{"id":5575,"date":"2015-10-30T19:00:35","date_gmt":"2015-10-30T18:00:35","guid":{"rendered":"http:\/\/news.embl.de\/?p=5575"},"modified":"2024-04-19T15:42:44","modified_gmt":"2024-04-19T13:42:44","slug":"1510-vesicles","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/","title":{"rendered":"One hard pull"},"content":{"rendered":"\n

In a paper published today in PLoS Computational Biology<\/em>, postdoctoral fellow Serge Dmitrieff used mechanical equilibrium theory to predict the force needed to overcome a yeast cell\u2019s internal pressure and bend its membrane inwards. Remarkably, his calculations show that the actin fibres have to exert a force that\u2019s 2500 times the cell\u2019s own weight. The approach also enabled the EMBL scientists to determine, on the computer, which elements of the cellular machinery are indispensable for the task. They found that after that initial, harder-than-expected pull, the membrane is unlikely to stop bending until the vesicle is formed. Dmitrieff and N\u00e9d\u00e9lec also discovered that the neck of the budding vesicle doesn\u2019t have to be \u2018tied off\u2019 by actin fibres to release the vesicle into the cell \u2013 removing a set of crescent-shaped proteins from the bud\u2019s base is enough to do the trick.<\/p>\n\n\n\n

The study builds on previous work in which John Briggs<\/a>\u2019 and Marko Kaksonen<\/a>\u2019s groups combined two microscopy techniques to follow changes in the shape of the cell\u2019s membrane and track proteins<\/a> thought to influence those changes.<\/p>\n","protected":false},"excerpt":{"rendered":"

Fibres that pull membrane to form a vesicle exert a force that\u2019s 2500 times a yeast cell\u2019s own weight<\/p>\n","protected":false},"author":8,"featured_media":5591,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[2,17591],"tags":[65,348,64,43,57],"embl_taxonomy":[],"class_list":["post-5575","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science","category-science-technology","tag-biophysics","tag-briggs","tag-cell-biology","tag-heidelberg","tag-postdoc"],"acf":{"article_intro":"

In yeast cells, the network of actin fibres that pulls the membrane inwards to form a vesicle has to pull harder than scientists thought, Fran\u00e7ois N\u00e9d\u00e9lec\u2019s group<\/a> in Heidelberg have shown.<\/p>\n","related_links":[{"link_description":"Kaksonen and N\u00e9d\u00e9lec combined 3 kinds of microscopy to study vesicle formation","link_url":"http:\/\/news.embl.de\/science\/1502_endocytosis\/"},{"link_description":"Recent work on this topic by the Briggs group","link_url":"http:\/\/news.embl.de\/science\/1506-clathrin\/"}],"article_sources":[{"source_description":"

Dmitrieff, S. & N\u00e9d\u00e9lec, F. PLoS Computational Biology<\/em>, 30 October 2015. DOI:\u00a010.1371\/journal.pcbi.1004538<\/p>\n","source_link_url":"http:\/\/dx.doi.org\/10.1371\/journal.pcbi.1004538"}],"vf_locked":false,"featured":false,"color":"#007B53"},"embl_taxonomy_terms":[],"yoast_head":"\nOne hard pull | EMBL<\/title>\n<meta name=\"description\" content=\"Fibres that pull membrane to form a vesicle exert a force that\u2019s 2500 times a yeast cell\u2019s own weight\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"One hard pull | EMBL\" \/>\n<meta property=\"og:description\" content=\"Fibres that pull membrane to form a vesicle exert a force that\u2019s 2500 times a yeast cell\u2019s own weight\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2015-10-30T18:00:35+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-04-19T13:42:44+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/10\/1510-pull-ib.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"620\" \/>\n\t<meta property=\"og:image:height\" content=\"425\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Sonia Furtado Neves\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@Aur_ora\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Sonia Furtado Neves\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/\"},\"author\":{\"name\":\"Sonia Furtado Neves\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/d926199a955624b44dda296f396c5e68\"},\"headline\":\"One hard pull\",\"datePublished\":\"2015-10-30T18:00:35+00:00\",\"dateModified\":\"2024-04-19T13:42:44+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/\"},\"wordCount\":188,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/10\/1510-pull-ib.jpg\",\"keywords\":[\"biophysics\",\"briggs\",\"cell biology\",\"heidelberg\",\"postdoc\"],\"articleSection\":[\"Science\",\"Science & Technology\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/\",\"url\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/\",\"name\":\"One hard pull | EMBL\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/10\/1510-pull-ib.jpg\",\"datePublished\":\"2015-10-30T18:00:35+00:00\",\"dateModified\":\"2024-04-19T13:42:44+00:00\",\"description\":\"Fibres that pull membrane to form a vesicle exert a force that\u2019s 2500 times a yeast cell\u2019s own weight\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/1510-vesicles\/#primaryimage\",\"url\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/10\/1510-pull-ib.jpg\",\"contentUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/10\/1510-pull-ib.jpg\",\"width\":620,\"height\":425,\"caption\":\"Pulling a membrane inwards is harder than scientists first thought. 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