{"id":5066,"date":"2015-08-26T11:21:12","date_gmt":"2015-08-26T09:21:12","guid":{"rendered":"http:\/\/news.embl.de\/?p=5066"},"modified":"2024-04-19T15:15:08","modified_gmt":"2024-04-19T13:15:08","slug":"1508_super-impressions","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/events\/1508_super-impressions\/","title":{"rendered":"Super impressions"},"content":{"rendered":"\n

Super-resolution microscopy pushes the boundaries of conventional microscopes, enabling scientists to visualise structures with higher resolution than ever before, increasing our understanding of important molecular pathways inside living cells \u2013 a feat that earned its developers the 2014 Nobel Prize in Chemistry<\/a>.<\/p>\n\n\n\n

Over six days, course participants covered theory and practical applications, gaining hands-on experience with the latest commercially available super-resolution microscopes<\/a> for STED and GSDIM localisation, alongside in-depth tuition and talks from more than a dozen renowned guest researchers and experts from EMBL and Leica Microsystems. Towards the end of their busy week, we asked a few of the participants for their impressions.<\/p>\n\n\n\n

What\u2019s one thing you\u2019ll definitely be taking back with you to your lab?<\/h3>\n\n\n\n

\u201cThere are certainly some techniques that I really wanted to try, and here I got to ask about technical details and see how things work in practice. I came in with my particular set of preferences, what I liked and didn\u2019t like, but now this course is changing my mind, or widening my perspectives.\u201d Vaclav Beranek, MRC Laboratory of Molecular Biology, UK<\/strong><\/p>\n\n\n\n

This course is changing my mind, or widening my perspectives.<\/p><\/blockquote>\n\n\n\n

\u201cThere\u2019s one fluorophore that I could never get to work in my lab, labelling kidney tissues. I got a great tip that you should use really, really low depletion power for that fluorophore \u2013 now I know it wasn\u2019t the fluorophore itself, it was us, and I can go back and try it again.\u201d David Unnersjoe-Jess, Royal Institute of Technology, Sweden<\/strong><\/p>\n\n\n\n

\u201cThe teaching, particularly the microscope sessions. Normally someone shows you once during training, and then you\u2019re on your own \u2013 the first time you use the microscope, you\u2019re looking around, trying to remember \u2018where did they click?\u2019. Here, with the trainer beside you, you work in small groups and gain a lot more confidence on the system. It\u2019s a lot more memorable.\u201d Ella Fung, University of Oxford, UK<\/strong><\/p>\n\n\n\n

\"Ella
Ella Fung during a microscopy session. PHOTO: EMBL Photolab\/Marietta Schupp<\/figcaption><\/figure><\/div>\n\n\n\n

What has this course added to your wish list?<\/h3>\n\n\n\n

\u201cI would like to have all these people \u2013 organisers and speakers \u2013 as my collaborators, that would be fantastic! Then we could use all of these tools they have developed.\u201d Vaclav Beranek<\/strong><\/p>\n\n\n\n

\u201cI wish that our institute had something like the Advanced Light Microscopy Core Facility. The set up here is amazing \u2013 to have it in house, buzzing with people who can help and give you cool ideas. My wish list: Marko Lampe and his microscopes!\u201d Ella Fung<\/strong><\/p>\n\n\n\n

What was the coolest application you learned of?<\/h3>\n\n\n\n

\u201cThere was a talk by Katrin Willig about a really cool application of STED microscopy, where they actually looked at the brain of a living mouse<\/a>. I was really impressed by how deep they could go and still keep the resolution.\u201d David Unnersjoe-Jess<\/strong><\/p>\n\n\n\n

As a PhD student, I could see what was achievable.<\/p><\/blockquote>\n\n\n\n

\u201cI really liked what Christian Eggeling has done, because I think he uses the technique where it provides really interesting new biological knowledge and not just beautiful images. Eggeling studies lipids with these super-resolution techniques<\/a>, and it seems like a really powerful tool for that.\u201d Vaclav Beranek<\/strong><\/p>\n\n\n\n

\u201cGroup leader Jan Ellenberg presented <\/span>a PhD student\u2019s project<\/a> using these techniques to visualize the structure of a molecular complex. As a PhD student, I could see what was achievable and also appreciate how much work she had done \u2013 I particularly liked that he gave the timescale of the project, and told us what difficulties they had.\u201d Ella Fung<\/strong><\/p>\n\n\n\n

Is there anything people really have to know about this course?<\/h3>\n\n\n\n

\u201cIf you need a technique that no-one in your institute knows or can help with \u2013especially if it\u2019s a rapidly developing technique and you alone can\u2019t keep up with the literature \u2013 then this is a great course to attend: it\u2019s like living a review!\u201d Ella Fung<\/strong><\/p>\n\n\n\n

It\u2019s like living a review!<\/p><\/blockquote>\n\n\n\n

\"Participants
Participants from around the world joined experts in the field at EMBL Heidelberg. PHOTO: EMBL Photolab\/Marietta Schupp<\/figcaption><\/figure><\/div>\n","protected":false},"excerpt":{"rendered":"

“It’s like living a review!” Participants of recent super-resolution microscopy course share their highlights<\/p>\n","protected":false},"author":8,"featured_media":5080,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[17595,4],"tags":[302,43,69,79,27],"embl_taxonomy":[],"class_list":["post-5066","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-connections","category-events","tag-ellenberg","tag-heidelberg","tag-methods","tag-microscopy","tag-training"],"acf":{"article_intro":"

From tips for using tricky fluorophores to an understanding of how long a project can take, there was something for everyone at the EMBL Advanced Course Super-Resolution Microscopy last month. Now in its second edition, the course is jointly organised by the EMBL Advanced Light Microscopy Core Facility<\/a> and corporate partner Leica Microsystems<\/a>.<\/p>\n","related_links":[{"link_description":"Be among the first to learn the date of the next Advanced Course Super-Resolution Microscopy: sign up to the quarterly events e-newsletter ","link_url":"http:\/\/www.embl.de\/training\/events\/info_participants\/information\/index.html"},{"link_description":"Learn more about super-resolution microscopy on Science Lab ","link_url":"http:\/\/www.leica-microsystems.com\/science-lab\/topics\/super-resolution\/"},{"link_description":"Interview with Anna Szymborska and Jan Ellenberg on Science Lab","link_url":"http:\/\/www.leica-microsystems.com\/science-lab\/super-resolution-microscopy-gives-new-insights-into-nuclear-pore-complex-organization\/"},{"link_description":"Webinar: Applications and Methodology of Super-Resolution Microscopy with Leica SR GSD 3D","link_url":"http:\/\/www.leica-microsystems.com\/science-lab\/webinar-applications-and-methodology-of-super-resolution-microscopy-with-leica-sr-gsd-3d\/"},{"link_description":"Webinar on demand: Unleashing the Powers of Super-Resolution Microscopy to Solve Immunological Challenges","link_url":"http:\/\/www.leica-microsystems.com\/science-lab\/webinar-unleashing-the-powers-of-super-resolution-microscopy-to-solve-immunological-challenges\/"},{"link_description":"Leica Super-Resolution ","link_url":"http:\/\/www.leica-microsystems.com\/produkte\/super-resolution\/"},{"link_description":"EMBL Corporate Partnership Programme","link_url":"http:\/\/www.embl.de\/aboutus\/resource_dev\/cpp\/index.html"},{"link_description":"See the programme and details of organisers, speakers and trainers for this year\u2019s EMBL Advanced Course: Super-Resolution Microscopy","link_url":"http:\/\/www.embl.de\/training\/events\/2015\/MIC15-03\/index.html"},{"link_description":"Courses and conferences at EMBL","link_url":"http:\/\/www.embl.de\/training\/events\/index.php"}],"article_sources":false,"vf_locked":false,"featured":false,"color":"#007B53"},"embl_taxonomy_terms":[],"yoast_head":"\nSuper impressions | EMBL<\/title>\n<meta name=\"description\" content=\"From tips for tricky fluorophores to understanding how long a project can take, there was something for everyone at our Super-Resolution Microscopy course.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/events\/1508_super-impressions\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Super impressions | EMBL\" \/>\n<meta property=\"og:description\" content=\"From tips for tricky fluorophores to understanding how long a project can take, there was something for everyone at our Super-Resolution 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