{"id":58933,"date":"2023-05-05T10:00:00","date_gmt":"2023-05-05T08:00:00","guid":{"rendered":"https:\/\/www.embl.org\/news\/?p=58933"},"modified":"2024-03-22T11:16:46","modified_gmt":"2024-03-22T10:16:46","slug":"the-hairy-single-celled-tetrahymena-provides-a-work-reset","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/lab-matters\/the-hairy-single-celled-tetrahymena-provides-a-work-reset\/","title":{"rendered":"The hairy single-celled Tetrahymena provides a work reset"},"content":{"rendered":"\n<p>An \u2018ordinary\u2019 sabbatical might take <em>one <\/em>individual on a personal journey away from the daily grind to recharge batteries so they can return with a new professional perspective.<\/p>\n\n\n\n<p>But why settle for the ordinary when you can do the <em>extra<\/em>ordinary?<\/p>\n\n\n\n<p>That could be the slogan for annual \u2018labbaticals\u2019 \u2013 two of which have now occurred in EMBL\u2019s Dey group. The entire group halts day-to-day activities as much as possible to jointly pursue collective projects for two weeks. They apply their usual high-tech tools and evolutionary cell biology expertise, but with a totally different model organism and a goal of just seeing what happens.<\/p>\n\n\n\n<p>Last year, the Dey group embarked on this research side step using <em>Physarum polycephalum<\/em>, an acellular slime mould. This year, <em>Tetrahymena, <\/em>a free-living ciliated microorganism,<em> <\/em>was the focal point. The goal was to have a \u2018fun refresh\u2019 and possibly jump-start scientific pursuits from this unusual exploration.<\/p>\n\n\n\n<p>\u201cThe idea came from seeing how intensive sabbatical courses around the world have been known to lead to cool discoveries as scientists try a bunch of new, different things,\u201d said Gautam Dey, EMBL Group Leader. \u201cEMBL is a particularly good place for this since we have so many interesting tools and people are always willing to help. Here, if we dream it, we can probably do it.\u201d<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>\u2018Slime mania\u2019 for a refresh<\/strong><\/h2>\n\n\n\n<p>Last spring, armed with a long list of experiment ideas, unbridled enthusiasm, and group \u2018Slime Mania\u2019 t-shirts made especially for the occasion, the Dey group looked to see what they could learn from <em>Physarum<\/em>, which is viewed as a sort of cellular computer, despite its blob-like, slime mould appearance.<\/p>\n\n\n\n<p>\u201cIt was a very popular model system in the \u201860s and \u201870s, with a revival in recent years as people became interested in cellular computation,\u201d Dey said. \u201cThere are some <a href=\"https:\/\/www.pnas.org\/doi\/10.1073\/pnas.0912198107#:~:text=Species.-,P.,bacteria%20and%20dead%20organic%20matter\">really cool experiments where scientists give <em>Physarum <\/em>multiple food sources on different parts of a plate<\/a>, and the <em>Physarum <\/em>makes decisions about which one it prefers to eat, yet it\u2019s just one cell.\u201d<\/p>\n\n\n\n<p>Despite its long-standing involvement in biological research, many unanswered basic cell biology questions about <em>Physarum<\/em> persist, such as how it divides and what triggers the switch between stages of its complex life cycle. New microscopy and other tools available at EMBL would likely help provide clearer answers.<\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-full is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"1182\" height=\"1414\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/2.-f_mikus.jpg\" alt=\"A pinkish-white sphere is coloured by shades of red and purple to indicate the passage of time.\" class=\"wp-image-58943\" srcset=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/2.-f_mikus.jpg 1182w, https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/2.-f_mikus-251x300.jpg 251w, https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/2.-f_mikus-856x1024.jpg 856w, https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/2.-f_mikus-768x919.jpg 768w\" sizes=\"auto, (max-width: 1182px) 100vw, 1182px\" \/><figcaption class=\"vf-figure__caption\">Felix Mikus won the British Society for Cell Biology\u2019s image competition in 2022 with this time series projection of the slime mould <em>Physarum polycephalum<\/em> (a.k.a. The Blob) colonising an agar plate. With its thousands of nuclei sharing a single cytoplasm, it &#8216;expanded&#8217; at the rate of centimetres per hour, making it large enough to be photographed with his mobile phone. Credit: Felix Mikus\/EMBL<br \/><\/figcaption><\/figure>\n\n\n\n<p>While the group only completed a small fraction of its intended experiments, the experience grew into two collaborative projects that new Dey group postdocs are now leading. It also prompted the team to link up with others interested in <em>Physarum<\/em> cell biology around the world, including the labs of Amy Gladfelter (Duke) and Karen Alim (TU Munich). Additionally, Felix Mikus, who was the group\u2019s first PhD student to join in 2021, won the <a href=\"https:\/\/bscb.org\/image-competition-2022\/\">British Society for Cell Biology\u2019s image competition in 2022 <\/a>with his <em>Physarum<\/em> image taken without a microscope and with only a mobile phone.&nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Bring on the <\/strong><strong><em>Tetrahymena<\/em><\/strong><\/h2>\n\n\n\n<p>For this year\u2019s \u2018labbatical\u2019, <em>Tetrahymena <\/em>seemed like a good choice. The Dey group is involved in EMBL\u2019s <a href=\"https:\/\/www.embl.org\/about\/info\/trec\/\">TREC expedition<\/a> doing field sampling along Europe\u2019s coastlines for plankton, and many of their samples are full of ciliated organisms like <em>Tetrahymena<\/em>.&nbsp;<\/p>\n\n\n\n<p>\u201c<em>Tetrahymena<\/em> has very weird biology, but it&#8217;s everywhere,\u201d Dey said. \u201cThey have many hair-like cilia on their body and use those for swimming, feeding, and so on. It\u2019s also been responsible for two Nobel prizes.\u201d<\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-full is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"960\" height=\"960\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/MAX_Image-3_Airyscan-Processing.png\" alt=\"A magenta circle is surrounded by grey cobweb-like material against a black background\" class=\"wp-image-58945\" srcset=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/MAX_Image-3_Airyscan-Processing.png 960w, https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/MAX_Image-3_Airyscan-Processing-300x300.png 300w, https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/MAX_Image-3_Airyscan-Processing-150x150.png 150w, https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/MAX_Image-3_Airyscan-Processing-768x768.png 768w\" sizes=\"auto, (max-width: 960px) 100vw, 960px\" \/><figcaption class=\"vf-figure__caption\">Using expansion microscopy, the scientists captured images like this one of the <em>Tetrahymena<\/em>. Here is an expanded <em>Tetrahymena thermophila<\/em> cell with its tubulin shown in grey and DNA in magenta.&nbsp; Credit: Felix Mikus\/EMBL&nbsp;<\/figcaption><\/figure>\n\n\n\n<p><em>Tetrahymena <\/em>were critical to discovering telomeres (the ends of chromosomes) and their role in ageing and replication. Interestingly, it has two nuclei. A tiny nucleus silently copies the genome and passes it on through sexual reproduction. Additionally, a mega nucleus contains many copies of the genome that it uses to grow and divide. The mega nucleus has thousands of mini chromosomes, so it has thousands of telomeres. That&#8217;s how Nobel Laureate Elizabeth Blackburn was able to make her finding.&nbsp; Additionally, Thomas Cech used <em>Tetrahymena <\/em>in his Nobel-recognised discovery that RNA can splice itself, possibly indicating that life might have started as RNA.&nbsp;<\/p>\n\n\n\n<p><em>Tetrahymena\u2019s<\/em> long history as a model system meant it was easy for the Dey group to obtain the cells (in this case, a gift from Geert Kops at the University of Utrecht, and stocks ordered from the Tetrahymena Stock Center at Cornell University).<\/p>\n\n\n\n<p>\u201cThis year, we prepared more ahead of time \u2013 and perhaps more realistically \u2013 but we still only managed to do about 15% of the experiments we planned,\u201d Dey said.&nbsp; \u201cWe got some very nice images with a technique we&#8217;ve used a lot in the lab called expansion microscopy. While we know of other labs applying expansion microscopy to <em>Tetrahymena<\/em>, these studies are yet to be published.\u201d<\/p>\n\n\n\n<p>Mikus, in fact, will be one of the instructors in the inaugural edition of EMBL\u2019s <a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/events\/eic23-02\/\">expansion microscopy course<\/a> this summer.&nbsp;<\/p>\n\n\n\n<p>\u201cWe tried some live imaging, which was very difficult because <em>Tetrahymena <\/em>swim very fast and they don&#8217;t like being immobilised, which we knew from the literature,\u201d Mikus said. \u201cThey also don&#8217;t like being illuminated, which is not ideal for light microscopy.\u201d<\/p>\n\n\n\n<p>This year, with additional electron microscopy expertise from the Schwab team, the \u2018labbatical\u2019 discovered some features of <em>Tetrahymena <\/em>mitosis that had not been systematically reported in scientific literature.<\/p>\n\n\n\n<p>Clearly, \u2018labbaticals\u2019 are catching on. The Dorrity group, which normally works with zebrafish to understand environmental impacts and responses at a cellular level, temporarily switched to sturgeon for their own recent group-wide break from day-to-day research.&nbsp;<\/p>\n\n\n\n<p>\u201cSo now we have a little bit of expertise in this area, some of the tools, and some knowledge of what works and doesn&#8217;t,\u201d Dey said. \u201cIn two weeks, you obviously can\u2019t even get close to finishing a piece of science, but you can open many doors.\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Dey Group holds second annual \u2018labbatical\u2019 to step outside daily research tasks with the help of single-celled model organisms.<\/p>\n","protected":false},"author":100,"featured_media":58941,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3,17591],"tags":[64,474,13676,836],"embl_taxonomy":[19201],"class_list":["post-58933","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-matters","category-science-technology","tag-cell-biology","tag-collaboration","tag-dey","tag-lab","embl_taxonomy-dey-group"],"acf":{"featured":true,"show_featured_image":false,"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"article_intro":"<p>Dey Group holds second annual \u2018labbatical\u2019 to step outside daily research tasks with the help of single-celled model organisms<\/p>\n","related_links":[{"link_description":"Dey group","link_url":"https:\/\/www.embl.org\/groups\/dey\/"},{"link_description":"Cell Biology and Biophysics unit","link_url":"https:\/\/www.embl.org\/research\/units\/cell-biology-biophysics\/"}],"source_article":false,"in_this_article":false,"press_contact":"None","article_translations":false,"languages":""},"embl_taxonomy_terms":[{"uuid":"a:3:{i:0;s:36:\"302cfdf7-365b-462a-be65-82c7b783ebf7\";i:1;s:36:\"64999cc4-9a7c-4fea-8339-0e2acc990e08\";i:2;s:36:\"af4ad533-c1c7-43aa-b7a7-1336c1394406\";}","parents":[],"name":["Dey Group"],"slug":"dey-group","description":"What &gt; Cell biology and biophysics &gt; Dey Group"}],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>The hairy single-celled Tetrahymena provides a work reset | EMBL<\/title>\n<meta name=\"description\" content=\"Dey Group holds second annual \u2018labbatical\u2019 to step outside daily research tasks with the help of single-celled model organisms\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/lab-matters\/the-hairy-single-celled-tetrahymena-provides-a-work-reset\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The hairy single-celled Tetrahymena provides a work reset | EMBL\" \/>\n<meta property=\"og:description\" content=\"Dey Group holds second annual \u2018labbatical\u2019 to step outside daily research tasks with the help of single-celled model organisms\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/lab-matters\/the-hairy-single-celled-tetrahymena-provides-a-work-reset\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2023-05-05T08:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-03-22T10:16:46+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/Labbatical-Dey-Group-2023-wp.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1000\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Ivy Kupec\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@embl\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Ivy Kupec\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/lab-matters\/the-hairy-single-celled-tetrahymena-provides-a-work-reset\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/lab-matters\/the-hairy-single-celled-tetrahymena-provides-a-work-reset\/\"},\"author\":{\"name\":\"Ivy Kupec\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/427f2c9b624bc32ffa67d80414712274\"},\"headline\":\"The hairy single-celled Tetrahymena provides a work reset\",\"datePublished\":\"2023-05-05T08:00:00+00:00\",\"dateModified\":\"2024-03-22T10:16:46+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/lab-matters\/the-hairy-single-celled-tetrahymena-provides-a-work-reset\/\"},\"wordCount\":1066,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/lab-matters\/the-hairy-single-celled-tetrahymena-provides-a-work-reset\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2023\/05\/Labbatical-Dey-Group-2023-wp.jpg\",\"keywords\":[\"cell biology\",\"collaboration\",\"dey\",\"lab\"],\"articleSection\":[\"Lab Matters\",\"Science &amp; 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