{"id":14,"date":"2020-06-08T10:51:57","date_gmt":"2020-06-08T10:51:57","guid":{"rendered":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/home\/"},"modified":"2022-10-14T11:29:09","modified_gmt":"2022-10-14T11:29:09","slug":"home","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/","title":{"rendered":"Home"},"content":{"rendered":"<div class=\"vf-grid vf-grid__col-3 | vf-u-margin__bottom--800\">\n      <div class=\"vf-grid__col--span-2\">\n      <div class=\"vf-content-hub-html\">\n  <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=profiles&amp;filter-uuid=52a8aabf-02ba-4a86-893e-72a0a0b9fc4e&amp;pattern=node-teaser -->\n      <div data-embl-js-conditional-edit=\"28184\">\n              <h1 class=\"vf-lede\">The facility expresses and purifies proteins from\u00a0<em>E. coli<\/em>, insect cells and mammalian cells. For the recombinant protein purifications we implement a large array of chromatographic techniques. We also offer biophysical support for protein quality control, protein characterization and various types of interaction studies.<\/p>\r\n\n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/28184\" target=\"_blank\">Edit<\/a>\n    <\/div>\n  <\/div>\n    <\/div>\n      <div >\n\n<!-- <style>\n  .vf-content-hub-html {\n    --vf-stack-margin--custom: unset !important;\n  }\n<\/style> -->\n\n    <div class=\"vf-content-hub-html vf-stack vf-stack--600\" data-cache=\"5e1b1b36\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-value%5Bfield_person_positions.entity.field_position_membership%5D=leader&amp;filter-field-value%5Bfield_person_positions.entity.field_position_team.entity.field_foreignid%5D=143&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bteam%2Cmobile%2Cphones%5D=1&amp;limit=5&amp;pattern=vf-profile-inline&amp;sort-field-value%5Bchanged%5D=DESC -->\n                \n                            <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"77328\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60022227.jpg?itok=2w5hS27l\" alt=\"image of Kim Remans\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/kim-remans\" class=\"vf-profile__link\">Kim Remans<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-profile__job-title\">\n          Head of Protein Expression and Purification Core Facility\n        <\/p>\n      \n      \n      \n      \n      \n      \n              <p class=\"vf-profile__uuid\">\n          <span>ORCID:<\/span>\n          <a class=\"vf-profile__link vf-profile__link--secondary\" href=\"https:\/\/europepmc.org\/authors\/0000-0002-4394-7953\">\n            0000-0002-4394-7953\n          <\/a>\n        <\/p>\n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/77328\/77328\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n\n  <\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid embl-grid--has-sidebar\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>We strive to stay up-to-date with the latest scientific developments and trends in the field and test out new products and protocols for our users.&nbsp;<\/p>\n\n\n\n<p>We also maintain an extensive collection of expression vectors and bacterial strains and we have various frequently used proteins such as for example proteases and polymerases readily available.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid embl-grid--has-sidebar\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n  id=\"services\"  >\n        <a class=\"vf-section-header__heading vf-section-header__heading--is-link\"\n      id=\"services\"      href=\"\/groups\/protein-expression-purification\/services\">Services provided<svg aria-hidden=\"true\" class=\"vf-section-header__icon | vf-icon vf-icon-arrow--inline-end\" width=\"24\" height=\"24\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M0 12c0 6.627 5.373 12 12 12s12-5.373 12-12S18.627 0 12 0C5.376.008.008 5.376 0 12zm13.707-5.209l4.5 4.5a1 1 0 010 1.414l-4.5 4.5a1 1 0 01-1.414-1.414l2.366-2.367a.25.25 0 00-.177-.424H6a1 1 0 010-2h8.482a.25.25 0 00.177-.427l-2.366-2.368a1 1 0 011.414-1.414z\" fill=\"\" fill-rule=\"nonzero\"><\/path><\/svg><\/a>\n      <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<ul class=\"wp-block-list\"><li>Screening of expression constructs in different&nbsp;<em>E. coli<\/em>&nbsp;strains and culturing conditions using our automated liquid handling platform<\/li><li>Baculovirus-mediated protein expression in insect cells<\/li><li>Protein expression in mammalian suspension cultures<\/li><li>Purification of recombinant proteins using a variety of chromatographic techniques<\/li><li>Protein quality control<\/li><li>Characterization of proteins, protein complexes and the interactions of proteins with various types of molecules using biophysical techniques<\/li><li>Teaching and training of users and visitors in all aspects of protein expression, purification and biophysical characterization techniques<\/li><\/ul>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-box vf-box--is-link vf-box--normal vf-box-theme--primary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n              <a class=\"vf-box__link\" href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/user-guidelines\/\">\n                    User guidelines                <\/a>\n                  <\/h3> \n        <p class=\"vf-box__text\">For more detailed information about our services and how to access the facility, please have a look at our user guidelines.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-sidebar\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n  id=\"reagents\"  >\n  Reagent requests  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>We maintain a large database of <a href=\"https:\/\/grp-pepcore.embl-community.io\/vectors\/index.html\">expression vectors<\/a> at the EMBL Protein Expression and Purification Core Facility. Vectors that are commercially available can only be shared with internal EMBL users, but vectors generated at EMBL are freely available to the entire academic research community via a <a href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/05\/EMBL-MTA-02-2018-Vorlage-Remans-.doc\">Material Transfer Agreement<\/a>. Vectors that can be shared with external users are indicated in yellow in the <a href=\"https:\/\/grp-pepcore.embl-community.io\/vectors\/index.html\">vector tables<\/a>.<\/p>\n\n\n\n<p>If you\u2019re interested in obtaining reagents created at EMBL via the MTA, please complete the form and send a <strong>signed and dated pdf file<\/strong> to <a href=\"mailto:kim.remans@embl.de\">kim.remans@embl.de<\/a>. Since the contracting partners are the institutes (not the scientists) the contract has to be signed by yourself and by an official representative of your institute who is authorized to sign binding agreements. Please understand that we are not in a position to negotiate changes of the wording in the text of our <a href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/05\/EMBL-MTA-02-2018-Vorlage-Remans-.doc\">MTA<\/a>.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-sidebar\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n  id=\"technology\"  >\n  Technology  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>We are open to collaborations with academic and industrial partners to assess new products or technological developments. Furthermore, we are an active member of a network of protein facilities across Europe called Protein Production and Purification Partnership in Europe&nbsp;(<a rel=\"noreferrer noopener\" href=\"https:\/\/p4eu.org\/\" target=\"_blank\">P4EU<\/a>) to improve information exchange and the evaluation of novel technologies.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-box vf-box--is-link vf-box--normal vf-box-theme--primary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n              <a class=\"vf-box__link\" href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/protocols\/\">\n                    Protocols                <\/a>\n                  <\/h3> \n        <p class=\"vf-box__text\">In this section you can find some commonly used protocols.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-centered-content\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n    >\n        <a class=\"vf-section-header__heading vf-section-header__heading--is-link\"\n            href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/publications\/\">Publications<svg aria-hidden=\"true\" class=\"vf-section-header__icon | vf-icon vf-icon-arrow--inline-end\" width=\"24\" height=\"24\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M0 12c0 6.627 5.373 12 12 12s12-5.373 12-12S18.627 0 12 0C5.376.008.008 5.376 0 12zm13.707-5.209l4.5 4.5a1 1 0 010 1.414l-4.5 4.5a1 1 0 01-1.414-1.414l2.366-2.367a.25.25 0 00-.177-.424H6a1 1 0 010-2h8.482a.25.25 0 00.177-.427l-2.366-2.368a1 1 0 011.414-1.414z\" fill=\"\" fill-rule=\"nonzero\"><\/path><\/svg><\/a>\n      <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-summary vf-summary--publication\">\n    <h3 class=\"vf-summary__title\">\n        <a href=\"https:\/\/europepmc.org\/abstract\/MED\/33990604\" class=\"vf-summary__link\">\n        Quality control of protein reagents for the improvement of research data reproducibility.        <\/a>\n    <\/h3>\n    <p class=\"vf-summary__author\">\n      de Marco A, Berrow N, Lebendiker M, Garcia-Alai M, Knauer SH, Lopez-Mendez B, Matagne A, Parret A, Remans K, Uebel S, Raynal B    <\/p>\n    <p class=\"vf-summary__source\">\n    Nature communications        <span class=\"vf-summary__date\">2021<\/span>\n    <\/p>\n\n    <p class=\"vf-summary__text\">\n    doi:10.1038\/s41467-021-23167-z    <\/p>\n<\/article>\n\n\n\n<article class=\"vf-summary vf-summary--publication\">\n    <h3 class=\"vf-summary__title\">\n        <a href=\"https:\/\/europepmc.org\/abstract\/MED\/33781758\" class=\"vf-summary__link\">\n        Community-wide experimental evaluation of the PROSS stability-design method.        <\/a>\n    <\/h3>\n    <p class=\"vf-summary__author\">\n      Peleg Y, Vincentelli R, Collins BM, Chen KE, Livingstone EK, Weeratunga S, Leneva N, Guo Q, Remans K, Perez K, E K Bjerga G, Larsen \u00d8, Van\u011bk O, Sko\u0159epa O, Jacquemin S, Poterszman A, Kjaer S, Christodoulou E, Albeck S, Dym O, Ainbinder E, Unger T, Schuetz A, Matthes S, Bader M, de Marco A, Storici P, Semrau MS, Stolt-Bergner P, Aigner C, Suppmann S, Goldenzweig A, Fleishman SJ    <\/p>\n    <p class=\"vf-summary__source\">\n    Journal of molecular biology        <span class=\"vf-summary__date\">2021<\/span>\n    <\/p>\n\n    <p class=\"vf-summary__text\">\n    doi:10.1016\/j.jmb.2021.166964    <\/p>\n<\/article>\n\n\n\n<article class=\"vf-summary vf-summary--publication\">\n    <h3 class=\"vf-summary__title\">\n        <a href=\"https:\/\/europepmc.org\/abstract\/MED\/34140355\" class=\"vf-summary__link\">\n        Molecular basis of mRNA transport by a kinesin-1-atypical tropomyosin complex.        <\/a>\n    <\/h3>\n    <p class=\"vf-summary__author\">\n      Dimitrova-Paternoga L, Jagtap PKA, Cyrklaff A, Vaishali, Lapouge K, Sehr P, Perez K, Heber S, L\u00f6w C, Hennig J, Ephrussi A    <\/p>\n    <p class=\"vf-summary__source\">\n    Genes &#038; development        <span class=\"vf-summary__date\">2021<\/span>\n    <\/p>\n\n    <p class=\"vf-summary__text\">\n    doi:10.1101\/gad.348443.121    <\/p>\n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n  id=\"contact\"  >\n  Contact  <\/h2>\n      <p class=\"vf-section-header__subheading\">Protein Expression and Purification Core Facility<\/p>\n\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-grid | vf-grid__col-2\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-content-hub-html\" data-cache=\"44d86e56\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-contains%5Bfield_person_full_name%5D=Kim%20Remans&amp;filter-fields-empty=field_person_visible_internally&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bteam%2Corcid%5D=1&amp;limit=1&amp;pattern=vf-profile-inline -->\n                \n                            <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"77328\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60022227.jpg?itok=2w5hS27l\" alt=\"image of Kim Remans\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/kim-remans\" class=\"vf-profile__link\">Kim Remans<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-profile__job-title\">\n          Head of Protein Expression and Purification Core Facility\n        <\/p>\n      \n      \n      \n      \n      \n      \n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/77328\/77328\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<a href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/members\/\" target=\"_blank\">\n<button class=\"vf-button vf-button--primary vf-button--sm\">See all team members<\/button>\n<\/a>\n<!--\/vf-button-->\n\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-box vf-box--very-easy | vf-u-margin__bottom--400\">\n                            <p class=\"vf-box__text\">Phone: +49 6221 387- 8448<br \/>\nFax: +49 6221 387- 8519<br \/>\n<a href=\"mailto:pepcore@embl.de\" target=\"_blank\" rel=\"noopener\">pepcore@embl.de<\/a><\/p>\n<p class=\"vf-box__text\">EMBL Heidelberg<br \/>\nMeyerhofstr. 1<br \/>\n69117 Heidelberg<br \/>\nGermany<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-sidebar\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n  id=\"news\"  >\n        <a class=\"vf-section-header__heading vf-section-header__heading--is-link\"\n      id=\"news\"      href=\"\">Latest news<svg aria-hidden=\"true\" class=\"vf-section-header__icon | vf-icon vf-icon-arrow--inline-end\" width=\"24\" height=\"24\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M0 12c0 6.627 5.373 12 12 12s12-5.373 12-12S18.627 0 12 0C5.376.008.008 5.376 0 12zm13.707-5.209l4.5 4.5a1 1 0 010 1.414l-4.5 4.5a1 1 0 01-1.414-1.414l2.366-2.367a.25.25 0 00-.177-.424H6a1 1 0 010-2h8.482a.25.25 0 00.177-.427l-2.366-2.368a1 1 0 011.414-1.414z\" fill=\"\" fill-rule=\"nonzero\"><\/path><\/svg><\/a>\n      <\/h2>\n  <\/div>\n\n\n\n\n<p><\/p>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-content-hub-html\" data-cache=\"a4246e10\">\n  <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-all-fields=pepcore&amp;filter-content-type=article&amp;filter-field-value%5Bfield_target_display%5D=embl&amp;limit=2&amp;pattern=vf-news-item-default&amp;sort-field-value%5Bcreated%5D=DESC -->\n      <article class=\"vf-summary vf-summary--news\" data-embl-js-conditional-edit=\"154417\">\n\n              <span class=\"vf-summary__date\">08 Feb 2022<\/span>\n      \n                    <img decoding=\"async\" src=\"https:\/\/acxngcvroo.cloudimg.io\/v7\/https:\/\/www.embl.org\/news\/wp-content\/uploads\/2022\/02\/20210922_Heppenstall_1000x600px.jpg?w=600&amp;h=400&amp;force_format=jpeg&amp;q=30\" alt=\"News image\" class=\"vf-summary__image\" \/>\n      \n              <h3 class=\"vf-summary__title\">\n                      <a href=\"https:\/\/www.embl.org\/news\/lab-matters\/investing-in-italy-by-translating-embl-expertise-to-the-market\/\" class=\"vf-summary__link\">Investing in Italy by translating EMBL expertise to the market<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-summary__text\">\n                    Experiences at EMBL Rome led former group leader to establish his start-up in Italy, developing a new generation of gene therapies.\n        <\/p>\n      \n      <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/154417\" target=\"_blank\">Edit<\/a>\n\n    <\/article>\n      <article class=\"vf-summary vf-summary--news\" data-embl-js-conditional-edit=\"27253\">\n\n              <span class=\"vf-summary__date\">15 Apr 2020<\/span>\n      \n                    <img decoding=\"async\" src=\"https:\/\/acxngcvroo.cloudimg.io\/v7\/https:\/\/www.embl.org\/news\/wp-content\/uploads\/2020\/04\/Project01_Visual_WP.jpg?w=600&amp;h=400&amp;force_format=jpeg&amp;q=30\" alt=\"News image\" class=\"vf-summary__image\" \/>\n      \n              <h3 class=\"vf-summary__title\">\n                      <a href=\"https:\/\/www.embl.org\/news\/science\/producing-proteins-for-coronavirus-research\/\" class=\"vf-summary__link\">Producing proteins for coronavirus research<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-summary__text\">\n                    The Protein Expression and Purification Core Facility at EMBL Heidelberg will produce proteins for several coronavirus-related research projects, to assist the development of new strategies to fight t\u2026\n        <\/p>\n      \n      <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/27253\" target=\"_blank\">Edit<\/a>\n\n    <\/article>\n  <\/div>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"vf_template_front-page.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-14","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages\/14","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/comments?post=14"}],"version-history":[{"count":147,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages\/14\/revisions"}],"predecessor-version":[{"id":5908,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages\/14\/revisions\/5908"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/media?parent=14"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/embl_taxonomy?post=14"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}