{"id":219,"date":"2025-02-13T22:49:27","date_gmt":"2025-02-13T22:49:27","guid":{"rendered":"https:\/\/www.embl.org\/groups\/mobile-labs\/?page_id=219"},"modified":"2025-03-04T11:34:08","modified_gmt":"2025-03-04T11:34:08","slug":"confocal-laser-scanning-microscope-lsm900-zeiss","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/mobile-labs\/equipment\/confocal-laser-scanning-microscope-lsm900-zeiss\/","title":{"rendered":"Confocal laser scanning microscope – LSM900, Zeiss"},"content":{"rendered":"\n
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Living as well as chemically fixed specimens can be imaged. Specific structures such as membranes or cytoskeleton can be stained with different fluorescent dyes and observed simultaneously. <\/p>\n\n\n\n

Scanning through a stack of optical sections allows 3D reconstruction of the specimen.<\/p>\n\n\n\n

AI-guided acquisition allows for overview imaging with automated zoom-in or photomanipulation jobs on regions of interest.<\/p>\n\n\n\n

Objectives <\/strong><\/h3>\n\n\n\n
    \n
  • Objective EC Plan-Neofluar 2.5x\/0.085 M27 air (FWD=8.8 mm)<\/li>\n\n\n\n
  • Objective Plan-Apochromat 10x\/0.45 M27 air (FWD=2.1 mm) <\/li>\n\n\n\n
  • Objective Plan-Apochromat 20x\/0.8 M27 air (FWD=0.55 mm)<\/li>\n\n\n\n
  • Objective Plan-Apochromat 40x\/1.3 Oil DIC M27 (FWD=0.13 mm)<\/li>\n\n\n\n
  • Objective Plan-APOCHROMAT 63x\/1,4 Oil DIC (FWD=0.19 mm)<\/li>\n<\/ul>\n\n\n\n

    Laser:<\/strong><\/h3>\n\n\n\n
      \n
    • 405 nm \u2013 5 mW<\/li>\n\n\n\n
    • 488 nm \u2013 10 mW<\/li>\n\n\n\n
    • 561 nm \u2013 10 mW<\/li>\n\n\n\n
    • 640 nm \u2013 5 mW<\/li>\n<\/ul>\n\n\n\n

      Confocal detectors:<\/strong><\/h3>\n\n\n\n
        \n
      • 3 Gallium Arsenide Phosphid-PMT (GaAsP-PMT) for fluorescence<\/li>\n\n\n\n
      • standard PMT as transmission detector<\/li>\n<\/ul>\n\n\n\n

        WF-Filters:<\/strong><\/h3>\n\n\n\n
          \n
        • EX BP 385\/30 for DAPI <\/li>\n\n\n\n
        • EX BP 469\/38 for FITC <\/li>\n\n\n\n
        • EX BP 555\/30 for TRITC <\/li>\n\n\n\n
        • EX BP 631\/33 for Cy5 <\/li>\n\n\n\n
        • QBS 405+493+575+653 <\/li>\n\n\n\n
        • EM QBP 425\/30+514\/30+592\/25+709\/100<\/li>\n<\/ul>\n\n\n\n

          Light Source HXP Lamp 120C (widefield)<\/strong><\/h3>\n\n\n\n

          Mercury short-arc reflector lamp<\/strong> 120 W. Ideal light<\/strong> source for fluorescence<\/strong> applications in microscopy. Integrated electronic shutter.<\/p>\n\n\n\n

          Camera: (widefield)<\/strong><\/h3>\n\n\n\n

          Camera Axiocam 503 mono<\/p>\n\n\n\n

          Number of Pixels: 1936 (H) x 1460 (V) = 2.8 Megapixels Pixel size: 4.54 \u03bcm x 4.54 \u03bcm Chip size: 8.8 mm x 6.6 mm, equivalent to 2\/3″ (11 mm diagonal) Spectral range: With protection glass app. 400 nm to 1000 nm Max. Full Well Capacity: Approx. 15.000 e<\/p>\n\n\n\n

          Stage STEP Set for LSM:<\/strong><\/h3>\n\n\n\n
            \n
          • Scanning Stage 130×100 STEP with stepper motor (2 mm spindle pitch) incl. Stepper controller SMC 2009, Joystick XY, fixed motor cable with exit on stage left back side and universal mounting frame K (160 mm x 110 mm), also suitable for Z-Piezo <\/li>\n\n\n\n
          • travel range: 130 mm x 100 mm (adjustable) <\/li>\n\n\n\n
          • max. speed: 50 mm\/s – resolution: 0.1 \u03bcm <\/li>\n\n\n\n
          • reproducibility: +\/- 1 \u03bcm <\/li>\n\n\n\n
          • absolute accuracy: +\/- 5 \u03bcm<\/li>\n<\/ul>\n\n\n\n

             Stage attachment Z PIEZO WSB 500 (D)<\/strong><\/h3>\n\n\n\n
              \n
            • travel range in Z: 500 \u03bcm <\/li>\n\n\n\n
            • max speed: 5 mm\/sec <\/li>\n\n\n\n
            • resolution: 1 nm <\/li>\n\n\n\n
            • reproducibility: +\/- 5 nm<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n
              \n
              \n\n
              \"\"
              Confocal Microscope, LSM900, Zeiss. Credit: Massimo Del Prete\/EMBL<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
              <\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":159,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-219","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/pages\/219","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/comments?post=219"}],"version-history":[{"count":5,"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/pages\/219\/revisions"}],"predecessor-version":[{"id":321,"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/pages\/219\/revisions\/321"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/pages\/159"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/media?parent=219"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mobile-labs\/wp-json\/wp\/v2\/embl_taxonomy?post=219"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}