Coat proteins play a key role in the packaging and distribution of proteins within our cells. Studying how these proteins work helps to understand their role in health and disease. Defects in a coat protein complex known as COPII can lead to pathologies related to collagen secretion and lipid metabolism disorders.<\/p>\n\n\n\n
Giulia Zanetti, a group leader at the Institute of Structural and Molecular Biology (ISMB) in London, studies the COPII coat protein complex. Her lab is based at Birkbeck College and uses cryo-electron microscopy (cryo-EM) and cryo-electron tomography to understand coat proteins.<\/p>\n\n\n\n
\u201cWe wanted to use cryo-electron tomography, and at the time, this technique was not so widely used,\u201d Zanetti recalled. \u201cThe EMBL Cryo-EM Service Platform is well established and well known to produce first class data, and that’s why we chose to collect data at EMBL.\u201d<\/p>\n\n\n\n
\u201cThe Cryo-EM Service Platform offers services for cryo-electron microscopy, especially data collection for single particle analysis and cryo-electron tomography,\u201d said Simone Mattei, the EMBL Team Leader in charge of the service platform. \u201cOur team includes members of various backgrounds, including microscopy engineers, application specialists, and image analysts.\u201d<\/p>\n\n\n\n
Zanetti and her team came to EMBL Heidelberg to use cryo-electron tomography to determine the structure of the assembled COPII coat complex. \u201cOur visit was very successful,\u201d said Zanetti. \u201cWe have collected a dataset that allowed us to determine the structure of one of the two layers of the COPII coat on membranes.\u201d<\/p>\n\n\n\n
The visit to Heidelberg not only helped the researchers to advance their specific project, Zanetti added. \u201cOur interaction with the EMBL Cryo-EM Service Platform has definitely improved the way we collect data. We are now using the protocols that we have learned from EMBL here at Birkbeck, which is benefiting not only my group, but also many other colleagues.\u201d<\/p>\n\n\n\n
From this year, the imaging services offered by the Cryo-EM Service Platform will be included in the portfolio of the new EMBL Imaging Centre<\/a>. \u201cThe EMBL Imaging Centre is a new project that builds upon the success of the Cryo-EM Service Platform,\u201d said Mattei. \u201cIt’s a new concept of imaging facility that will host two teams: one for light microscopy and one for electron microscopy. The Imaging Centre will broaden the services offered to our users, including sample preparation for cryo-EM, image processing and analysis.\u201d<\/p>\n\n\n\n Find out more about EMBL\u2019s Scientific and Data Services here<\/a>.<\/p>\n\n\n\n