{"id":369,"date":"2021-04-12T11:00:23","date_gmt":"2021-04-12T11:00:23","guid":{"rendered":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/?page_id=369"},"modified":"2021-04-12T11:20:43","modified_gmt":"2021-04-12T11:20:43","slug":"sample-preparation","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/services-and-resources\/sample-preparation\/","title":{"rendered":"Sample preparation"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The SPC doesn&#8217;t provide protein purification service. However users can take advantage of the facility&#8217;s resources to purify their protein.<\/p>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"207\" height=\"300\" src=\"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-content\/uploads\/2021\/04\/PUC.png\" class=\"vf-summary__image\" alt=\"machine\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" \/>  <h3 class=\"vf-summary__title\">\n    \n    Preparative ultracentrifuges (Beckman, optima XPN-80)\n    <\/h3>\n\n  <p class=\"vf-summary__text\">Preparative ultracentrifugation is the method of choice for the isolation of membrane or macromolecular complexes.<\/p>\n<p class=\"vf-summary__text\"><em>Available in the wetlab at EMBL Hamburg under a previous request<\/em><\/p>\n<\/article>\n\n\n\n<h4 class=\"wp-block-heading\">Features:<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li>Maximum speed 80000 (rpm), 548300(RCF)<\/li><li>Temperature 5 to 40\u00b0C<\/li><li>Real-time run graphic<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Possible applications:<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li>Membrane harvesting and density gradient centrifugation<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Rotors:<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li>Type 45 Ti Rotor, fixe angle, titanium, 6 x 94ml, 45000 rpm, 235000 x g.<\/li><li>Type 42.2 Ti rotor, 42000 rpm<\/li><li>Rype 19 (fixed angle rotor), 19000 rpm,<\/li><li>SW 60 Ti, (Swinging bucket rotor), 60000 rpm,<\/li><\/ul>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"200\" height=\"300\" src=\"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-content\/uploads\/2021\/04\/AKTA.jpg\" class=\"vf-summary__image\" alt=\"machine\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" \/>  <h3 class=\"vf-summary__title\">\n    \n    AKTA Purifier (GE Healthcare)\n    <\/h3>\n\n  <p class=\"vf-summary__text\">Fast protein liquid chromatography is often used to analyze or purify mixtures of proteins. Our FPLC system is located in the cold room and is equiped with a deep well block fraction collector.<\/p>\n<\/article>\n\n\n\n<h4 class=\"wp-block-heading\">Possible applications<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li>Protein sample purification on site, SEC<\/li><li>Sample clean up before SAXS measurement<\/li><li>Equilibration of column prior to SAXS measurment using MALVERN-box<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Provided vs not provided:<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li>Deep well block are provided for fraction collection<\/li><li>Users should provide their own FPLC column and super loop if needed<\/li><\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":88,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-369","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/pages\/369","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/comments?post=369"}],"version-history":[{"count":7,"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/pages\/369\/revisions"}],"predecessor-version":[{"id":391,"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/pages\/369\/revisions\/391"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/pages\/88"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/media?parent=369"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/sample-preparation-characterisation\/wp-json\/wp\/v2\/embl_taxonomy?post=369"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}