{"id":15434,"date":"2019-03-11T11:00:44","date_gmt":"2019-03-11T10:00:44","guid":{"rendered":"https:\/\/news.embl.de\/?p=15434"},"modified":"2024-03-22T11:19:27","modified_gmt":"2024-03-22T10:19:27","slug":"atp-affects-proteome-wide-solubility","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/science\/atp-affects-proteome-wide-solubility\/","title":{"rendered":"ATP affects proteome-wide solubility"},"content":{"rendered":"\n<p>A collaboration between EMBL Heidelberg&#8217;s&nbsp;<a href=\"https:\/\/www.embl.de\/research\/units\/genome_biology\/savitski\/index.html\">Savitski team<\/a>&nbsp;and GSK\u2019s Cellzome has developed a new technology to study how the concentration of a molecule affects the solubility of individual proteins on a proteome-wide scale. In their paper, published in <em>Nature Communications,&nbsp;<\/em>Sindhuja Sridharan, Nils Kurzawa and colleagues used Solubility Proteome Profiling (SPP) to show that adenosine triphosphate (ATP) affects the solubility of at least 25% of the <a href=\"https:\/\/news.embl.de\/science\/protein-stability-solubility-cell-cycle\/?_ga=2.169980387.251723274.1551452573-308861870.1544644917\">solubility-transitioning<\/a> proteins in mammalian cells.<\/p>\n\n\n\n<p>ATP performs diverse biological roles, most famously as the cell\u2019s primary energy source. However, the lack of system-wide studies in realistic conditions limits our knowledge of many of its other functions. To help address this, the team has provided a quantitative, proteome-wide insight into how ATP affects protein structure and solubility across the range of concentrations seen in cells.<\/p>\n\n\n\n<p>They discovered that ATP preferentially affects the solubility of proteins that are positively charged, naturally disordered, nucleic acid-binding and part of membraneless organelles. The susceptibility of a protein to solubilise depends on its localisation to the different types of these organelles. These findings provide the first clues to how common metabolites, like ATP, affect cellular function and organisation.<\/p>\n\n\n\n<p>SPP uses mass spectrometry to determine the effect of a molecule\u2019s concentration on the solubility of individual proteins on a proteome-wide scale. In future studies, SPP could be used to measure how drugs affect protein solubility. This would allow scientists to assess the effectiveness of drugs that aim to dissolve protein aggregates \u2013 clumps of mis-folded proteins that are associated with diseases such as Alzheimer\u2019s or Parkinson\u2019s.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Scientists develop technology to measure how ATP concentration affects protein solubility in cells<\/p>\n","protected":false},"author":67,"featured_media":15435,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[2,17591],"tags":[64,591,537,981,496,155,43,248,45,539,434],"embl_taxonomy":[],"class_list":["post-15434","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science","category-science-technology","tag-cell-biology","tag-cellzome","tag-dna","tag-drug","tag-genome-biology","tag-gsk","tag-heidelberg","tag-mass-spectrometry","tag-proteomics","tag-research-highlight","tag-savitski"],"acf":{"article_intro":"<p><span lang=\"EN-GB\">Scientists develop technology to measure how ATP concentration affects protein solubility in cells<\/span><\/p>\n","related_links":[{"link_description":"Research in the Savitski team","link_url":"https:\/\/www.embl.de\/research\/units\/genome_biology\/savitski\/index.html"},{"link_description":"Protein stability and solubility during cell cycle","link_url":"https:\/\/news.embl.de\/science\/protein-stability-solubility-cell-cycle\/?_ga=2.169980387.251723274.1551452573-308861870.1544644917"},{"link_description":"The EMBL-GSK collaboration","link_url":"https:\/\/www.embl.de\/aboutus\/communication_outreach\/media_relations\/2017\/170216-GSK-collaboration\/"}],"article_sources":[{"source_description":"<p>Sridharan S, Kurzawa N, <em>et al<\/em>. Proteome-wide solubility and thermal stability profiling reveals distinct regulatory roles for ATP. <em>Nature Communications<\/em>. Published online 11 March 2019.<\/p>\n","source_link_url":"https:\/\/doi.org\/10.1038\/s41467-019-09107-y"}],"vf_locked":false,"featured":false,"color":"#007B53"},"embl_taxonomy_terms":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>ATP affects proteome-wide solubility | EMBL<\/title>\n<meta name=\"description\" content=\"Scientists develop new technology, Solubility Proteome Profiling, to measure how ATP concentration affects protein solubility in cells\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.embl.org\/news\/science\/atp-affects-proteome-wide-solubility\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ATP affects proteome-wide solubility | EMBL\" \/>\n<meta property=\"og:description\" content=\"Scientists develop new technology, Solubility Proteome Profiling, to measure how ATP concentration affects protein solubility in cells\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.embl.org\/news\/science\/atp-affects-proteome-wide-solubility\/\" \/>\n<meta property=\"og:site_name\" content=\"EMBL\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/embl.org\/\" \/>\n<meta property=\"article:published_time\" content=\"2019-03-11T10:00:44+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-03-22T10:19:27+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2019\/03\/1903_savitski_naturecommsvisual-ib.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"620\" \/>\n\t<meta property=\"og:image:height\" content=\"425\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Josh Tapley\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@embl\" \/>\n<meta name=\"twitter:site\" content=\"@embl\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Josh Tapley\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\/\/www.embl.org\/news\/science\/atp-affects-proteome-wide-solubility\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/atp-affects-proteome-wide-solubility\/\"},\"author\":{\"name\":\"Josh Tapley\",\"@id\":\"https:\/\/www.embl.org\/news\/#\/schema\/person\/d242d2d21f1166a7e8e67e3e28fd5479\"},\"headline\":\"ATP affects proteome-wide solubility\",\"datePublished\":\"2019-03-11T10:00:44+00:00\",\"dateModified\":\"2024-03-22T10:19:27+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/atp-affects-proteome-wide-solubility\/\"},\"wordCount\":266,\"publisher\":{\"@id\":\"https:\/\/www.embl.org\/news\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.embl.org\/news\/science\/atp-affects-proteome-wide-solubility\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.embl.org\/news\/wp-content\/uploads\/2019\/03\/1903_savitski_naturecommsvisual-ib.jpg\",\"keywords\":[\"cell biology\",\"cellzome\",\"dna\",\"drug\",\"genome biology\",\"gsk\",\"heidelberg\",\"mass spectrometry\",\"proteomics\",\"research highlight\",\"savitski\"],\"articleSection\":[\"Science\",\"Science &amp; 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