{"id":43603,"date":"2021-10-25T10:00:00","date_gmt":"2021-10-25T08:00:00","guid":{"rendered":"https:\/\/www.embl.org\/news\/?p=43603"},"modified":"2026-02-16T14:16:40","modified_gmt":"2026-02-16T13:16:40","slug":"biophysics-analysis-made-easy-with-an-online-tool","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/science\/biophysics-analysis-made-easy-with-an-online-tool\/","title":{"rendered":"Biophysics analysis made easy with an online tool"},"content":{"rendered":"\n<article class=\"vf-card vf-card--brand vf-card--bordered vf-u-margin__bottom--800\" default>\n  <div class=\"vf-card__content | vf-stack vf-stack--400\">\n      <h3 class=\"vf-card__heading\">\n      Summary    <\/h3>\n                <p class=\"vf-card__text\"><ul>\r\n \t<li>EMBL Hamburg\u2019s Garc\u00eda Alai Team has released <a class=\"vf-card_link\" href=\"https:\/\/spc.embl-hamburg.de\/\">eSPC<\/a>, a freely available online platform for analysing molecular biophysics data from a range of experimental techniques.<\/li>\r\n \t<li>The tool enables scientists around the world to easily analyse their data without the need to travel to the laboratory where the data was generated.<\/li>\r\n<\/ul><\/p>\n      <\/div>\n<\/article>\n\n\n\n\n<p>All biological processes rely on interactions between molecules, such as proteins. Understanding the biophysical aspects of these interactions \u2013 for example, how strong and dynamic they are, and how they change in different conditions \u2013 is extremely helpful in many research fields, including structural biology, biomedicine, and biotechnology. Biophysical analyses can, for example, help to identify drug candidates that are most effective in binding their target.<\/p>\n\n\n\n<p>Scientists perform biophysical analyses at dedicated facilities, such as the <a href=\"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/\">Sample Preparation and Characterisation (SPC) Facility<\/a> at EMBL Hamburg and the <a href=\"https:\/\/www.cssb-hamburg.de\/\">Centre for Structural Systems Biology (CSSB)<\/a>. The SPC Facility offers a pipeline from the lab bench to the synchrotron beamlines, helping users to optimise and prepare their samples for structural studies. As well as accessing advanced equipment and expertise, users can get help from the facility team to analyse data. However, the team frequently faces the problem that the data can only be analysed using the software connected to the equipment and the facility.<\/p>\n\n\n\n<p>\u201cThat means our users would need to be in the facility to analyse their data. This has been a hassle before, but during the 2020 lockdown, it made the work almost impossible,\u201d said Mar\u00eda Garc\u00eda Alai, Team Leader and Head of the SPC Facility. \u201cWorking from home mobilised us to build the eSPC analysis platform, and solve this problem for good. You could see this as a positive aspect of the lockdown.\u201d<\/p>\n\n\n\n<p>The platform enables users to upload and analyse raw data from several experimental techniques: steady-state fluorescence spectroscopy, microscale thermophoresis, and differential scanning fluorimetry experiments. It consists of three modules. <em>FoldAffinity<\/em> quantifies how an interaction between two molecules can further stabilise them. <em>MoltenProt<\/em> helps to estimate how durable a protein is in different conditions. <em>ThermoAffinity<\/em> identifies how strongly two molecules, e.g. a protein and a drug, bind to each other.<\/p>\n\n\n\n<p>\u201cThe platform was foreseen as an educational tool,\u201d explained Garc\u00eda Alai. \u201cWe have implemented different biophysical theoretical models with tutorials that guide non-experts to assess the quality of their collected data, analyse the results, and produce publication-quality figures. We wanted this tool to be accessible to the whole community to push scientific discoveries forward.\u201d<\/p>\n\n\n\n<p>To ensure the eSPC platform meets the needs of the scientific community, the <a href=\"https:\/\/www.embl.org\/groups\/garcia-alai\/\">Garc\u00eda Alai Team<\/a> collected several rounds of feedback from a group of users. Once the tool was made publicly available, Francesca Short, a researcher at Monash University in Australia, was one of the first users to test <em>FoldAffinity<\/em>.<\/p>\n\n\n\n<p>\u201cWe used <em>FoldAffinity<\/em> to study how various molecules bind and influence bacterial proteins that contribute to antimicrobial resistance, a critical public health challenge of this century,\u201d said Short. \u201cWe could only do measurements using methods suitable for small amounts of protein, so it was great for us that <em>FoldAffinity<\/em> allows us to analyse data generated with these methods. We also liked that the output from eSPC was very comprehensive and easy to understand.\u201d<\/p>\n\n\n\n<div class=\"vf-embed vf-embed--16x9 | vf-u-margin__bottom--400\">\n<iframe src=\"https:\/\/www.youtube.com\/embed\/l7RIYVWp5hc\" frameborder=\"0\" controls allowfullscreen><\/iframe><\/div>\n\n  \n<figcaption class=\"vf-figure__caption vf-u-margin__top--200\">eSPC is a free online platform for the analysis of data from diverse molecular biophysics experiments, developed and released by the Sample Preparation and Characterisation (SPC) Facility at EMBL Hamburg and at the Center for Structural Systems Biology (CSSB).<\/figcaption>\n\n\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Looking ahead: the ARISE fellowship to strengthen collaboration<\/strong><\/h2>\n\n\n\n<p>Positive feedback from users encouraged the Garc\u00eda Alai Team to look into expanding the platform in the future. To do this, the team recruited Osvaldo Burastero in this year\u2019s <a href=\"https:\/\/www.embl.org\/about\/info\/arise\/\">ARISE programme<\/a>. ARISE is the first fellowship that trains technology developers and engineers to advance technology and method development in life sciences and to learn how to lead service-providing research infrastructures.<\/p>\n\n\n\n<p>Burastero will spend part of his fellowship at the <a href=\"https:\/\/research.pasteur.fr\/en\/team\/biophysics\/\">Molecular Biophysics facility<\/a> at the Institute Pasteur. The facility already works with the Garc\u00eda Alai Team within <a href=\"https:\/\/www.embl.org\/news\/lab-matters\/spc-mosbri\/\">MOSBRI<\/a>, the pan-European consortium of biophysics facilities. There, he will get hand-on experience in a broad range of new techniques. He will use this knowledge to develop new modules for the platform that cover those techniques.<\/p>\n\n\n\n<p>\u201cFor me, it is a great opportunity to work in the Garc\u00eda Alai Team within the ARISE programme,\u201d said Burastero. \u201cIt lets me learn all the aspects of building scientific software, from the theory behind experiments to managing users. I\u2019m excited to contribute to developing a platform that supports scientists around the world, and I look forward to new developments. I hope we can provide a data analysis platform that other research infrastructure for molecular-scale biophysics will recommend to its users!\u201d<\/p>\n\n\n\n<p>Scientific services are at the heart of <a href=\"https:\/\/www.embl.org\/about\/missions\/\">EMBL\u2019s missions<\/a>, and the SPC Facility\u2019s effort to meet the needs of the scientific community is a great example of that. The SPC Facility supports mainly structural biologists, but eSPC can serve as a bridge to interact with and support other scientific fields, fostering interdisciplinary research.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>EMBL Hamburg\u2019s Sample Preparation and Characterisation (SPC) Facility has released eSPC, an online platform for analysing data from biophysical experiments. The platform enables the scientific community to analyse data from different experiments without the need to travel.<\/p>\n","protected":false},"author":96,"featured_media":43615,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[2,17591],"tags":[11425,18719,65,775,48,11421,53,5744,11419],"embl_taxonomy":[9596,19263,19369],"class_list":["post-43603","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science","category-science-technology","tag-analysis-software","tag-arise","tag-biophysics","tag-cssb","tag-facility","tag-garcia-alai","tag-hamburg","tag-sample-preparation-and-characterisation-facility","tag-services","embl_taxonomy-embl-hamburg","embl_taxonomy-garcia-alai-team","embl_taxonomy-sample-preparation-and-characterisation-facility"],"acf":{"featured":true,"show_featured_image":false,"field_target_display":"","article_intro":"<p>EMBL Hamburg\u2019s Sample Preparation and Characterisation Facility has released an online platform for analysing data from biophysical experiments<\/p>\n","related_links":[{"link_description":" eSPC Platform ","link_url":"https:\/\/spc.embl-hamburg.de\/"},{"link_description":" Sample Preparation and Characterisation Facility ","link_url":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/"},{"link_description":" SPC Facility in Hamburg supports a new pan-European consortium ","link_url":"https:\/\/www.embl.org\/news\/lab-matters\/spc-mosbri\/"},{"link_description":" ARISE Fellowship Programme ","link_url":"https:\/\/www.embl.org\/about\/info\/arise\/"}],"source_article":[{"publication_title":"eSPC: an online data-analysis platform for molecular biophysics","publication_link":{"title":"","url":"https:\/\/journals.iucr.org\/d\/issues\/2021\/10\/00\/qt5002\/index.html","target":"_blank"},"publication_authors":"Burastero O., et al.","publication_source":"Acta Crystallographica Section D","publication_date":"24 September 2021","publication_doi":"10.1107\/S2059798321008998"}],"in_this_article":false,"press_contact":"None","vf_locked":false,"field_article_language":{"value":"english","label":"English"},"article_translations":false,"languages":"","vfwp-news_embl_taxonomy":[9596,19263,19369]},"embl_taxonomy_terms":[{"uuid":"a:3:{i:0;s:36:\"b14d3f13-5670-44fb-8970-e54dfd9c921a\";i:1;s:36:\"89e00fee-87f4-482e-a801-4c3548bb6a58\";i:2;s:36:\"613c4de5-1775-447f-af71-4b07085318e9\";}","parents":[],"name":["EMBL Hamburg"],"slug":"embl-hamburg","description":"Where &gt; All EMBL sites &gt; EMBL Hamburg"},{"uuid":"a:3:{i:0;s:36:\"302cfdf7-365b-462a-be65-82c7b783ebf7\";i:1;s:36:\"2dc39890-6c01-47bf-ac78-d42abdb10079\";i:2;s:36:\"679ec226-70c4-4581-97d1-2a6bcf75b516\";}","parents":[],"name":["Garcia Alai Team"],"slug":"garcia-alai-team","description":"What &gt; Structural Biology (EMBL Hamburg) &gt; Garcia Alai Team"},{"uuid":"a:3:{i:0;s:36:\"302cfdf7-365b-462a-be65-82c7b783ebf7\";i:1;s:36:\"42b86629-bf66-44cd-b9e0-61590f8e8584\";i:2;s:36:\"01cca59b-ea0d-4c7d-b627-4cd295f3f9bc\";}","parents":[],"name":["Sample Preparation and Characterisation Facility"],"slug":"sample-preparation-and-characterisation-facility","description":"What &gt; Services at EMBL Hamburg &gt; Sample Preparation and Characterisation Facility"}],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Biophysics analysis made easy with an online tool | EMBL<\/title>\n<meta name=\"description\" content=\"EMBL Hamburg\u2019s Sample Preparation and Characterisation Facility has released an online platform for analysing data from biophysics experiments\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/science\/biophysics-analysis-made-easy-with-an-online-tool\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biophysics analysis made easy with an online tool | EMBL\" \/>\n<meta property=\"og:description\" content=\"EMBL Hamburg\u2019s Sample Preparation and Characterisation Facility has released an online platform for analysing data from biophysics experiments\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/science\/biophysics-analysis-made-easy-with-an-online-tool\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2021-10-25T08:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-02-16T13:16:40+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2021\/10\/20211001_Newsbanner_eSPC_1000x600px_v2.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1000\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Dorota Badowska\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@d_badowska\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Dorota Badowska\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/biophysics-analysis-made-easy-with-an-online-tool\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/biophysics-analysis-made-easy-with-an-online-tool\/\"},\"author\":{\"name\":\"Dorota Badowska\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/b8ae50efcd7533f0ab2ec368736b1d04\"},\"headline\":\"Biophysics analysis made easy with an online tool\",\"datePublished\":\"2021-10-25T08:00:00+00:00\",\"dateModified\":\"2026-02-16T13:16:40+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/biophysics-analysis-made-easy-with-an-online-tool\/\"},\"wordCount\":761,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/biophysics-analysis-made-easy-with-an-online-tool\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2021\/10\/20211001_Newsbanner_eSPC_1000x600px_v2.jpg\",\"keywords\":[\"analysis software\",\"ARISE\",\"biophysics\",\"cssb\",\"facility\",\"garcia alai\",\"hamburg\",\"sample preparation and characterisation facility\",\"services\"],\"articleSection\":[\"Science\",\"Science &amp; 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