\",\"name\":\"Guest author(s)\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/300b9a1d66050ae03eaeb99869c6ebb30f5184b9468e92a2b3e7d28bc9cf742d?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/300b9a1d66050ae03eaeb99869c6ebb30f5184b9468e92a2b3e7d28bc9cf742d?s=96&d=mm&r=g\",\"caption\":\"Guest author(s)\"},\"description\":\"Guest author(s)\",\"url\":\"https:\/\/www.embl.org\/news\/author\/guest-author\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Picky proteins: understanding yeast adaptor protein selectivity | EMBL","description":"Scientists from EMBL Hamburg and CSSB have revealed key insights into the cellular process of clathrin-mediated endocytosis.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/","og_locale":"en_US","og_type":"article","og_title":"Picky proteins: understanding yeast adaptor protein selectivity | EMBL","og_description":"Scientists from EMBL Hamburg and CSSB have revealed key insights into the cellular process of clathrin-mediated endocytosis.","og_url":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/","og_site_name":"EMBL","article_publisher":"https:\/\/www.facebook.com\/embl.org\/","article_published_time":"2024-11-14T09:00:00+00:00","article_modified_time":"2024-11-25T16:07:32+00:00","og_image":[{"width":1000,"height":600,"url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg","type":"image\/jpeg"}],"author":"Guest author(s)","twitter_card":"summary_large_image","twitter_creator":"@embl","twitter_site":"@embl","twitter_misc":{"Written by":"Guest author(s)","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"NewsArticle","@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#article","isPartOf":{"@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/"},"author":{"name":"Guest author(s)","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/b4d9366b2ebe691c4015c64c3619205b"},"headline":"Picky proteins: understanding yeast adaptor protein selectivity","datePublished":"2024-11-14T09:00:00+00:00","dateModified":"2024-11-25T16:07:32+00:00","mainEntityOfPage":{"@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/"},"wordCount":632,"publisher":{"@id":"https:\/\/www.embl.org\/news\/#organization"},"image":{"@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage"},"thumbnailUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg","keywords":["cssb","garcia alai","hamburg","macromolecular crystallography","protein structure","sample preparation and characterisation facility","structural biology"],"articleSection":["Science &amp; Technology"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/","url":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/","name":"Picky proteins: understanding yeast adaptor protein selectivity | EMBL","isPartOf":{"@id":"https:\/\/www.embl.org\/news\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage"},"image":{"@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage"},"thumbnailUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg","datePublished":"2024-11-14T09:00:00+00:00","dateModified":"2024-11-25T16:07:32+00:00","description":"Scientists from EMBL Hamburg and CSSB have revealed key insights into the cellular process of clathrin-mediated endocytosis.","inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/science-technology\/picky-proteins-understanding-yeast-adaptor-protein-selectivity\/#primaryimage","url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg","contentUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg","width":1000,"height":600,"caption":"A closer look at clathrin-coated vesicles during endocytosis, highlighting the N-terminal domain of clathrin (PDB: 1XI4) with three binding sites simultaneously occupied (PDB: 9EXT). Credit: Tatiana Kupriianova\/EMBL, David Goodsell\/Scripps, canva.com"},{"@type":"WebSite","@id":"https:\/\/www.embl.org\/news\/#website","url":"https:\/\/www.embl.org\/news\/","name":"European Molecular Biology Laboratory News","description":"News from the European Molecular Biology Laboratory","publisher":{"@id":"https:\/\/www.embl.org\/news\/#organization"},"alternateName":"EMBL News","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.embl.org\/news\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.embl.org\/news\/#organization","name":"European Molecular Biology Laboratory","alternateName":"EMBL","url":"https:\/\/www.embl.org\/news\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/","url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png","contentUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png","width":300,"height":144,"caption":"European Molecular Biology Laboratory"},"image":{"@id":"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/embl.org\/","https:\/\/x.com\/embl","https:\/\/www.instagram.com\/embl_org\/","https:\/\/www.linkedin.com\/company\/15813\/","https:\/\/www.youtube.com\/user\/emblmedia\/"]},{"@type":"Person","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/b4d9366b2ebe691c4015c64c3619205b","name":"Guest author(s)","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/300b9a1d66050ae03eaeb99869c6ebb30f5184b9468e92a2b3e7d28bc9cf742d?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/300b9a1d66050ae03eaeb99869c6ebb30f5184b9468e92a2b3e7d28bc9cf742d?s=96&d=mm&r=g","caption":"Guest author(s)"},"description":"Guest author(s)","url":"https:\/\/www.embl.org\/news\/author\/guest-author\/"}]}},"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"fimg_url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg","featured_image_src":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2024\/11\/Clathrin_and_adaptors-1000x600-1.jpg","_links":{"self":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/71649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/comments?post=71649"}],"version-history":[{"count":4,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/71649\/revisions"}],"predecessor-version":[{"id":78489,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/71649\/revisions\/78489"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/media\/71651"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/media?parent=71649"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/categories?post=71649"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/tags?post=71649"},{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/embl_taxonomy?post=71649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}