{"id":4551,"date":"2015-07-10T02:30:57","date_gmt":"2015-07-10T00:30:57","guid":{"rendered":"http:\/\/news.embl.de\/?p=4551"},"modified":"2024-06-26T14:23:22","modified_gmt":"2024-06-26T12:23:22","slug":"1507-copi","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/science\/1507-copi\/","title":{"rendered":"Cell machinery wears complex coat"},"content":{"rendered":"\n<p>Vesicles are responsible for transporting molecules between the different compartments within a cell and also for bringing material into cells from outside. There are several types of vesicle: each has a specific type of coat which is made up of different proteins and assembles onto a membrane surrounding the vesicle.<\/p>\n\n\n\n<p>The EMBL team has been taking a close look at a coat called COPI. This surrounds vesicles that move material around within the Golgi apparatus and to the endoplasmic reticulum (ER) \u2013 these are regions of the cell where proteins are made and modified in preparation for transport to the cell surface.<\/p>\n\n\n\n<p>Using a technique called cryo-electron tomography, in which samples can be frozen at very low temperatures to avoid fixing or staining them chemically, the researchers combined data from hundreds of vesicles to build up a 3D image of the COPI coat. This enabled them to produce the most detailed pictures yet obtained of a vesicle coat assembled on the vesicle membrane.<\/p>\n\n\n\n<p>This imaging technique is still in its infancy and although scientists have been able to gather structural information about parts of the membrane coats in other types of vesicle, this is the first time a model of a complete assembled vesicle coat has been produced.<\/p>\n\n\n\n<blockquote class=\"vf-blockquote\"><p>This is an important step forward for our understanding of intracellular transportation<\/p><\/blockquote>\n\n\n\n<p>What the images revealed was surprising: unlike other types of vesicle, where the coat is thought to be made from proteins assembled in different layers \u2013&nbsp;each with a specific function \u2013&nbsp;around the vesicle membrane, the EMBL team observed that the proteins in the COPI coat all intertwine together in one big layer, which is curved to fit around the membrane. More precisely, the COPI coat is made of a repetition of building blocks, called &#8216;triads&#8217;, that contain all the important functional elements organised in a precise 3D structure.<\/p>\n\n\n\n<p>\u201cUntil now we could see different elements of the vesicle coat, but not get a complete and detailed picture of the coat assembled onto the vesicle membrane. This is an important step forward for our understanding of intracellular transportation,\u201d explains <a href=\"http:\/\/www.embl.de\/research\/units\/scb\/briggs\/index.html\" target=\"_blank\" rel=\"noopener noreferrer\">John Briggs<\/a>&nbsp;who led the study.<\/p>\n\n\n\n<p>\u201cOur images showed us how the proteins that make up the coat are arranged and it was surprising to discover how different COPI is from, for example, clathrin or COPII coated vesicles,\u201d adds Svetlana Dodonova, co-author of the paper. \u201cOur next step will be to try to find out how this coat forms and binds to the vesicle membrane and how it arranges itself into such complex shapes.\u201d<\/p>\n\n\n\n<p>The research was funded by the German Research Foundation, and carried out in collaboration with Felix Wieland at the University of Heidelberg.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>3D imaging unravels COPI coat of cells&#8217; transport vesicles.<\/p>\n","protected":false},"author":25,"featured_media":4554,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[2,17591],"tags":[64,13958,43,1748,35],"embl_taxonomy":[],"class_list":["post-4551","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science","category-science-technology","tag-cell-biology","tag-dodonova","tag-heidelberg","tag-press-release","tag-structural-biology"],"acf":{"article_intro":"<p>Researchers at EMBL Heidelberg have produced detailed images of the intricate protein-coats that surround trafficking vesicles \u2013\u00a0the &#8216;transport pods&#8217; that move material around within biological cells. The study, published today in <em>Science,<\/em> provides a new understanding of the complex machines that make up the cells\u2019 logistics network.<\/p>\n","related_links":[{"link_description":"","link_url":""},{"link_description":"","link_url":""}],"article_sources":[{"source_description":"<p>Dodonova, Diestelkoetter-Bachert, von Appen <i>et al. Science<\/i>, 10 July 2015. DOI:10.1126\/science.aab1121<\/p>\n","source_link_url":"http:\/\/dx.doi.org\/10.1126\/science.aab1121"}],"vf_locked":false,"featured":false,"color":"#007B53","show_featured_image":false,"in_this_article":false,"youtube_url":"","mp4_url":"","video_caption":"","translations":false,"press_contact":"EMBL Generic","field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"source_article":false,"article_translations":false,"languages":""},"embl_taxonomy_terms":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Cell machinery wears complex coat | EMBL<\/title>\n<meta name=\"description\" content=\"3D imaging unravels COPI coat of cells&#039; transport vesicles.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/science\/1507-copi\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cell machinery wears complex coat | EMBL\" \/>\n<meta property=\"og:description\" content=\"3D imaging unravels COPI coat of cells&#039; transport vesicles.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/science\/1507-copi\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2015-07-10T00:30:57+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-06-26T12:23:22+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"620\" \/>\n\t<meta property=\"og:image:height\" content=\"425\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Isabelle Kling\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@embl\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Isabelle Kling\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/\"},\"author\":{\"name\":\"Isabelle Kling\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/e0df3d2a7d51e01a8118c494997b85c3\"},\"headline\":\"Cell machinery wears complex coat\",\"datePublished\":\"2015-07-10T00:30:57+00:00\",\"dateModified\":\"2024-06-26T12:23:22+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/\"},\"wordCount\":451,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg\",\"keywords\":[\"cell biology\",\"dodonova\",\"heidelberg\",\"press release\",\"structural biology\"],\"articleSection\":[\"Science\",\"Science &amp; Technology\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/\",\"url\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/\",\"name\":\"Cell machinery wears complex coat | EMBL\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg\",\"datePublished\":\"2015-07-10T00:30:57+00:00\",\"dateModified\":\"2024-06-26T12:23:22+00:00\",\"description\":\"3D imaging unravels COPI coat of cells' transport vesicles.\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.embl.org\/news\/science\/1507-copi\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage\",\"url\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg\",\"contentUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg\",\"width\":620,\"height\":425,\"caption\":\"Left: A 'triad', the building block of the COPI coat. Right: COPI coated vesicle made of an assembly of triads. IMAGE: Svetlana Dodonova\/EMBL\"},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.embl.org\/news\/#website\",\"url\":\"https:\/\/www.embl.org\/news\/\",\"name\":\"European Molecular Biology Laboratory News\",\"description\":\"News from the European Molecular Biology Laboratory\",\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"alternateName\":\"EMBL News\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.embl.org\/news\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.embl.org\/news\/#organization\",\"name\":\"European Molecular Biology Laboratory\",\"alternateName\":\"EMBL\",\"url\":\"https:\/\/www.embl.org\/news\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png\",\"contentUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png\",\"width\":300,\"height\":144,\"caption\":\"European Molecular Biology Laboratory\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/embl.org\/\",\"https:\/\/x.com\/embl\",\"https:\/\/www.instagram.com\/embl_org\/\",\"https:\/\/www.linkedin.com\/company\/15813\/\",\"https:\/\/www.youtube.com\/user\/emblmedia\/\"]},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/e0df3d2a7d51e01a8118c494997b85c3\",\"name\":\"Isabelle Kling\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/b702cee3d1a2d162659a8c12aef6045791a912db4ebed961b437edb34dae8cee?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/b702cee3d1a2d162659a8c12aef6045791a912db4ebed961b437edb34dae8cee?s=96&d=mm&r=g\",\"caption\":\"Isabelle Kling\"},\"description\":\"Isabelle Kling trained as a biochemist and science communicator, before setting up various science communication projects in Canada and in Europe. She is now a communication officer with EMBL.\",\"url\":\"https:\/\/www.embl.org\/news\/author\/isabelle-kling\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Cell machinery wears complex coat | EMBL","description":"3D imaging unravels COPI coat of cells' transport vesicles.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.embl.org\/news\/science\/1507-copi\/","og_locale":"en_US","og_type":"article","og_title":"Cell machinery wears complex coat | EMBL","og_description":"3D imaging unravels COPI coat of cells' transport vesicles.","og_url":"https:\/\/www.embl.org\/news\/science\/1507-copi\/","og_site_name":"EMBL","article_publisher":"https:\/\/www.facebook.com\/embl.org\/","article_published_time":"2015-07-10T00:30:57+00:00","article_modified_time":"2024-06-26T12:23:22+00:00","og_image":[{"width":620,"height":425,"url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg","type":"image\/jpeg"}],"author":"Isabelle Kling","twitter_card":"summary_large_image","twitter_creator":"@embl","twitter_site":"@embl","twitter_misc":{"Written by":"Isabelle Kling","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"NewsArticle","@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/#article","isPartOf":{"@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/"},"author":{"name":"Isabelle Kling","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/e0df3d2a7d51e01a8118c494997b85c3"},"headline":"Cell machinery wears complex coat","datePublished":"2015-07-10T00:30:57+00:00","dateModified":"2024-06-26T12:23:22+00:00","mainEntityOfPage":{"@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/"},"wordCount":451,"publisher":{"@id":"https:\/\/www.embl.org\/news\/#organization"},"image":{"@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage"},"thumbnailUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg","keywords":["cell biology","dodonova","heidelberg","press release","structural biology"],"articleSection":["Science","Science &amp; Technology"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/","url":"https:\/\/www.embl.org\/news\/science\/1507-copi\/","name":"Cell machinery wears complex coat | EMBL","isPartOf":{"@id":"https:\/\/www.embl.org\/news\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage"},"image":{"@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage"},"thumbnailUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg","datePublished":"2015-07-10T00:30:57+00:00","dateModified":"2024-06-26T12:23:22+00:00","description":"3D imaging unravels COPI coat of cells' transport vesicles.","inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.embl.org\/news\/science\/1507-copi\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/science\/1507-copi\/#primaryimage","url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg","contentUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg","width":620,"height":425,"caption":"Left: A 'triad', the building block of the COPI coat. Right: COPI coated vesicle made of an assembly of triads. IMAGE: Svetlana Dodonova\/EMBL"},{"@type":"WebSite","@id":"https:\/\/www.embl.org\/news\/#website","url":"https:\/\/www.embl.org\/news\/","name":"European Molecular Biology Laboratory News","description":"News from the European Molecular Biology Laboratory","publisher":{"@id":"https:\/\/www.embl.org\/news\/#organization"},"alternateName":"EMBL News","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.embl.org\/news\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.embl.org\/news\/#organization","name":"European Molecular Biology Laboratory","alternateName":"EMBL","url":"https:\/\/www.embl.org\/news\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/","url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png","contentUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png","width":300,"height":144,"caption":"European Molecular Biology Laboratory"},"image":{"@id":"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/embl.org\/","https:\/\/x.com\/embl","https:\/\/www.instagram.com\/embl_org\/","https:\/\/www.linkedin.com\/company\/15813\/","https:\/\/www.youtube.com\/user\/emblmedia\/"]},{"@type":"Person","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/e0df3d2a7d51e01a8118c494997b85c3","name":"Isabelle Kling","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/b702cee3d1a2d162659a8c12aef6045791a912db4ebed961b437edb34dae8cee?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/b702cee3d1a2d162659a8c12aef6045791a912db4ebed961b437edb34dae8cee?s=96&d=mm&r=g","caption":"Isabelle Kling"},"description":"Isabelle Kling trained as a biochemist and science communicator, before setting up various science communication projects in Canada and in Europe. She is now a communication officer with EMBL.","url":"https:\/\/www.embl.org\/news\/author\/isabelle-kling\/"}]}},"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"fimg_url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg","featured_image_src":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2015\/07\/1507-briggs-ib.jpg","_links":{"self":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/4551","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/users\/25"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/comments?post=4551"}],"version-history":[{"count":15,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/4551\/revisions"}],"predecessor-version":[{"id":24422,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/4551\/revisions\/24422"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/media\/4554"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/media?parent=4551"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/categories?post=4551"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/tags?post=4551"},{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/embl_taxonomy?post=4551"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}