{"id":1053,"date":"2020-08-26T10:15:14","date_gmt":"2020-08-26T10:15:14","guid":{"rendered":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/?page_id=1053"},"modified":"2021-08-02T12:41:45","modified_gmt":"2021-08-02T12:41:45","slug":"spectroscopy","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/services\/biophysical-support\/spectroscopy\/","title":{"rendered":"Spectroscopy"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Circular dichroism (CD) spectroscopy<\/h3>\n\n\n\n<p><strong>Used for:<\/strong> probing the secondary structure of chiral macromolecules and assessment of conformational stability, structural changes and complex formation by measuring the differential absorption of left and right-handed circularly polarized light. CD is also commonly used for protein quality control.<\/p>\n\n\n\n<p><strong>Wavelength range of our Jasco J-815 instrument:<\/strong> 190-900 nm.<\/p>\n\n\n\n<p><strong>Sample requirements:<\/strong> 80 \u00b5l of 0.5-2 mg\/ml of sample. Sample buffer should be compatible with CD measurements (buffer components absorbing in the region of interest should be avoided).<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>Jasco J-815 CD Spectrometer<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"vf-figure  | vf-figure--align vf-figure--align-centered  size-medium\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"200\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core35-300x200.jpeg\" alt=\"\" class=\"wp-image-1950\" srcset=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core35-300x200.jpeg 300w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core35-1024x683.jpeg 1024w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core35-768x512.jpeg 768w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core35-1536x1025.jpeg 1536w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core35-2048x1366.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/figure><\/div>\n\n\n<div class=\"vf-links vf-links--tight vf-links__list--s\">\n      <h3 class=\"vf-links__heading\">Useful links<\/h3>\n    <ul class=\"vf-links__list vf-list vf-links__list--secondary\">\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/www.jasco.de\/en\/content\/J-815-Outstanding\/~nm.13~nc.408\/Outstanding-Features-and-Benefits.html\">\n        Jasco J-815      <\/a>\n      <\/li>\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/jascoinc.com\/learning-center\/theory\/spectroscopy\/circular-dichroism-spectroscopy\/\">\n        Jasco \u2013 circular dichroism theory      <\/a>\n      <\/li>\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/jascoinc.com\/learning-center\/best-practices\/circular-dichroism-tips-tricks\/\">\n        Jasco \u2013 circular dichroism tips and tricks      <\/a>\n      <\/li>\n  <\/ul>\n\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Fluorescence spectroscopy<\/h3>\n\n\n\n<p><strong>Used for:<\/strong> probing structural changes (folding, assembly, stability) in macromolecules and assessment of macromolecular interactions by measuring the intensity of emitted photons from a sample after it has absorbed photons. It is an in-solution method that makes use of intrinsic fluorophores (Trp, Tyr, Phe) or fluorescent dyes.<\/p>\n\n\n\n<p>Fluorescence polarization is the phenomenon were the light emitted by a fluorophore has unequal intensities along different axes of polarization. This can be used to measure the binding of relatively small fluorescent molecules to their larger target molecule. It is an in-solution method that makes use of fluorescent dyes.<\/p>\n\n\n\n<p><strong>Wavelength range of our Jasco FP-8500 instrument:<\/strong> 200-750 nm.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>Jasco FP-8500 Fluorescence Spectrometer<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"vf-figure  | vf-figure--align vf-figure--align-centered  size-medium\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"200\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core3-300x200.jpeg\" alt=\"\" class=\"wp-image-1952\" srcset=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core3-300x200.jpeg 300w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core3-1024x683.jpeg 1024w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core3-768x512.jpeg 768w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core3-1536x1025.jpeg 1536w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core3-2048x1366.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/figure><\/div>\n\n\n<div class=\"vf-links vf-links--tight vf-links__list--s\">\n      <h3 class=\"vf-links__heading\">Useful links<\/h3>\n    <ul class=\"vf-links__list vf-list vf-links__list--secondary\">\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/www.jasco.de\/Spectroscopy\/Fluoreszenz\/FP-8000Serie\/FP-8500\">\n        Jasco FP-8500      <\/a>\n      <\/li>\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/jascoinc.com\/learning-center\/theory\/spectroscopy\/fluorescence-spectroscopy\/\">\n        Jasco \u2013 fluorescence spectroscopy theory      <\/a>\n      <\/li>\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/jascoinc.com\/learning-center\/best-practices\/fluorescence-tips-tricks\/\">\n        Jasco \u2013 fluorescence tips and tricks      <\/a>\n      <\/li>\n  <\/ul>\n\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">UV-Vis spectroscopy<\/h3>\n\n\n\n<p><strong>Used for:<\/strong> measuring the absorbance spectrum of molecules, determining the concentration of molecules and monitoring enzymatic reactions. It measures a molecule\u2019s light absorption at different wavelengths.<\/p>\n\n\n\n<p><strong>Wavelength range of our Agilent Cary60 instrument:<\/strong> 190-1100 nm.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>Agilent Cary60 UV-Vis Spectrometer<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"vf-figure  | vf-figure--align vf-figure--align-centered  size-medium\"><a href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core5-scaled.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"200\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core5-300x200.jpeg\" alt=\"\" class=\"wp-image-1954\" srcset=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core5-300x200.jpeg 300w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core5-1024x683.jpeg 1024w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core5-768x512.jpeg 768w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core5-1536x1025.jpeg 1536w, https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-content\/uploads\/2021\/06\/PEP_Core5-2048x1366.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure><\/div>\n\n\n<div class=\"vf-links vf-links--tight vf-links__list--s\">\n      <h3 class=\"vf-links__heading\">Useful links<\/h3>\n    <ul class=\"vf-links__list vf-list vf-links__list--secondary\">\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/www.agilent.com\/en\/product\/molecular-spectroscopy\/uv-vis-uv-vis-nir-spectroscopy\/uv-vis-uv-vis-nir-systems\/cary-60-uv-vis-spectrophotometer?gclid=EAIaIQobChMI6YnfkK3Q8AIVheJ3Ch0OkgXfEAAYASAAEgIiIfD_BwE&#038;gclsrc=aw.ds#zoomELIBRARY_1030015\">\n        Agilent Cary60 UV-Vis      <\/a>\n      <\/li>\n  <\/ul>\n\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":1051,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-1053","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages\/1053","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/comments?post=1053"}],"version-history":[{"count":20,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages\/1053\/revisions"}],"predecessor-version":[{"id":2168,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages\/1053\/revisions\/2168"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/pages\/1051"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/media?parent=1053"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/protein-expression-purification\/wp-json\/wp\/v2\/embl_taxonomy?post=1053"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}