Location: Room 42

Description

Automated inverted microscope for biomedical research, ideal for fluorescence

Specifications

Microscope

Leica DMI 6000 CS AFC Bino inverted microscope with motorized XY-stage

Objectives

Magnification	NA	Type	Immersion	Coverslip	WD (mm)
5x	0.15	HCX PL Fluotar	Dry	– / 0.17
10x	0.30	HC PL Fluotar	Dry	– / 0.17	11
20x	0.50	HC PL Fluotar	Dry	– / 0.17	1.15
40x	0.75	HCX PL Fluotar	Dry	0.40	0.75
63x	1.4-0.6	HC PL Apo	Oil	0.17	0.60

Fluorescent source

Leica EL6000External light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours)

Fluorescence filter sets

Name	Excitation filter	Emission filter	Dichroic
A	BP 340 – 380 nm	LP 430 nm	400 nm
CFP	BP 436/20 nm	LP 515 nm	455 nm
L5	BP 480 – 656 nm	LP 590 nm	580 nm
YFP	BP 500/20 nm	BP 535/30  nm	515 nm
N2.1	BP 515 – 560 nm	LP 590 nm	580 nm
Y5	BP 620/60 nm	BP 700/75  nm	Y5

Software

Leica AF6000 64bit Z control and SW Autofocus, multichannel acquisition, stitching

Cameras

• Leica DFC345 FX C mount 0.55 x HC
• Leica DFC 450 C mount 0.55 x HC. CCD 2/3″ da 2560×1920 px

Power up and down procedure

• Please contact Alvaro Crevenna to schedule a session
• Report problems on the Log file

Book this resource online (intranet)