Microscopy Facility

Advanced light microscopy support and technology development

Widefield Leica DMI6000 B

Location: Room 42


Automated inverted microscope for biomedical research, ideal for fluorescence



Leica DMI 6000 CS AFC Bino inverted microscope with motorized XY-stage


MagnificationNATypeImmersionCoverslipWD (mm)
5x0.15HCX PL FluotarDry– / 0.17 
10x0.30HC PL FluotarDry– / 0.1711
20x0.50HC PL FluotarDry– / 0.171.15
40x0.75HCX PL FluotarDry0.400.75
63x1.4-0.6HC PL ApoOil0.170.60

Fluorescent source

Leica EL6000External light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours)

Fluorescence filter sets

NameExcitation filterEmission filterDichroic
ABP 340 – 380 nmLP 430 nm400 nm
CFPBP 436/20 nmLP 515 nm455 nm
L5BP 480 – 656 nmLP 590 nm580 nm
YFPBP 500/20 nmBP 535/30  nm515 nm
N2.1BP 515 – 560 nmLP 590 nm580 nm
Y5BP 620/60 nmBP 700/75  nmY5


Leica AF6000 64bit Z control and SW Autofocus, multichannel acquisition, stitching


  • Leica DFC345 FX C mount 0.55 x HC
  • Leica DFC 450 C mount 0.55 x HC. CCD 2/3″ da 2560×1920 px

Power up and down procedure

  • Please contact Alvaro Crevenna to schedule a session
  • Report problems on the Log file

Book this resource online (intranet)