{"id":32674,"date":"2020-10-08T09:00:33","date_gmt":"2020-10-08T07:00:33","guid":{"rendered":"https:\/\/www.embl.org\/news\/?p=32674"},"modified":"2024-03-22T10:51:50","modified_gmt":"2024-03-22T09:51:50","slug":"beyond-the-lens-microscopy-at-embl-rome","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/lab-matters\/beyond-the-lens-microscopy-at-embl-rome\/","title":{"rendered":"Beyond the lens \u2013 microscopy at EMBL Rome"},"content":{"rendered":"\n

Alvaro Crevenna joined EMBL Rome in March 2019 as Head of the Microscopy Facility<\/a>. He grew up in Mexico where he studied biomedical research and physics as an undergrad, and then followed a research career at several institutes in Europe. During his research he has gained considerable experience in using custom-built microscopy instruments to answer questions in cell biology and biophysics. He has always been keen on helping others to access technologies, so the transition to his current position has been a natural evolution in his career.<\/p>\n\n\n\n

\"Lower<\/a>
New technologies are making it possible to image individual RNA molecules within large tissue samples. Here, the lower part of a mouse brain slice has been processed to render visible all the locations of 10 different RNA species (purple dots), where their single identity will be revealed through subsequent imaging cycles. In cyan are observed single cell nuclei. This technique provides a remarkable level of detail about what gives rise to the many cellular types within a tissue.<\/em> Credit: Alvaro Crevenna\/EMB<\/em>L<\/figcaption><\/figure><\/div>\n\n\n\n

\u201cMicroscopy is an approach to understanding life, a window on the world, with a unique \u2013 perhaps biased \u2013 and sometimes colourful view,\u201d says Alvaro. \u201cMost of my academic career I\u2019ve analysed time-lapse experiments, so science has been a movie. Maybe I just wanted to follow in the footsteps of my grandfather, who was a movie director and made more than 150 films.\u201d<\/p>\n\n\n\n

Building capacity by building a community<\/h2>\n\n\n\n

One of the main missions of the Microscopy Facility at EMBL Rome is to build a local network of microscopy users and collaborators. The facility provides users with training and support, including help with experimental design, image processing, and quantitative image analysis.
To serve a broader community, the facility is currently expanding its services by exporting know-how to partner institutes and engaging in technology transfer.
A collaboration with CrestOptics<\/a> \u2013 a company specialised in microscopy technology \u2013 aims to develop super-resolution microscopy techniques for live cells and fixed tissue samples. EMBL Rome\u2019s Microscopy Facility will serve as a platform for testing and providing insight on innovative imaging solutions, therefore accelerating the time to market.<\/p>\n\n\n\n

Future omics directions<\/h2>\n\n\n\n
\"Mouse<\/a>
Super-resolution microscopy allows for imaging beyond the diffraction limit of light. Here structured illumination microscopy (SIM) is used to monitor how epigenetic changes alter the dynamics of the DNA and its associated proteins in a mouse embryonic fibroblast stained for an epigenetic modifier protein. Credit: Alvaro Crevenna\/EMBL<\/em>
<\/figcaption><\/figure><\/div>\n\n\n\n

The long-term goal is to make the Microscopy Facility at EMBL Rome a centre of excellence for tissue imaging and image analysis. \u201cTo reach that aim, we need not only to foster early adoption of novel technology, but to participate directly in its development,\u201d says Alvaro. For this reason, the facility is committed to developing microscopy-based omics approaches: platforms for multiplex in situ<\/em> tissue profiling using proteomics (to detect tissue-specific proteins), transcriptomics (to identify genes that are expressed by specific cells in a tissue), and connectomics (to study how different areas of the brain are connected to each other). The goal is to carry out these different analyses on the same sample.<\/p>\n\n\n\n

\u201cA multi-plex tissue profiling platform established as a robust service for researchers would be unique in the world,\u201d concludes Alvaro. \u201cI\u2019m really excited about the future discoveries that these techniques will bring forward.\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"

Research facilities play a crucial role in the advancement of science by supporting scientists with specialised expertise and state-of-the-art equipment. The Microscopy Facility at EMBL Rome exemplifies this role by making a wide variety of light microscopy technologies available to its researchers…<\/p>\n","protected":false},"author":92,"featured_media":32678,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3,17591],"tags":[474,227,48,79,514],"embl_taxonomy":[18819,9788],"class_list":["post-32674","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lab-matters","category-science-technology","tag-collaboration","tag-core-facility","tag-facility","tag-microscopy","tag-rome","embl_taxonomy-alvaro-hernan-crevenna-escobar","embl_taxonomy-embl-rome"],"acf":{"featured":true,"show_featured_image":false,"color":"#007B53","link_color":"#fff","article_intro":"

Research facilities play a crucial role in the advancement of science by supporting scientists with specialised expertise and state-of-the-art equipment. The Microscopy Facility at EMBL Rome exemplifies this role by making a wide variety of light microscopy technologies available to its researchers and the external scientific community.<\/p>\n","related_links":[{"link_description":"EMBL Rome Microscopy Facility","link_url":"https:\/\/www.embl.it\/services\/microscopy\/"},{"link_description":"","link_url":""}],"article_sources":false,"in_this_article":false,"youtube_url":"","mp4_url":"","video_caption":"","press_contact":"None","translations":false,"vf_locked":false},"embl_taxonomy_terms":[{"uuid":"a:2:{i:0;s:36:\"4428d1fd-441a-4d6d-a1c5-5dcf5665f213\";i:1;s:36:\"a79c7024-c640-4ed7-8d9f-6905ce6f4703\";}","parents":[],"name":["Alvaro Hernan Crevenna Escobar"],"slug":"alvaro-hernan-crevenna-escobar","description":"Who > Alvaro Hernan Crevenna Escobar"},{"uuid":"a:3:{i:0;s:36:\"b14d3f13-5670-44fb-8970-e54dfd9c921a\";i:1;s:36:\"89e00fee-87f4-482e-a801-4c3548bb6a58\";i:2;s:36:\"741d5d3d-f92f-4eb8-9195-55c96454a36b\";}","parents":[],"name":["EMBL Rome"],"slug":"embl-rome","description":"Where > All EMBL sites > EMBL Rome"}],"yoast_head":"\nBeyond the lens \u2013 microscopy at EMBL Rome | EMBL<\/title>\n<meta name=\"description\" content=\"Research facilities play a crucial role in the advancement of science by supporting scientists with specialised expertise and state-of-the-art equipment. 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