{"id":336,"date":"2025-07-22T12:43:28","date_gmt":"2025-07-22T12:43:28","guid":{"rendered":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/?page_id=336"},"modified":"2025-07-30T07:08:03","modified_gmt":"2025-07-30T07:08:03","slug":"sample-preparation-laboratory","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/sample-preparation-laboratory\/","title":{"rendered":"Sample Preparation Laboratory"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The sample preparation laboratory is available for people who have been awarded X-ray imaging beamtime. The laboratory is a small laboratory close to the beamline, where final sample preparation can be done as well as adjustments during the beamtime to optimise samples based on experience gained during data collection.<\/p>\n\n\n\n<p>Before entering the sample preparation laboratory, it is necessary to watch a short introduction and instruction presentation (online), with a short quiz in the end.&nbsp;<\/p>\n\n\n\n<p>Further, we ask to provide the state of wet-lab knowledge before you arrive on site to provide a short lab introduction on site, if necessary.&nbsp;<\/p>\n\n\n\n<p>See also: <\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/before-your-beamtime\/#vf-tabs__section-sample-preparation\" data-type=\"link\" data-id=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/before-your-beamtime\/#vf-tabs__section-sample_preparation\">Prepare your samples<\/a><\/li>\n\n\n\n<li>Lab safety introduction (coming soon)<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1156-1024x768.jpeg\" alt=\"\" class=\"wp-image-615\" srcset=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1156-1024x768.jpeg 1024w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1156-300x225.jpeg 300w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1156-768x576.jpeg 768w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1156-1536x1152.jpeg 1536w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1156-2048x1536.jpeg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Sample preparation laboratory close to P14.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-available-equipment\" data-vf-js-location-nearest-activation-target=\"\">Available equipment<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-available-chemicals\" data-vf-js-location-nearest-activation-target=\"\">Available chemicals<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-how-to-clean-the-lab\" data-vf-js-location-nearest-activation-target=\"\">How to clean the lab<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-available-equipment\"><h2>Available equipment<\/h2>\n<h3 class=\"wp-block-heading\">General sample mounting equipment<\/h3>\n\n\n\n<p>We have a fully equipped laboratory with all the tools required for preparing a HiTT sample of regular specimens. For short beamtimes (less than 48h p.a.), we can provide Pucks, Goniometer bases, and goniometer caps for use on-site.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1118-768x1024.jpeg\" alt=\"\" class=\"wp-image-350\" srcset=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1118-768x1024.jpeg 768w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1118-225x300.jpeg 225w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1118-1152x1536.jpeg 1152w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1118-1536x2048.jpeg 1536w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1118-scaled.jpeg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Centrifuge<\/h3>\n\n\n\n<p><strong><em>Eppendorf miniSpin Centrifuge<\/em><\/strong><\/p>\n\n\n\n<p>The laboratory is equipped with an Eppendorf Mini centrifuge, which can be used for sample preparation and mounting.<\/p>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1125-768x1024.jpeg\" alt=\"\" class=\"wp-image-355\" srcset=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1125-768x1024.jpeg 768w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1125-225x300.jpeg 225w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1125-1152x1536.jpeg 1152w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1125-1536x2048.jpeg 1536w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1125-scaled.jpeg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Weight scales<\/h3>\n\n\n\n<p><strong><em>OHAUS Discovery Semi-Micro and Analytical &#8211; DV215CD<\/em><\/strong><\/p>\n\n\n\n<p>Two different weight scales are available on-site in the sample preparation laboratory. One is a general lab scale, and the other is a high precision shielded weight scale under a chemical fume hood.<\/p>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1119-768x1024.jpeg\" alt=\"\" class=\"wp-image-351\" srcset=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1119-768x1024.jpeg 768w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1119-225x300.jpeg 225w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1119-1152x1536.jpeg 1152w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1119-1536x2048.jpeg 1536w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1119-scaled.jpeg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Magnetic heat stirrer<\/h3>\n\n\n\n<p><strong><em>Phoenix Instrument &#8211; RSM-10HP<\/em><\/strong><\/p>\n\n\n\n<p>A basic magnetic heat stirrer is available in the lab for sample preparation and mounting.<\/p>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1121-768x1024.jpeg\" alt=\"\" class=\"wp-image-352\" srcset=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1121-768x1024.jpeg 768w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1121-225x300.jpeg 225w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1121-1152x1536.jpeg 1152w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1121-1536x2048.jpeg 1536w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1121-scaled.jpeg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Stereo Microscope<\/h3>\n\n\n\n<p><em><strong>Leica Microsystems M80 Stereo Microscope<\/strong><\/em><\/p>\n\n\n\n<p>The on-site stereo microscope can be used to prepare small samples.<\/p>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1124-768x1024.jpeg\" alt=\"\" class=\"wp-image-354\" srcset=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1124-768x1024.jpeg 768w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1124-225x300.jpeg 225w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1124-1152x1536.jpeg 1152w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1124-1536x2048.jpeg 1536w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1124-scaled.jpeg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Vortex mixer <\/h3>\n\n\n\n<p><strong><em>Stuart SA8 Vortex Mixer<\/em><\/strong><\/p>\n\n\n\n<p>The Vortex mixer enables rapid mixing of samples contained in tubes.<\/p>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1126-1024x768.jpeg\" alt=\"\" class=\"wp-image-356\" srcset=\"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1126-1024x768.jpeg 1024w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1126-300x225.jpeg 300w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1126-768x576.jpeg 768w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1126-1536x1152.jpeg 1536w, https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-content\/uploads\/2025\/07\/IMG_1126-2048x1536.jpeg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Label printer<\/h3>\n\n\n\n<p><strong><em>Brother QL-820NWBc<\/em><\/strong><\/p>\n\n\n\n<p>The lab has a pre-set label printer connected to the beamline database. This enables a fast and streamlined preparation in the laboratory. <\/p>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-available-chemicals\"><h2>Available chemicals<\/h2>\n<p>General liquids and solid chemicals used for our established sample preparation are available in the sample preparation laboratory. A short list can be found below:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Ethanol (different concentrations)<\/li>\n\n\n\n<li>Paraformaldehyde 5% solution (PFA)<\/li>\n\n\n\n<li>Phosphate Solution Buffer 10x (PBS)<\/li>\n\n\n\n<li>Acetone<\/li>\n\n\n\n<li>Distilled water<\/li>\n\n\n\n<li>MilliQ water<\/li>\n<\/ul>\n\n\n\n<p>Please note that, the X-ray imaging group can only help with the sample preparation and mounting needed for the acquisition at P14.<\/p>\n\n\n\n<p>We do not provide embedding possibilities on-site.<\/p>\n\n\n\n<p>If you need more or specific chemicals, please review the contact form below to contact the Duke Team.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-how-to-clean-the-lab\"><h2>How to clean the lab<\/h2>\n<p>After the usage of the sample preparation laboratory, it is very important to follow the cleaning instructions, mentioned in the online training as well as below. Please carefully follow the instructions to enable a tidy and clean laboratory and equipment for the next user.<\/p>\n\n\n\n<div class=\"vf-embed vf-embed--16x9 | vf-u-margin__bottom--400\"\n>\n<iframe src=\"https:\/\/www.youtube.com\/embed\/DS1VEJrYTxk\" frameborder=\"0\" controls allowfullscreen><\/iframe><\/div>\n\n  \n<figcaption class=\"vf-figure__caption vf-u-margin__top--200\">Cleaning of the sample preparation laboratory &#8211; HiTT imaging at P14<\/figcaption>\n\n\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-content--grey-box\">\n\n\n\n<h3 class=\"wp-block-heading\">Get in touch with the lab manager&nbsp;<\/h3>\n\n\n<div class=\"vf-content-hub-html\" data-cache=\"6e1fdb77\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-contains%5Bfield_person_full_name%5D=hessel&amp;filter-fields-empty=field_person_visible_internally&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bteam%5D=1&amp;limit=1&amp;pattern=vf-profile-inline -->\n                \n                      <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"190349\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/assets.emblstatic.net\/vf\/v2.4.14\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" alt=\"image of Lukas Hessel\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/lukas-hessel\" class=\"vf-profile__link\">Lukas Hessel<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-profile__job-title\">\n          Predoctoral Fellow\n        <\/p>\n      \n      \n      \n      \n      \n      \n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/190349\/190349\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n\n\n\n<div style=\"height:0px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Please contact the sample preparation laboratory manager if you have any questions about the equipment and chemicals in the lab and sample preparation.<\/p>\n\n\n\n<\/div>\n\n\n\n<div style=\"height:32px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<style>\n.vf-content--grey-box {\n  background-color: #fafafa;\n  padding: 1.5rem;\n  border-radius: 6px;\n  margin-top: 1rem;\n  margin-bottom: 2rem;\n}\n<\/style>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":6,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-336","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/pages\/336","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/comments?post=336"}],"version-history":[{"count":52,"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/pages\/336\/revisions"}],"predecessor-version":[{"id":1503,"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/pages\/336\/revisions\/1503"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/media?parent=336"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/x-ray-imaging\/wp-json\/wp\/v2\/embl_taxonomy?post=336"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}