{"id":2437,"date":"2014-11-03T12:49:04","date_gmt":"2014-11-03T11:49:04","guid":{"rendered":"http:\/\/news.embl.de\/?p=2437"},"modified":"2025-02-19T13:37:29","modified_gmt":"2025-02-19T12:37:29","slug":"1411_pepc","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/","title":{"rendered":"PEPC recipe for success"},"content":{"rendered":"\n<p>To help filter gold out of the garbage, the ninth Protein Expression, Purification and Characterization EMBO practical course was held at EMBL Hamburg in September. The course focuses on state-of-the-art techniques in sample preparation for structural biology experiments, which require large amounts of pure, high-quality biological samples.<\/p>\n\n\n\n<p>The hands-on course attracted 20 students from 15 countries for an intensive eight-day programme of lectures and practical sessions, with a blend of basic training in expression and purification techniques, and the latest developments in the use of subsequent samples. A highlight was the presentation by Petra Fromme of Arizona State University on new sample delivery techniques for free electron lasers, including recent results from a study of different light\/dark stages of photosystem II, the first link in the chain of photosynthesis. Another high point was when Yiorgo Skiniotis of the University of Michigan showed how improvements in sample preparation and detector technologies enabled the study of the catalytic cycles of a large multi-component enzyme using electron microscopy.<\/p>\n\n\n\n<p>Students were encouraged to provide their own samples and could use facilities at EMBL Hamburg, EMBL Grenoble and the Oxford Protein Production Facility UK (OPPF), thanks to the close collaboration between the three facilities for the course organisation. OPPF cloned, expressed and purified samples from 10 participants, while the Berger group in Grenoble tested expression in baculovirus, providing several participants with promising enough results to continue at home or in collaboration with the facilities. The sample preparation and characterisation facility at EMBL Hamburg provided thermal shift assays, and several samples were crystallised for the first time during the course.<\/p>\n\n\n\n<p>Since the attendees\u2019 samples were processed before and during the course, they could discuss their ongoing projects with tutors, prompting open and detailed discussions about how to apply what they learned, and when to deviate from the well-trodden path. The opportunity to discuss strategies with world-leading experts was greatly appreciated: \u201cEven though the crystallisation experiments did not work, it was really good to be able to discuss and understand why,\u201d said an attendee from the University of Graz. \u201cWe already have results from the protein samples,\u201d added another from the University of M\u00fcnster. \u201cHaving discussed the results with the experts, I can return to my lab with new ideas about how to continue with my project.\u201d<\/p>\n\n\n\n<p>For details of the next course, to be held in September 2016, please contact <a title=\"Rob Meijers\" href=\"http:\/\/www.embl-hamburg.de\/research\/unit\/meijers\/members\/?s_personId=CP-60006566\" target=\"_blank\" rel=\"noopener noreferrer\">Rob Meijers<\/a> \u2013 scientific co-organiser and EMBL Hamburg group leader \u2013 or <a title=\"EMBO Events e-newsletter\" href=\"http:\/\/www.embo.org\/news\/e-news\" target=\"_blank\" rel=\"noopener noreferrer\">subscribe to the EMBO events e-newsletter<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>How to make good quality samples for good quality structural biology experiments.<\/p>\n","protected":false},"author":18,"featured_media":2440,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[4,17591],"tags":[29,53,35,27],"embl_taxonomy":[],"class_list":["post-2437","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-events","category-science-technology","tag-crystallography","tag-hamburg","tag-structural-biology","tag-training"],"acf":{"article_intro":"<p>Sample quality sets the stage for the outcome of any experiment: you can\u2019t make a silk purse out of a sow\u2019s ear, as the saying goes \u2013 or more specifically, if you put garbage into your fancy equipment you will get garbage out.<\/p>\n","related_links":[{"link_description":"Upcoming courses and conference at EMBL Hamburg","link_url":"http:\/\/www.embl-hamburg.de\/training\/events\/index.php"},{"link_description":"Sample Preparation and Characterization (SPC) facility, EMBL Hamburg","link_url":"http:\/\/www.embl-hamburg.de\/services\/spc\/index.html"},{"link_description":"Structural biology services, EMBL Grenoble ","link_url":"http:\/\/www.embl.fr\/services\/index.html"},{"link_description":"EMBO practical course: PEPC9","link_url":"http:\/\/events.embo.org\/14-pepc\/"}],"article_sources":false,"vf_locked":false,"featured":false,"color":"#007B53"},"embl_taxonomy_terms":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>PEPC recipe for success | EMBL<\/title>\n<meta name=\"description\" content=\"How to make good quality samples for good quality structural biology experiments.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PEPC recipe for success | EMBL\" \/>\n<meta property=\"og:description\" content=\"How to make good quality samples for good quality structural biology experiments.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2014-11-03T11:49:04+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-02-19T12:37:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"620\" \/>\n\t<meta property=\"og:image:height\" content=\"425\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Rosemary Wilson\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@rawilson80\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Rosemary Wilson\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/\"},\"author\":{\"name\":\"Rosemary Wilson\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/bb5e57a6c6c5c3b33a6a40b2d4c96e40\"},\"headline\":\"PEPC recipe for success\",\"datePublished\":\"2014-11-03T11:49:04+00:00\",\"dateModified\":\"2025-02-19T12:37:29+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/\"},\"wordCount\":412,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg\",\"keywords\":[\"crystallography\",\"hamburg\",\"structural biology\",\"training\"],\"articleSection\":[\"Events\",\"Science &amp; Technology\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/\",\"url\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/\",\"name\":\"PEPC recipe for success | EMBL\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg\",\"datePublished\":\"2014-11-03T11:49:04+00:00\",\"dateModified\":\"2025-02-19T12:37:29+00:00\",\"description\":\"How to make good quality samples for good quality structural biology experiments.\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage\",\"url\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg\",\"contentUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg\",\"width\":620,\"height\":425,\"caption\":\"Twenty students from 15 countries joined leading experts in Hamburg for the PEPC9 course, 8\u201316 September 2014. PHOTO: Stephane Boivin\"},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.embl.org\/news\/#website\",\"url\":\"https:\/\/www.embl.org\/news\/\",\"name\":\"European Molecular Biology Laboratory News\",\"description\":\"News from the European Molecular Biology Laboratory\",\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"alternateName\":\"EMBL News\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.embl.org\/news\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.embl.org\/news\/#organization\",\"name\":\"European Molecular Biology Laboratory\",\"alternateName\":\"EMBL\",\"url\":\"https:\/\/www.embl.org\/news\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png\",\"contentUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png\",\"width\":300,\"height\":144,\"caption\":\"European Molecular Biology Laboratory\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/embl.org\/\",\"https:\/\/x.com\/embl\",\"https:\/\/www.instagram.com\/embl_org\/\",\"https:\/\/www.linkedin.com\/company\/15813\/\",\"https:\/\/www.youtube.com\/user\/emblmedia\/\"]},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/bb5e57a6c6c5c3b33a6a40b2d4c96e40\",\"name\":\"Rosemary Wilson\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/8358153869957da911fe3f14a8657c5b102e1b99f4f4d385ce4d402432a7acc8?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/8358153869957da911fe3f14a8657c5b102e1b99f4f4d385ce4d402432a7acc8?s=96&d=mm&r=g\",\"caption\":\"Rosemary Wilson\"},\"description\":\"Rosemary Wilson is training and outreach officer at EMBL Hamburg. A passionate science communicator, she enjoys sharing her enthusiasm for the fascinating projects happening around her on the DESY campus.\",\"sameAs\":[\"https:\/\/x.com\/rawilson80\"],\"url\":\"https:\/\/www.embl.org\/news\/author\/rosemary\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"PEPC recipe for success | EMBL","description":"How to make good quality samples for good quality structural biology experiments.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/","og_locale":"en_US","og_type":"article","og_title":"PEPC recipe for success | EMBL","og_description":"How to make good quality samples for good quality structural biology experiments.","og_url":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/","og_site_name":"EMBL","article_publisher":"https:\/\/www.facebook.com\/embl.org\/","article_published_time":"2014-11-03T11:49:04+00:00","article_modified_time":"2025-02-19T12:37:29+00:00","og_image":[{"width":620,"height":425,"url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg","type":"image\/jpeg"}],"author":"Rosemary Wilson","twitter_card":"summary_large_image","twitter_creator":"@rawilson80","twitter_site":"@embl","twitter_misc":{"Written by":"Rosemary Wilson","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"NewsArticle","@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#article","isPartOf":{"@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/"},"author":{"name":"Rosemary Wilson","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/bb5e57a6c6c5c3b33a6a40b2d4c96e40"},"headline":"PEPC recipe for success","datePublished":"2014-11-03T11:49:04+00:00","dateModified":"2025-02-19T12:37:29+00:00","mainEntityOfPage":{"@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/"},"wordCount":412,"publisher":{"@id":"https:\/\/www.embl.org\/news\/#organization"},"image":{"@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage"},"thumbnailUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg","keywords":["crystallography","hamburg","structural biology","training"],"articleSection":["Events","Science &amp; Technology"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/","url":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/","name":"PEPC recipe for success | EMBL","isPartOf":{"@id":"https:\/\/www.embl.org\/news\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage"},"image":{"@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage"},"thumbnailUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg","datePublished":"2014-11-03T11:49:04+00:00","dateModified":"2025-02-19T12:37:29+00:00","description":"How to make good quality samples for good quality structural biology experiments.","inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.embl.org\/news\/events\/1411_pepc\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/events\/1411_pepc\/#primaryimage","url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg","contentUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg","width":620,"height":425,"caption":"Twenty students from 15 countries joined leading experts in Hamburg for the PEPC9 course, 8\u201316 September 2014. PHOTO: Stephane Boivin"},{"@type":"WebSite","@id":"https:\/\/www.embl.org\/news\/#website","url":"https:\/\/www.embl.org\/news\/","name":"European Molecular Biology Laboratory News","description":"News from the European Molecular Biology Laboratory","publisher":{"@id":"https:\/\/www.embl.org\/news\/#organization"},"alternateName":"EMBL News","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.embl.org\/news\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.embl.org\/news\/#organization","name":"European Molecular Biology Laboratory","alternateName":"EMBL","url":"https:\/\/www.embl.org\/news\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/","url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png","contentUrl":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2025\/09\/EMBL_logo_colour-1-300x144-1.png","width":300,"height":144,"caption":"European Molecular Biology Laboratory"},"image":{"@id":"https:\/\/www.embl.org\/news\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/embl.org\/","https:\/\/x.com\/embl","https:\/\/www.instagram.com\/embl_org\/","https:\/\/www.linkedin.com\/company\/15813\/","https:\/\/www.youtube.com\/user\/emblmedia\/"]},{"@type":"Person","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/bb5e57a6c6c5c3b33a6a40b2d4c96e40","name":"Rosemary Wilson","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.embl.org\/news\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/8358153869957da911fe3f14a8657c5b102e1b99f4f4d385ce4d402432a7acc8?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8358153869957da911fe3f14a8657c5b102e1b99f4f4d385ce4d402432a7acc8?s=96&d=mm&r=g","caption":"Rosemary Wilson"},"description":"Rosemary Wilson is training and outreach officer at EMBL Hamburg. A passionate science communicator, she enjoys sharing her enthusiasm for the fascinating projects happening around her on the DESY campus.","sameAs":["https:\/\/x.com\/rawilson80"],"url":"https:\/\/www.embl.org\/news\/author\/rosemary\/"}]}},"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"fimg_url":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg","featured_image_src":"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2014\/11\/Groupe_photo-extended.jpg","_links":{"self":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/2437","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/users\/18"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/comments?post=2437"}],"version-history":[{"count":6,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/2437\/revisions"}],"predecessor-version":[{"id":19549,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/posts\/2437\/revisions\/19549"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/media\/2440"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/media?parent=2437"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/categories?post=2437"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/tags?post=2437"},{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/news\/wp-json\/wp\/v2\/embl_taxonomy?post=2437"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}