{"id":14269,"date":"2018-09-13T17:00:29","date_gmt":"2018-09-13T15:00:29","guid":{"rendered":"https:\/\/news.embl.de\/?p=14269"},"modified":"2024-03-22T11:05:04","modified_gmt":"2024-03-22T10:05:04","slug":"x-rays-shape-pdzk1","status":"publish","type":"post","link":"https:\/\/www.embl.org\/news\/science\/x-rays-shape-pdzk1\/","title":{"rendered":"X-rays reveal surprising shape of scaffolding protein"},"content":{"rendered":"\n<p>While bioinformatics tools had suggested that the four domains of PDZK1 would behave like beads on a string, moving around in a highly flexible manner, X-ray experiments now show that PDZK1 actually has a relatively well-defined L-shaped conformation with only moderate flexibility.<\/p>\n\n\n\n<p>This discovery was made by a research team led by EMBL group leaders Dmitri Svergun and Christian L\u00f6w \u2013 who is also a group leader at the Centre for Structural Systems Biology (CSSB) in Hamburg, Germany. The journal <em>Structure<\/em> published their results on 13 September 2018.<\/p>\n\n\n\n<p>Scaffolding proteins like PDZK1 mediate interactions between proteins on the membranes of human cells. L\u00f6w, Svergun and colleagues performed small-angle X-ray scattering (SAXS) studies at DESY\u2019s X-ray light source PETRA III to obtain the new information on the shapes and arrangement of PDKZK1\u2019s domains, as well as on their interactions.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>X-ray experiments show that scaffolding protein PDZK1 has a L-shaped conformation<\/p>\n","protected":false},"author":58,"featured_media":14270,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[2,17591],"tags":[53,461,539,251,250,708],"embl_taxonomy":[],"class_list":["post-14269","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science","category-science-technology","tag-hamburg","tag-low","tag-research-highlight","tag-small-angle-x-ray-scattering-saxs","tag-svergun","tag-x-ray"],"acf":{"article_intro":"<p>The shape of PDZK1, an important scaffolding protein in cells, is full of surprises<\/p>\n","related_links":[{"link_description":"","link_url":""},{"link_description":"","link_url":""},{"link_description":"","link_url":""}],"article_sources":[{"source_description":"<p>Probing the Architecture of a Multi-PDZ Domain Protein: Structure of PDZK1 in Solution; Nelly R. Hajizadeh, Joanna Pieprzyk, Petr Skopintsev, Ali Flayhan, Dmitri I. Svergun, Christian L\u00f6w; <em>Structure<\/em>, 2018; DOI: 10.1016\/j.str.2018.07.016<\/p>\n","source_link_url":"http:\/\/www.cell.com\/structure\/fulltext\/S0969-2126(18)30262-4"}],"vf_locked":false,"featured":false,"color":"#007B53","show_featured_image":false,"field_target_display":"embl","field_article_language":{"value":"english","label":"English"},"source_article":false,"in_this_article":false,"press_contact":"None","article_translations":false,"languages":""},"embl_taxonomy_terms":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>X-rays reveal surprising shape of scaffolding protein | EMBL<\/title>\n<meta name=\"description\" content=\"X-ray experiments reveal that scaffolding protein PDZK1 has a well-defined L-shaped conformation with only moderate flexibility.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link 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