{"id":65,"date":"2021-04-02T22:36:15","date_gmt":"2021-04-02T22:36:15","guid":{"rendered":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/?page_id=65"},"modified":"2024-09-27T12:41:15","modified_gmt":"2024-09-27T12:41:15","slug":"experimental-possibilities","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/experimental-possibilities\/","title":{"rendered":"Experimental possibilities"},"content":{"rendered":"\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/WhatsApp-Image-2024-08-02-at-12.23.33-PM5-4-1024x766.jpeg\" alt=\"\" class=\"wp-image-10373\" width=\"256\" height=\"192\" srcset=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/WhatsApp-Image-2024-08-02-at-12.23.33-PM5-4-1024x766.jpeg 1024w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/WhatsApp-Image-2024-08-02-at-12.23.33-PM5-4-300x224.jpeg 300w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/WhatsApp-Image-2024-08-02-at-12.23.33-PM5-4-768x575.jpeg 768w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/WhatsApp-Image-2024-08-02-at-12.23.33-PM5-4-1536x1149.jpeg 1536w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/WhatsApp-Image-2024-08-02-at-12.23.33-PM5-4.jpeg 1600w\" sizes=\"auto, (max-width: 256px) 100vw, 256px\" \/><figcaption class=\"vf-figure__caption\">SAXS scattering image of solution sample.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-entrance-1024x683.jpg\" alt=\"\" class=\"wp-image-10265\" srcset=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-entrance-1024x683.jpg 1024w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-entrance-300x200.jpg 300w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-entrance-768x512.jpg 768w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-entrance-1536x1025.jpg 1536w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-entrance.jpg 1686w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Entrance to P12 experimental hall<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-detector-1024x683.jpg\" alt=\"\" class=\"wp-image-10283\" srcset=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-detector-1024x683.jpg 1024w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-detector-300x200.jpg 300w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-detector-768x512.jpg 768w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-detector-1536x1024.jpg 1536w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/08\/p12-detector.jpg 1687w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Dector at P12.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/05\/20240209_1503571-1024x576.jpg\" alt=\"\" class=\"wp-image-9419\" srcset=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/05\/20240209_1503571-1024x576.jpg 1024w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/05\/20240209_1503571-300x169.jpg 300w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/05\/20240209_1503571-768x432.jpg 768w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/05\/20240209_1503571-1536x864.jpg 1536w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/05\/20240209_1503571-2048x1152.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Your SAXS guides at P12.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/09\/2dimage-1024x768.png\" alt=\"\" class=\"wp-image-11763\" srcset=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/09\/2dimage-1024x768.png 1024w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/09\/2dimage-300x225.png 300w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/09\/2dimage-768x576.png 768w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/09\/2dimage-1536x1152.png 1536w, https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-content\/uploads\/2024\/09\/2dimage.png 1920w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Scattering pattern of lipo-based nanoparticles.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p><strong>Experimental Possibilities (SAXS)<\/strong> <strong>with Small Angle X-ray Scattering<\/strong><\/p>\n\n\n\n<p>The SAXS beamline at EMBL offers a wide range of experimental capabilities, designed to meet the diverse needs of the scientific community. <\/p>\n\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Key Experimental Techniques:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/home\/sample-environments\/automated-saxs\/\"><strong>High-Throughput SAXS<\/strong>:<\/a> Achieve rapid data collection using an automated liquid handling system. With an in-vacuum setup, sample volumes as small as 30 \u00b5l can be used per measurement, and exposure times range from 1-4 seconds depending on experimental conditions. Note, the whole measurement cycle with cleaning and drying of the capillary takes ~ 90 seconds. <\/li>\n\n\n\n<li><strong><a href=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/home\/sample-environments\/automated-saxs\/#sec-saxs\">Chromatography-Coupled SAXS<\/a>:<\/strong> Perform size-exclusion chromatography in-line with SAXS measurements (SEC-SAXS). This approach allows the analysis of heterogeneous samples and ensures sample monodispersity before SAXS data collection. Similarly, we now offer the coupling of <a href=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/home\/sample-environments\/automated-saxs\/#af4-saxs\">Asymmetric Flow Field-Flow Fractionation<\/a> (AF4) to the SAXS capillary, expanding analysis opportunities to larger length scales and across a wider variety of sample types.<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Anomalous SAXS (ASAXS)<\/strong>: Study element-specific scattering using ASAXS, which enhances contrast by tuning the X-ray energy near the absorption edge of selected elements. This is ideal for examining complex samples containing metals or distinguishing between components in a mixture. ASAXS can also be combined with various sample environments, allowing for controlled temperature or time-resolved studies.<\/li>\n\n\n\n<li><strong>Wide-Angle X-ray Scattering (WAXS)<\/strong>: The WAXS setup extends the measurement range to s\u2248 25 1\/nm for samples requiring higher resolution structural details.<\/li>\n\n\n\n<li><a href=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/home\/sample-environments\/time-resolved-saxs-tr-saxs\/\"><strong>Time-Resolved SAXS<\/strong>:<\/a> Our setup allows for sub-millisecond time resolution, ideal for capturing fast structural changes in dynamic systems. Temperature scans can also be performed to track structural evolution as a function of thermal conditions.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Sample Environments:<\/h3>\n\n\n\n<p>Various sample environments are available to customize experiments, including temperature-controlled capillary holders, stopped-flow setups, and user-supplied sample environments. For detailed information on available sample environments, please visit the <a target=\"_blank\" href=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/home\/sample-environments\/\" rel=\"noreferrer noopener\">Sample Environments<\/a> page.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Remote Access and Mail-in Operations:<\/h3>\n\n\n\n<p>We offer remote access for SAXS data collection, enabling researchers to submit samples and collect data from anywhere. Mail-in options are also available, further facilitating access to the beamline.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Additional Capabilities:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Off-line Dynamic Light Scattering (DLS)<\/strong> and <strong>Refractive Index Measurements<\/strong>: These complementary techniques are available to enhance SAXS experiments. Reach out to the beamline staff in advance to schedule the measurements in the User lab during your visit. <\/li>\n\n\n\n<li><a href=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/home\/sample-environments\/time-resolved-saxs-tr-saxs\/\"><strong>Stopped-Flow SAXS<\/strong>:<\/a> Ideal for studying rapid biochemical reactions in solution with time-resolved SAXS.<\/li>\n<\/ul>\n\n\n\n<p>For a full list of parameters, including beamline specifications and detectors, please see our <a target=\"_blank\" href=\"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/parameters\/\" rel=\"noreferrer noopener\">Beamline Parameters<\/a> page.<\/p>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-65","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/pages\/65","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/comments?post=65"}],"version-history":[{"count":9,"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/pages\/65\/revisions"}],"predecessor-version":[{"id":11791,"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/pages\/65\/revisions\/11791"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/media?parent=65"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/small-angle-x-ray-scattering\/wp-json\/wp\/v2\/embl_taxonomy?post=65"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}